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-cultivar quality parental line that is homozygous for the southern root-knot nematode resistance gene. Origin In 1995, recurrent backcross breeding procedures were initiated to transfer the dominant southern root-knot nematode resistance gene from a Scotch Bonnet

incorporate the dominant root-knot nematode resistance gene ( N ) into the classical pimento-type “Perfection” genetic background ( Fig. 1 ) ( Fery and Dukes, 1996 ; Hare, 1957 ). The donor parent of the N resistance gene was ‘Mississippi Nemaheart’ and the

al., 1994 ; Lawrence et al., 1986 ), there is a lack of information on how infection affects RSA in either susceptible or resistant genotypes. Thus, it is important to understand the relationship between RSA and nematode resistance in sweetpotato

commercial hybrid, ‘Tygress’. ‘Tygress’ contains a very large wild-species introgression (≈30 Mb) on chromosome 6—which couples the S. peruvianum nematode-resistance introgression ( Mi gene) together with the S. chilense begomovirus

3 recombinant inbred line (RIL) developed from the commercial hybrid, Tygress. This hybrid is heterozygous for a very large wild-species introgression (≈30 Mb) on chromosome 6, which resulted from recombining nematode resistance ( Mi ) with

for root knot nematode resistance and the I-3 gene for fusarium wilt race 3 resistance. Replicated plots of the F 3 generation were grown in two growers’ fields in coastal South Carolina in 2001 under conditions of heavy natural infection by TSWV to

against the Pp whole genome sequence (Version 1.0; Washington State University, 2010a ) was used to infer their location on specific chromosomes. The RGAs with same alignment results were thought as a “false” RGA. Results Root-knot nematode resistance

severe damage on root-knot nematode-resistant soybean ‘Forrest’ and sweetpotato ‘CDH’ cultivars ( Fargette, 1987 ; Fargette et al., 1996 ) and on tomato ‘Rossol’, which contains the Mi-1 nematode resistance gene ( Prot, 1984 ). Brito et al. (2004

Growers Company (Rocky Ford, CO) ( Fig. 1 ). PA-593 was derived from a single BC 3 F 4 plant grown in 2014, and is homozygous for the dominant N nematode resistance gene. The N gene conditions a high level of resistance against several RKNs, including

were unsuccessful ( Deakin et al., 1971 ; Fassuliotis, 1977 , 1979 ; Walters and Wehner, 2002 ). An alternative strategy may be through comparative genetic mapping of target genes such as nematode resistance in C. metuliferus using cross