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Abdulrhman I. Al-hemaid and David S. Koranski

Petunia `Red Flash', Vinca `Little Blanch', Pansy `Magestic Giant Purple', and Impatien `Super Elfin Red' plugs were held in the greenhouse after they reached the saleable size in 200, 406, 512, and 800 for 1 to 3 weeks Pansy plugs were held in coolers at 40, 50, or 60F under fluorescent light for 16 hrs photoperiod for 1 to 3 weeks in 200, 406, 512, or 800 plug trays. All plants ware transplanted weekly and were grown in the greenhouse until flowering and data were collected. For plants bald in the greenhouse, plants were affected by transplanting time. As the holding time increased the final height, diameter, flower number, and fresh and dry weight of plants decreased. The flowering time was delayed by increase the holding time, regardless of plant variety, As cell size decreased, plant height, diameter, flower number, and fresh and dry weight decreased. For plants held in the coolers, the flowering time was delayed by the transplant time, regardless of cooler temperatures Plant quality was not affected by the treatment. The height, diameter, flowers number, and fresh and dry weight of plants showed a little effects by temperatures, cell size, and transplanting time.

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Sandra Menasha, Milton Tignor, and David Heleba

Transplanting sweet corn is commonly practiced in the northeast U.S. to improve stand establishment and promote early harvest. However, early spring storms and labor constraints can delay transplanting when establishment is most desirable. `Temptation' sugary enhanced (se) sweet corn transplants 0-, 2-, 4-, 6-, and 8-days-old beyond the “grower” 2-week growth period were field planted to explore the effects delayed planting combined with plug cell volume differences would have on transplant ear quality and early yields. The transplant treatments were evaluated in a two-way factorial (five delayed planting dates × three plug volumes) arranged in a split-plot design with five replications. Field sites were the whole plot treatment and the factorial treatments were the split-plots. All transplants were planted on 24 May 2004 at the two field sites. The final density was ≈22,000 plants/acre. Transplant cell volume (15, 19, and 29 mL) had no significant effect on ear quality and total marketable yield. Ear length was significantly affected by field site (P≤ 0.0001) and ear diameter was significantly affected by planting delay (P= 0.0145). Field site (P≤ 0.0001) and planting delay (P= 0.0090) both significantly affected the number of early marketable ears/acre. The results indicate that transplants can remain in the plug cells up to 20 days (2 weeks + 6 day delay) before the delay negatively impacts ear diameter, tip fill, and early marketable yield.

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K. Francis Salifu, Douglass F. Jacobs, Guillermo Pardillo, and Mary Schott

We examined growth and nitrogen (N), phosphorus (P), potassium (K), and microelement nutrition of grafted black walnut (Juglans nigra L.) seedlings exposed to increasing nutrient supply and grown in the greenhouse for 18 week. Plants were potted and grafted within the first 4 week, then fertigated once each week for a 7-week period with a varying nutrient solution of 20N–4.4P–16.6K that delivered 0, 1160, 2320, and 4620 mg N/plant. Plants were harvested at week 18. There was a positive mean growth response to increased fertilization, although trends were statistically similar across treatments. Leaf nutrient concentration ranged from 22 to 31 g · kg–1 N, 5 to 14 g · kg–1 P, and 19 to 25 g · kg–1 K. The 2320 mg N/plant treatment increased leaf nutrient content 18% to 86% for N, 33% to 303% for P, and 23% to 58% for K compared with the control. Nitrogen efficiency decreased with increased N supply. Increased nutrient retention in the growing medium at higher fertility suggests root plugs could serve as immediate critical nutrient sources for grafted black walnut seedlings after outplanting. Study results suggests nursery fertilization can be used to improve the nutritional quality of grafted black walnut as well as store nutrients in root plugs for later utilization to benefit early establishment success.

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W. Tietjen, P.J. Nitzsche, W.P. Cowgill Jr., M.H. Maletta, and S.A. Johnston

`Market Prize' and `Bravo' cabbage (Brassica oleracea Var. capitata L.), transplanted as peat plug and bareroot plants into a field naturally infested with Plasmodiophora brassicae, Woronin, were treated immediately after planting with a liquid or a granular surfactant. APSA 80™, applied in transplant water, significantly reduced percent clubbing and disease severity index (DSI) compared to control treatments. Miller Soil Surfactant Granular™ did not significantly reduce percent clubbing or DSI. There was a significant effect of cultivar on percent clubbing and DSI. There was no significant effect of transplant type on percent clubbing or DSI. This year's study culminates five years of investigation of surfactants for clubroot control. Specific surfactants have proven to be an effective control of clubroot in cabbage. Chemical names used: nonylphenoxypolyethoxyethanol (APSA 80™); alpha-alkanoic-hydro omega-hydroxy poly (oxyethylene) (Miller Soil Surfactant Granular™).

