without ion-exchange purification) were analyzed using HPLC (Agilent Technologies, Santa Clara, CA) equipped with a 1100 series binary pump, fitted with a 2 × 150-mm column (Synergi 4μ Hydro-RP80A, serial no. 106273-5; Phenomenex, Torrance, CA). The mobile
Muhammad Mansoor Javaid, Manish Bhan, Jodie V. Johnson, Bala Rathinasabapathi, and Carlene A. Chase
Valentina Schmitzer, Maja Mikulic-Petkovsek, Franci Stampar, and Vlasta Cunja
(Chromafil AO-45/25; Macherey-Nagel, Düren, Germany), and analyzed on an HPLC system (Thermo Finnigan Surveyor; ThermoFisher Scientific, San Jose, CA) with a diode array detector at 350 nm (flavonols) and 530 nm (anthocyanins). The column was a HPLC column
William M. Walter Jr.
The sugar content of five sweetpotato [Ipomoea batatas (L.) Lam.] cultivars (`Centennial', `Cordner', `Georgia Red', `Jewel', and `Sweet Red') was measured by high-performance liquid chromatography (HPLC) and compared to the sugar content of the cellular sap measured by refractive index (RI). The HPLC and RI sugar contents were measured at harvest, after curing, and during storage. Changes in the sugar content, as determined by the RI, were found to be linearly related to changes in the sugar content of cell sap and tissue, as measured by HPLC, indicating that this method can be used to monitor changes in postharvest total sugar content.
Philipp von Bieberstein, Ya-ming Xu, A.A. Leslie Gunatilaka, and Raphael Gruener
:10 with MeOH and subjected to HPLC on a Kromasil C 18 RP column (250 × 4.6 mm, 5 μm, 0.7 mL·min −1 flow rate) with gradient elution using 40% to 100% aqueous MeOH and ultraviolet-Vis detection at 229 nm. An external standard curve method was used to
Samson Zeray Tesfay, Sakhile Mathe, Albert T. Modi, and Tafadzwanashe Mabhaudhi
radical scavenging ability of the extract was expressed as μmol Trolox equivalents per gram of plant material on dry basis. Nonstructural soluble CHOs. Sugars were determined using HPLC-refractive-index detector (RID) according to Liu et al. (1999) , with
Ying Wang, Tingting Xue, Xing Han, Lingxiao Guan, Liang Zhang, Hua Wang, and Hua Li
Technologies, Santa Clara, CA). All measurements were determined three times, and all parameters were analyzed in triplicate. HPLC analysis of monomeric anthocyanins in grape skins. Only monomeric anthocyanins of fully mature grapes (harvested 24 Aug. 2018
Tuan Anh Le, Zoltán Pék, Sándor Takács, András Neményi, Hussein G. Daood, and Lajos Helyes
-homogenized tomato by crushing in a crucible mortar and shaking for 15 min with 3% metaphosphoric acid solution. The mixture was filtered through a filter paper and purified by a 45-μm nylon syringe filter before injection on to the HPLC column. A Chromaster liquid
Dean A. Kopsell, Kimberly J. Whitlock, Carl E. Sams, and David E. Kopsell
Technologies, Santa Clara, CA) before HPLC analysis. Purslane shoot tissue pigment HPLC analysis. An HPLC unit with a photodiode array detector (1200 series; Agilent Technologies) was used for purslane pigment separation. Chromatographic separations were
william M. Walter Jr.
The sugar content of the sweetpotato cultivars Centennial, Coroner, Georgia Red, Jewel, and Sweet Red was measured by nign performance liquid chromatography (HPLC) and compared to the sugar content found by measuring the refractive index of cellular sap and converting the refractive index value to sugar concentration. The sugar content and refractive index values were measured for just-harvested, cured and stored roots. Changes in the sugar content as determined by refractive index were found to be linearly related to changes 1n sugar content measured by HPLC, indicating that this method can be used to monitor changes in postharvest sugar content.
W.Y.L. Poon and I.L. Goldman
1 Former graduate assistant. We thank Peter Vanderveer for assisrtance with HPLC analyses, Marilyn H.Y. Hovius for assistance with figures, and W.F. Tracy and T. Sharkey for helpful comments.