Technologies, Santa Clara, CA). All measurements were determined three times, and all parameters were analyzed in triplicate. HPLC analysis of monomeric anthocyanins in grape skins. Only monomeric anthocyanins of fully mature grapes (harvested 24 Aug. 2018
Ying Wang, Tingting Xue, Xing Han, Lingxiao Guan, Liang Zhang, Hua Wang, and Hua Li
Tuan Anh Le, Zoltán Pék, Sándor Takács, András Neményi, Hussein G. Daood, and Lajos Helyes
-homogenized tomato by crushing in a crucible mortar and shaking for 15 min with 3% metaphosphoric acid solution. The mixture was filtered through a filter paper and purified by a 45-μm nylon syringe filter before injection on to the HPLC column. A Chromaster liquid
Dean A. Kopsell, Kimberly J. Whitlock, Carl E. Sams, and David E. Kopsell
Technologies, Santa Clara, CA) before HPLC analysis. Purslane shoot tissue pigment HPLC analysis. An HPLC unit with a photodiode array detector (1200 series; Agilent Technologies) was used for purslane pigment separation. Chromatographic separations were
william M. Walter Jr.
The sugar content of the sweetpotato cultivars Centennial, Coroner, Georgia Red, Jewel, and Sweet Red was measured by nign performance liquid chromatography (HPLC) and compared to the sugar content found by measuring the refractive index of cellular sap and converting the refractive index value to sugar concentration. The sugar content and refractive index values were measured for just-harvested, cured and stored roots. Changes in the sugar content as determined by refractive index were found to be linearly related to changes 1n sugar content measured by HPLC, indicating that this method can be used to monitor changes in postharvest sugar content.
Alley E. Watada and Tony T. Tran
A high performance liquid chromatography (HPLC) method was developed for analyzing vitamin B1, and the method was used with other HPLC methods to monitor vitamins C, B1, and B2 of fresh fruits and vegetables stored at different temperatures. Ascorbic acid content of most commodities either dropped after being placed in storage or decreased during storage. Thiamine increased during storage of ‘Tendergreen’ beans and decreased in ‘BelRus’ potatoes; whereas riboflavin decreased in ‘Tendergreen’ beans and increased in ‘Superior’ potatoes. The changes in other cultivars or commodities were not significant.
W.Y.L. Poon and I.L. Goldman
1 Former graduate assistant. We thank Peter Vanderveer for assisrtance with HPLC analyses, Marilyn H.Y. Hovius for assistance with figures, and W.F. Tracy and T. Sharkey for helpful comments.
John A. McCallum, Meeghan Pither-Joyce, and Martin Shaw
This research was funded by the New Zealand Foundation for Research, Science and Technology. We thank Ruth Butler for statistical assistance, Lyall Simmons for HPLC analysis, and Jane Lancaster (AgriFood Solutions) for critical reading of this
V.I. Shattuck, Y. Kakuda, B.J. Shelp, and N. Kakuda
Abbreviations: GS, glucosinolate; HPLC, high-performance liquid chromatography. 1 Dept. of Horticultural Science. 2 Dept. of Food Science. We gratefully acknowledge E.W. Underhill for performing the LC plasma spray mass spectrometry analysis and
Joshua K. Craver, Joshua R. Gerovac, Roberto G. Lopez, and Dean A. Kopsell
Econofilter PTFE 25/20; Agilent Technologies, Wilmington, DE) using a 5-mL syringe (Becton, Dickinson and Co., Franklin Lakes, NJ) before HPLC analysis. Carotenoid and chlorophyll HPLC analysis. An HPLC unit with a photodiode array detector (1200 series
Yuko Yoshizawa, Kenji Sakurai, Satoru Kawaii, Masayoshi Asari, Junichi Soejima, and Noboru Murofushi
Aqueous ethanol extracts prepared from 19 apple (Malus ×domestica Borkh.) cultivars were studied to explore their antiproliferative activity. Half of them showed strong inhibition on proliferation of human leukemic HL-60 cells, while the others were weak. Total polyphenols, 1, 1-diphenyl-2-picrylhydrazyl (DPPH) radical scavenging activity, and total anthocyanins were measured and the results indicated that the antiproliferative activity was more strongly correlated to the polyphenols and radical scavenging activity than to the anthocyanin content. Several polyphenols in `Jonathan' were identified and quantified by high-performance liquid chromatography (HPLC) analysis. Among those compounds found during HPLC, catechin and epicatechin seemed partially responsible for HL-60 antiproliferation. A careful examination on parentage of the apple cultivars tested revealed that `Jonathan' and its progeny showed high antiproliferation toward HL-60. This is the first observation about the relationship between antiproliferative activity and parentage of apples, and the information would be useful to create new apple cultivars that posses more anticancer potential.