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Mohammad Majdi, Ghasem Karimzadeh, Mohammad A. Malboobi, Reza Omidbaigi, and Ghader Mirzaghaderi

the identification of ploidy level by flow cytometry (FCM) (PAI; Partec, Münster, Germany); Rosa victoriana (2 n = 2 x = 14) was used as a standard plant. Nuclei were extracted from leaves according to Yokoya et al. (2000) . Leaf material from the

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Sandra B. Wilson, Gary W. Knox, Keona L. Muller, Rosanna Freyre, and Zhanao Deng

analysis and hybridization potential. Ploidy level of the porterweed cultivars was analyzed by flow cytometry ( Viloria and Grosser, 2005 ). Several young, recently matured leaves were collected from containerized stock plants and a small piece of leaf

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Hirotoshi Tsuda, Hisato Kunitake, Mai Yamasaki, Haruki Komatsu, and Katsunori Yoshioka

crosses between colchicine-induced tetraploid shashanbo and highbush blueberry ‘Spartan’; 2) to confirm the hybridity and ploidy level of putative hybrids using morphological observation, molecular markers, flow cytometry, and chromosome counts; and 3) to

Open access

Kenneth W. Leonhardt

. Today, more researchers rely on flow cytometry (FCM) for the screening of treated plant material and ploidy-level confirmation. FCM allows for the quantification of plant nuclear DNA, subsequently providing the user with the ploidy level of the samples

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Richard J. Henny, James R. Holm, Jianjun Chen, and Michelle Scheiber

aforementioned morphological evaluation, the 73 plants were evaluated by measuring stomata length and then analyzed by flow cytometry. To measure stomata length, stomata of plants were “fixed” onto a glass slide using an epidermal peel. One drop of Super Glue

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Davut Keleş, Hasan Pınar, Atilla Ata, Hatıra Taşkın, Serhat Yıldız, and Saadet Büyükalaca

nutrient medium ( Fig. 2 ). Fig. 2. Development of haploid plants via anther culture. ( A ) Plant formation in petri dishes, ( B, C ) rooting of plants obtained via anther culture in culture tubes, ( D ) acclimation of plants. Flow cytometry studies. The

Open access

Hamidou F. Sakhanokho, Nurul Islam-Faridi, and Barbara J. Smith

soil-containing pots in the greenhouse for flow cytometry analysis and cytology investigation. Fully developed seedlings ( Fig. 1 ) were used to harvest leaves and root tips for flow cytometry and cytology studies, respectively. Fig. 1. Ziziphus

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Sumin Kim, Mengqiao Han, and A. Lane Rayburn

agreement with lower genome sizes of C. arietinum . In addition, the chromosome size data of Ohri and Pal (1991) is more consistent with the genome sizes obtained in this study. Whether this is due to differences in technologies between flow cytometry and

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Hamidou F. Sakhanokho and M. Nurul Islam-Faridi

tetraploid (2 n = 4 x = 48) nature of the suspected polyploid plant by comparing control diploid (2 n = 2 x = 24) plants with the suspected tetraploid (2 n = 4 x = 48) cytotype using flow cytometry analysis, stomatal dimensions, chloroplast numbers, and

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Mark K. Ehlenfeldt and James J. Polashock

cultivars (e.g., ‘Duke’, ‘Hannah’s Choice’, etc.). Table 1. Plant materials used in Vaccinium section Hemimyrtillus investigations. Flow cytometry determination of DNA content of plant nuclei (C-value). Leaf material (≈1 cm 2 /20 to 50 mg) together with