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Juan M. Quintana, Helen C. Harrison, James Nienhuis, and Jiwan P. Palta

Flow rate, Ca content, and Ca concentration of sieve sap were measured at four developmental stages (flowering and 1, 2, and 3 weeks after flowering) in six commercial snap bean cultivars to better understand physiological factors associated with genetic differences for pod Ca concentration. Sampling began 5 weeks after greenhouse planting and consisted of 1) decapitation of the plant at the first node; 2) covering the stem with preweighed dry cotton; and 3) removing the cotton, reweighing it, and saving it for Ca determination. Flow rate was defined as the difference in cotton weight (expressed as milliliter) per 12 hours. Ca determinations were made using an atomic absorption spectrophotometer. Calcium content was defined as milligram of Ca per total volume of sieve sap after 12 hours. Concentration of Ca was the quotient of Ca content by flow rate (expressed as milligrams Ca per milliliter sap). A positive correlation between flow rate and total Ca content of sieve sap (R 2 = 0.83), flow rate and Ca concentration of sieve sap (R 2 = 0.36), and Ca content and Ca concentration (R 2 = 0.80) were found. Maturity appeared to be an important factor affecting flow rate and Ca influx in snap bean plants. Significant differences between genotypes for Ca content and flow rate were observed. High Ca genotypes reflected a high flow rate regardless developmental stage.

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Juan M. Quintana, Helen C. Harrison, James Nienhuis, and Jiwan P. Palta

We have previously observed significant variation for pod Ca concentration among snap bean genotypes. In the present experiment, we compare pod Ca concentration between snap bean and dry bean genotypes. Eight snap bean cultivars and eight dry bean cultivars were chosen to be evaluated for pod Ca concentration in summers of 1995 and 1996 at Hancock, Wis. The experimental design consisted in randomized complete blocks with three replications in 1995 and six in 1996. Snap and dry beans were planted in June and hand-harvested in August for both experiments. Soil analysis showed 430 ppm of Ca in soil at time of planting. No additional Ca was applied. Plots consisted of 10 plants each. Harvesting was made by collecting a pooled sample of medium size pods from the 10 plants. Ca determinations were made using an atomic absorption spectrophotometer. Data was presented as mg of Ca per gram of dry weight, pooled from both years, and analyzed using SAS. Results reflected significant differences between genotypes. Checkmate (5.5) showed the highest pod calcium concentrations and Labrador (3.9) the lowest among snap beans. G0122 (5.1) resulted in the highest and Porrillo (3.6) the lowest within dry beans Results were consistent across years. Snap beans (4.6) presented significantly higher pod calcium concentration than dry beans (4.2). Apparently, snap bean genotypes have the ability to absorb calcium from the soil more efficiently than dry bean genotypes, and this phenomenon is not significantly influenced by environmental factors.

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Juan M. Quintana, Helen C. Harrison, Jiwan P. Palta, and James Nienhuis

To understand physiological factors associated with genetic differences for pod Ca concentration between snap bean genotypes, flow rate and Ca uptake of sieve sap were measured, as well as pod Ca concentration. Measurements for flow rate and Ca uptake were done at three developmental stages (fl owering and 1 and 3 weeks after) in two commercial snap bean cultivars (Hystyle and Labrador) grown aeroponically. Pods were collected 2 weeks after flowering only. Flow rate and Ca uptake sampling began 4 weeks after transplanting and consisted of: 1) decapitation of the plant at the first node; 2) covering the stem with pre-weighed dry cotton; and 3) removing the cotton, reweighing it, and saving it for Ca determination. Flow rate was defined as the difference in cotton weight (expressed as ml) per 17 hr divided by foliage mass. Ca uptake was defined as mg of Ca per total volume of sieve sap after 17 hr divided by foliage mass. Ca determinations were made using an atomic absorption spectrophotometer. A positive correlation between flow rate and total Ca uptake of sieve sap (R 2 = 0.90), flow rate and pod Ca concentration (R 2 = 0.47), and Ca uptake and pod Ca concentration (R 2 = 0.42) were found. Hystyle reflected 1.5 times more flow rate and pod Ca concentration than Labrador. Significant differences between genotypes for pod Ca concentration, Ca uptake, and flow rate were observed. Results were consistent across developmental stages.

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E. Arnaud-Santana, D.P. Coyne, J.R. Steadman, A.K. Vidaver, K.M. Eskridge, and J.S. Beaver

Heritabilities (H) of seed transmission and leaf and pod reactions to common bacterial blight (CBB) Xanthomonas campestris pv. phaseoli (Xcp) and to web blight (WB) Thanatephorus cucumeris (Tc) were studied. The reaction to CBB was quantitatively inherited. H values of .36, .46, and .34 for leaf reaction, .14, .12, and .27 for pod reaction, .53, .26, and .36 for seed transmission were estimated based on variation of F6 lines derived from bean crosses 'PC-50' × XAN-159, 'PC-50'× BAC-6, and 'Venezuela 44' × BAC-6 (greenhouse, NE). No significant correlations were detected between leaf and pod reactions or between pod reaction and seed transmission. Quantitative inheritance patterns were observed for leaf reactions to Xcp, Tc, and architecture (AR) in F6lines from the cross BAC-6 × HT 7719 (field, Dominican Republic). H values of .23 (CBB), .14 (WB), and .30 (AR) were obtained. No significant correlations were detected between CBB with WB or AR. A low correlation (+.22) was found between WB and AR.

