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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe, and Sheri B. Crabtree

. Microsatellite-enriched libraries and primer design. Genetic Identification Services (GIS, Chatsworth, CA) constructed pawpaw genomic libraries from DNA extracted from the cultivar PA-Golden (#1); libraries were enriched for di-nucleotide repeat GA (Library B

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Gehendra Bhattarai and Shawn A. Mehlenbacher

from conserved sequences that flank the repeat region. At polymorphic loci, PCR amplification results in different product sizes. SSR markers are preferred over several other marker types because of their high level of polymorphism, multiple alleles

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Zahra Noormohammadi, Mehdi Hosseini-Mazinani, Isabel Trujillo, Luis Rallo, Angjelina Belaj, and Majid Sadeghizadeh

alleles is a consequence of sequence polymorphisms in the flanking regions of the locus resulting from point mutations or insertion/deletions ( Jones and Ardren, 2003 ). Discrimination and identification of Iranian olive cultivars. The study of

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David Jesús Gil-Ariza, Iraida Amaya, José Manuel López-Aranda, José Federico Sánchez-Sevilla, Miguel Ángel Botella, and Victoriano Valpuesta

. Results Simple sequence repeat marker diversity. A panel of 92 F . × ananassa accessions was genotyped with 10 polymorphic EST-SSRs allowing the identification of several alleles per locus ( Table 2 ). Four EST-SSRs (ChFaM002, ChFaM005, ChFaM

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Samuel F. Hutton, Jay W. Scott, and David J. Schuster

relied on the transfer of resistant alleles from related wild species into the cultivated tomato, because no resistance has been found in the S. lycopersicum germplasm. Resistance to TYLCV has been discovered in numerous wild tomato species, including

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Deborah Dean, Phillip A. Wadl, Xinwang Wang, William E. Klingeman, Bonnie H. Ownley, Timothy A. Rinehart, Brian E. Scheffler, and Robert N. Trigiano

escaping cultivation and hybridizing with the natively occurring V. dentatum (J. Feely, personal communication). This caveat necessitates the availability of a reliable method for identification of this popular ornamental shrub and its hybrids. A group

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Melinda A. Miller-Butler, Barbara J. Smith, Brian R. Kreiser, and Eugene K. Blythe

; Lerceteau-Köhler et al., 2003 ), which allows breeders to identify dominant and recessive alleles for these loci. The advent of molecular genetics provided plant breeders with molecular markers to use for identification of alleles linked to disease

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Barbara Gilmore, Nahla Bassil, April Nyberg, Brian Knaus, Don Smith, Danny L. Barney, and Kim Hummer

using SSRs as molecular markers for identity verification. SSRs are easy to use, codominant, multiple allelic, abundant, and highly reproducible across laboratories for genotype identification ( Powell et al., 1996 ). Application of this technique to

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Carlos Miranda, Jorge Urrestarazu, Luis G. Santesteban, José B. Royo, and Valero Urbina

significant, differentiation. The identification of private alleles, the clustering of all accessions with unique alleles in only one group, and remarkable differences in allelic richness among groups constitute further evidence of the uniqueness of this

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Jung Hyun Kwon, Ji Hae Jun, Eun Young Nam, Kyeong Ho Chung, Ik Koo Yoon, Seok Kyu Yun, and Sung Jong Kim

flowers is also used to determine cross-compatibility ( Beppu et al., 2003 ). The PCR-based S-RNase allele typing has been used widely to determine self-incompatibility among groups. This method allows identification of different S -alleles based on