Search Results

You are looking at 71 - 80 of 152 items for :

  • Refine by Access: All x
Clear All
Free access

Rhiana F. Jones, Paul W. Bosland, Robert L. Steiner, Richard W. Jones, and Mary A. O’Connell

as a disease responsive gene in a screen for PR genes expressed during the HR of C. annuum leaves to Tobacco mosaic virus (TMV) ( Shin et al., 2001 ); the Universal Stress Protein, is similar to a bacterial stress responsive gene found throughout

Open access

Trent J. Davis, Miguel I. Gómez, Scott J. Harper, and Megan Twomey

hop, as well as cachexia in citrus, and fruit marking symptoms on some stonefruit (Hayata et al., 2017; Marquez-Molins et al., 2021). HSVd is one of the most significant virus-like pathogens of hop production, first reported in Japan, and more

Full access

Keun H. Cho, Joo Young Kim, Maria I. Alvarez, Veronica Y. Laux, Lauren K. Valad, Joshua M. Tester, Thomas A. Colquhoun, and David G. Clark

at the University of Florida, which has a constitutive figwort mosaic virus (FMV) 35S promoter and NOS-terminator. After the nucleotide sequence of each plasmid construct was confirmed with Sanger sequencing service (Genewiz, South Plainfield, NJ

Free access

Xiaojuan Zong, Jiawei Wang, Li Xu, Hairong Wei, Xin Chen, Dongzi Zhu, Yue Tan, and Qingzhong Liu

, 48, and 72 h, respectively, after treatment, immediately frozen in liquid nitrogen, and stored at −80 °C for RNA extraction. Plantlets cultured in the normal MS medium at 25 °C with a 16/8-h photoperiod served as the control for the qRT-PCR analysis

Free access

Tingting Zhao, Jingkang Hu, Yingmei Gao, Ziyu Wang, Yufang Bao, Xiaochun Zhang, Huanhuan Yang, Dongye Zhang, Jingbin Jiang, He Zhang, Jingfu Li, Qingshan Chen, and Xiangyang Xu

, re-amplifications were applied using the primers with added restriction site sequences ( Eco RI and Bam HI). Target products were excised from the gel, purified and cloned into tobacco rattle virus RNA2 (TRV2), which was digested with restriction

Free access

Gordon J. Lightbourn, John R. Stommel, and Robert J. Griesbach

. RNA analysis. Flavonoid gene expression [MYC, MYB, and WD transcription factors, anthocyanin synthase ( Ans ), dihydroflavonol reductase ( Dfr ), and chalcone synthase ( Chs )] was compared under inductive and noninductive environments for

Free access

Wanmei Jin, Jing Dong, Yuanlei Hu, Zhongping Lin, Xuefeng Xu, and Zhenhai Han

-strength vitamins, 30 g·L −1 sucrose, 6 g·L −1 agar, and 0.2 mg·L −1 IBA. Transformation and regeneration of grape explants. The DREB1b gene was isolated from Arabidopsis and placed under the control of the cauliflower mosaic virus (CaMV) 35S promoter in the

Free access

Wenjing Guan, Xin Zhao, Richard Hassell, and Judy Thies

melon necrotic spot virus in cucurbits was a significant advantage over soil fumigation with methyl bromide, which does not control this viral disease ( Cohen et al., 2007 ). Meanwhile, tomato yellow leaf curl virus , tomato spotted wilt virus , and

Free access

John R. Stommel, Gordon J. Lightbourn, Brenda S. Winkel, and Robert J. Griesbach

area of absorption per gram of fresh weight of three replicates. Gene expression. Flowers, immature fruit, and leaves were harvested from actively growing plants, frozen in liquid nitrogen, and stored at −80 °C. Total RNA was isolated from ≈100

Free access

Jollanda Effendy, Don R. La Bonte, and Niranjan Baisakh

thickness (1.2 mm) at 0 (control), 2, 4, 8, and 12 h after skinning and the peels were immediately frozen in liquid nitrogen and stored at –80 °C for RNA extraction. Three independent roots were used for each time point as replicates. RNA Isolation, cDNA