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Armando Campos Cruz, Douglas C. Scheuring, and J. Creighton Miller Jr.

The effect of Biozyme™, a commercial germination stimulant, on emergence of bean and sweet corn seeds, treated with four levels of Carbofuran and Chlorothalonil, and grown under suboptimal field temperatures, was evaluated. Half the seeds from each treatment were treated with Biozyme™ Two planting dates were selected to provide suboptimal temperatures during emergence. Pesticide overdoses caused significant detrimental effects to all emerging seedlings. These effects were magnified under the low temperatures of the first planting. Biozyme™ treatment significantly improved emergence rate, percent emergence, final stand and number of ears of sweet corn in the first planting, and the percent emergence final stand, plant dry weight, and number of ears in the second planting. In beans, however, Biozyme™ treatment significantly reduced emergence rate, percent emergence. and final stand in the first planting, while significantly increasing percent emergence, plant dry weight, and seed dry weight in the second planting. The beneficial effects of Biozyme™ appeared to be independent of the negative effects of pesticide overdoses.

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Juan M. Quintana, Helen C. Harrison, James Nienhuis, and Jiwan Palta

Pod stomatal density and Ca concentration levels were analyzed for six commercial snap bean cultivars harvested at four planting dates in an attempt to find morphological traits that are related to cultivar differences in pod Ca concentration. The experimental layout was a randomized complete-block design with two replications per planting date, all grown in one location. Snap beans were planted at 1 week intervals beginning 9 June 9 1995 and were harvested in August. Sampling consisted of five pod sizes (1, 2, 3, 4, and 5 according to commercial standards) from each genotype. Stomatal countings were performed using a microscope linked to a television camera. Determinations for pod Ca concentration were made using an atomic absorption spectrophotometer. No differences were detected for pod Ca concentration among planting dates, although there were differences for pod Ca concentration and stomata density among cultivars. Pod stomatal density was positively correlated to pod Ca concentration (R 2 = 0.60), while pod maturity appeared to be negatively correlated to pod Ca concentration (R 2 = 0.37) and pod stomatal density (R 2 = 0.49).

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H.M. Ariyarathne, Dermot P. Coyne, and Geunhwa Jung

Halo blight (HB), brown spot (BS), and rust incited by the bacterial pathogens Pseudomonas syringae pv. phaseolicola (Psp), Pseudomonas syringae pv. syringae (Pss) and the fungal pathogen Uromyces appendiculatus, respectively, are important diseases of common beans. The objectives were to construct a RAPD linkage map, and to locate HB and BS resistance genes and genes for some other traits. One-hundred-seventy RAPD markers were mapped in 78 RI lines of the cross BelNeb 1 and A 55. Eleven main and nine minor linkage groups were identified. MAPMAKER/QTL, interval mapping, was used to identify genomic regions involved in the genetic control of the traits. One region was found to control HB leaf reactions to strain HB16 while three regions controlled reactions to strain HB 83. These regions accounted for 22% and 18%, 17%, and 17% of phenotypic variation of resistance, respectively. Four putative QTLs were identified for resistance to BS, and accounted for 37%, 26%, 23%, and 19% of the phenotypic variation. Rust resistance was determined by a single major gene to both rust strains US85NP 5-1 and D82vc74fh. However, linked markers were not identified. The V gene controlling flower and stem color was tightly linked with the Operon marker O10.620.

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Juan M. Osorno, Carlos G. Muñoz, James S. Beaver, Feiko H. Ferwerda, Mark J. Bassett, Phil N. Miklas, Teresa Olczyk, and Bill Bussey

resources. Plant breeders have developed and released common bean ( Phaseolus vulgaris ) cultivars with resistance to BGYMV ( Beaver and Miklas, 1999 ; Beebe, 1994 ; Rosas et al., 1997 ) and researchers have identified specific resistance genes such as

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John Rojas, Julian Quintero, Yhors Ciro, and Javier Silva

calories ( Valdez-Perez et al., 2011 ). These legumes are so vital for human nutrition that ≈12 million metric tons of Phaseolus vulgaris are consumed every year worldwide. Moreover, in 2014, the United States produced more than 86,700 t of kidney beans

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Marko Maras, Barbara Pipan, Jelka Šuštar-Vozlič, Vida Todorović, Gordana Đurić, Mirjana Vasić, Suzana Kratovalieva, Afrodita Ibusoska, Rukie Agić, Zdravko Matotan, Tihomir Čupić, and Vladimir Meglič

. 2010 Beans in Europe: Origin and structure of the European landraces of Phaseolus vulgaris L Theor. Appl. Genet. 121 829 843 Angioi, S.A. Rau, D. Nanni, L. Bellucci, E. Papa, R. Attene, G. 2011 The genetic make-up of the European landraces of the