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Allison D. Oakes, Tyler Desmarais, William A. Powell, and Charles A. Maynard

, requiring many steps and different growing media ( Gonçalves et al., 1998 ; Gonzalez Padilla et al., 2003 ; Pijut et al., 2010 ). The main production bottleneck for micropropagated american chestnuts are the rooting and acclimatization stages, where shoots

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Carlos Alberto Lecona-Guzmán, Sheila Reyes-Zambrano, Felipe Alonso Barredo-Pool, Miguel Abud-Archila, Joaquín Adolfo Montes-Molina, Reiner Rincón-Rosales, and Federico Antonio Gutierrez-Miceli

or the outgrowth of embryos into plants, and the acclimatization of those plants to ex vitro conditions ( Pospíšilová et al., 1999 ). Callus culture might be a useful technique to cultivate A. americana , offering the potential to produce plants of

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Huan Xiong, He Sun, Feng Zou, Xiaoming Fan, Genhua Niu, and Deyi Yuan

) Acclimatized plants under greenhouse conditions for 2 months. Adventitious bud induction. The cotyledonary node explants were excised from 3-week-old seedlings cultured in the tube and placed on MS medium containing 0.65% agar and 3% sucrose, supplemented with

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Ze Li, Xiaofeng Tan, Zhiming Liu, Qing Lin, Lin Zhang, Jun Yuan, Yanling Zeng, and Lingli Wu

different hormonal combinations on Camellia oleifera shoot elongation and strengthening in WPM. Rooting and acclimatization. When elongated, adventitious shoots reached 3–4 cm in length; they were cut off at the base and transferred to a half-strength MS

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Samir C. Debnath and Danny L. Barney

experiment was conducted three times. Rooting and acclimatization. Elongated shoots (4- to 5-cm long) from BM with zeatin (2.3 or 4.6 μM) were transferred to BM void of PGR for rooting in vitro. Rooted shoots were planted in 45-cell plug trays (5.9 cm cell

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Zhenghui Tang, Honghui Lin, Lei Shi, and Weilun Chen

sand (bar = 1.5 cm). ( G ) Plants acclimatized in greenhouse (bar = 4 cm). For basal leaf segment induction, results were similar to those for medium leaf explants ( Fig. 1B ). Basal leaf segment showed direct somatic embryogenesis (DSE) without

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Wagner A. Vendrame, Ian Maguire, and Virginia S. Carvalho

-vitro shoot induction and multiplication in cultures of Doritaenopsis . Plant regeneration, acclimatization, survival, and establishment were also evaluated. Materials And Methods Plant material and explant sterilization. Plants of

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Yeh-Jin Ahn and Grace Qianhong Chen

(day/night) photoperiod with light supplemented by metal halide lighting at a photon flux density of 1000 to 1250 μmol·m −2 ·s −1 . Transparent plastic covers were placed over the plantlets for the first 2 weeks for acclimatization. Histological

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Rashid Sultan Al-Obeed, Salah El-Hendawy, Mayada Kadri Seliem, and Khadija Rabeh Al-Harbi

recorded from 10 shoots after 8 weeks of culturing. In vitro rooting and acclimatization. Shoots (2.5–4 cm) were used for rooting in full or half strength MS basal medium supplemented with 0, 0.5, or 1.0 mg·L −1 IBA or NAA. Rooting parameters including

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Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut

elongation of the eighth replicate. (c 1 ) Elongated shoot excision. (c 2 ) Internode segments. (c 3 ) Callus induction. (d) PLB induction. (e) Shoot formation. (f) Plant regeneration. (g) Acclimatization in the greenhouse. Callogenesis. After