A 100% pine bark medium was amended with dolomitic lime at 0, 3, or 6 kg·m−3 and periodically fertilized with 210 ml of a nutrient solution containing 100 ppm N as (NH4)2S04. At the 3- and 6-kg lime treatments, medium solution NH4-N concentrations decreased rapidly while N03-N concentrations increased. At 0 kg lime, the NH4-N decrease was slower than at the 3- and 6-kg lime treatments and N03-N was not found. Similarly treated bark without plants was used to determine a N03-N accumulation rate (NAR). NAR was greatest at 6 kg of lime, except at the last 2 sampling dates, when NAR did not differ between 3 and 6 kg of lime. This lack of difference was attributed to a limiting NH4-N supply at 6 kg of lime. In a 2nd experiment, NAR of bark treated with 6 kg of lime per m3 and fertilized with 300 ppm NH4-N was 3 times greater than with bark treated with 100 ppm NH4-N, thus supporting the contention that, over time, the NH4-N supply of the 100-ppm treatment limited nitrification. These results indicate that the stimulative influence of lime on nitrification is subject to medium pH and NH4-N status that changes over time.