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Jack W. Buxton, Wenwei Jia, and Guoqiang Hou

The automatic subirrigation system consists of a capillary mat placed above a constant water level in a reservoir. The optimum mat height above the water level was established by slanting a flat surface so the difference in vertical height from one end of the surface to the other was 25 cm. A ground cover providing water movement but not root penetration was placed over the mat. The capillary mat extended beyond the lowest end of the slopped surface and into the reservoir, the mat at the lowest end of the slopped surface was at the same vertical height as the water in the reservoir and remained constantly saturated. Plug trays were placed at intervals of 2.5 cm in vertical height above the water level. An average of 96-100% germination was obtained with marigold, tomato, impatiens and pepper seed in trays placed 5-7.5 cm in vertical height above the water level. These seedlings continued to develop and reached transplanting stage quicker than other trays. The rate and % germination was less in trays placed on the surface nearer to the height of the water in the reservoir. Germination in trays above 12.5 cm was greatly reduced and seed that did germinate did not develop and eventually died.

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Yoshiaki Kitaya, Genhua Niu, Maki Ohashi, and Toyoki Kozai

Artificial lighting is widely used in controlled environment plant production to enhance plant growth and quality. However, high light intensity with artificial lighting is costly, and often causes increase of leaf temperature and, thus, leaf burn. We investigated the effects of photosynthetic photon flux (PPF) and photoperiod on the growth and morphogenesis of lettuce plug transplants under ambient and enriched CO2 levels. Three days after seeding, the plants were cultured under four PPF levels (100, 150, 200, and 300 μmol·m–2·s–1), two photoperiods (16 and 24 hr), and two CO2 levels (400 and 800 μmol·mol–1) for 18 days in growth chambers. Light source was fluorescent lamps. The air temperature around the plants was kept at 20°C. The results showed that dry weight of the plants increased linearly as PPF and daily integrated PPF (product of PPF and photoperiod) increased under both CO2 levels. At the same daily integrated PPF, higher CO2 level and longer photoperiod led to higher dry weight of the plants. CO2 enrichment increased significantly dry weight of the plants. The ratio of T/R and specific leaf area of the plants decreased quadratically as daily integrated PPF increased under both CO2 levels. The ratio of leaf length to leaf width of the plants decreased quadratically as PPF increased under the two photoperiods and CO2 levels.

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Allen M. Armitage

Plugs of Arabis `Spring Cham', Cerastium `Columnae', Salvia `Blue Queen', and Saxifraga `Purple Robe' (128 plugs/tray) were cooled at 2 to 3C for 0, 4, or 8 weeks. Plugs were potted in 10-cm containers and placed in warm (20C day, 17C night) or cool (10C day, 8C night) and provided with various long- and short-day combinations. In general, all taxa flowered more rapidly in the warm house, but were not significantly taller. Arabis showed no response to cooling or photoperiodic treatments, but Cerastium and Saxifraga had significantly longer internodes when subjected to increasing number of LD cycles. Plants of Salvia and Cerastium also flowered more rapidly when subjected to increasing cycles of LD, but height of Salvia and Arabis was reduced when LD were followed by SD. Other species of perennials will also be discussed.

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Holly L. Scoggins, Paul V. Nelson, and Douglas A. Bailey

Substrate solution testing is an essential management tool for greenhouse plug production. Current methods of plug solution extraction and testing can be confounded by subjective aspects of their techniques. The press extraction method (PEM) developed at North Carolina State University offers a convenient and timely method of solution extraction. The rooting substrate is brought to container capacity and after a period of one hour, pressing the plug surface with a finger or thumb is sufficient to expel the solution. This series of experiments serves to quantify possible variation that may occur in pH, Electrical conductivity (EC), and nutrient analysis from differing manual extraction forces. A modified press was designed to apply a range of force [53, 71, 89, 106, and 124 N (5.0, 6.7, 8.3, 10.0, and 11.6 lb/inch2)], and sampling protocol consistency was verified. For all three experiments, the range of extraction forces within a single fertilizer rate did not significantly affect solution pH or EC. When testing included a range of fertilizer rates, results were significantly different among the fertilizer rates, demonstrating the method's ability to detect changes in pH and EC resulting from increases in fertility levels. Nutrient analysis (NO3 -, NH4 +, P, K, Ca, Mg, Na, B, Cu, Fe, Mn, and Zn) of solution extracted from two different rooting substrates (peat-based and coir-based) showed no differences within substrates for the range of force treatments.

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Stephanie E. Burnett, Svoboda V. Pennisi, Paul A. Thomas, and Marc W. van Iersel

Polyethylene glycol 8000 (PEG-8000) was applied to a soilless growing medium at the concentrations of 0, 15, 20, 30, 42, or 50 g·L-1 to impose controlled drought. Salvia (Salvia splendens F. Sellow. ex Roem & Shult.) seeds were planted in the growing medium to determine if controlled drought affects morphology and anatomy of salvia. Polyethylene glycol decreased emergence percentage and delayed emergence up to 5 days. Stem elongation of salvia treated with the five lowest concentrations was reduced up to 35% (21 days after seeding), and salvia were a maximum of 53% shorter and the canopy was 20% more narrow compared to nontreated seedlings 70 days after seeding. These morphological changes were attributed to PEG-8000 mediated reduction in leaf water potential (Ψw). The growing medium Ψw ranged from -0.29 to -0.85 MPa in PEG-8000 treated plants, and plant height was positively correlated with Ψw 21 days after seeding. Stem diameter of PEG-treated seedlings was reduced up to 0.4 mm mainly due to reductions in vascular cross-sectional area. Xylem cross-sectional area decreased more than stem and phloem cross-sectional area. Polyethylene glycol 8000 reduced vessel element number, but not diameter.