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R.N. Trigiano and R.A. May

A tissue culture laboratory exercise illustrating regeneration of whole plants from leaf segments of chrysanthemum by organogenesis is described. Using simple, common media, shoots can be generated in 5 weeks and rooted after an additional 3 weeks. Acclimatization of plants can be accomplished in a simple mistbed in the greenhouse. The exercise is adaptable to depict genotype differences among cultivars, optimization of shoot induction, effects of growth regulators, and experimental design. Callus is typically not formed during shoot formation; however, co-cultivation of leaf segments with a virulent strain of Agrobacterium tumefaciens produces callus with a strain of disarmed A. tumefaciens harboring NPTII construct affects regeneration of plants resistant to kanamycin.

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Virginia M. Keith and Mark H. Brand

Significant occurrences of phenotypic variation have been noted in micropropagated Rhododendron. Studies were undertaken to determine what aspects of micropropagation lead to variation. Rhododendron `Molly Fordham' was used to evaluate growth parameters and the incidence of variation in plants that originated from 3 month and 54 month old cultures. Plants from 3-month-old cultures were significantly wider than plants from 54-month-old cultures. Rhododendron `Aglo', `Molly Fordham', and `Scintillation' were used to evaluate growth and the incidence of variation in plants grown from microcutting bases and rerooted microcutting tips (retips). Three-month-old retips were significantly taller and wider than bases of the same age, but possessed fewer branches. The influence of in vitro N6-[2-isopentenyl]adenine (2-iP) concentration on the growth and phenotype of regenerated plants of `Aglo', `Molly Fordham', and `Scintillation' was examined. Data taken 3 months post-acclimation indicate that growth and the incidence of variation in response to 2-iP concentration is cultivar dependent.

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T.J. Smalley, M.A. Dirr, A.M. Armitage, B.W. Wood, R.O. Teskey, and R.F. Severson

Abbreviations: CK, cytokinins; LWP, leaf water potential. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate

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Yan Xu and Bingru Huang

both genetically and environmentally regulated senescence are associated with hormonal changes with either upregulating or downregulating effects ( Nooden and Leopoid, 1988 ). Ethylene, abscisic acid (ABA), and cytokinins are three major phytohormones

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Guochen K. Png, Katherine S. Downes, and Beng H. Tan

feasibility of shoot micropropagation of L. macrantha has yet to be investigated. The provision of exogenous cytokinins to the explant through the culture medium at the shoot multiplication stage stimulates the formation of microshoots ( Hartmann et al

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Fumiomi Takeda, Thomas Tworkoski, Chad E. Finn, and Charles C. Boyd

the distal half to two-third of the axillary bud should remove the differentiated meristem. Cytokinin application to buds favors shoot formation and inhibits root initiation in stem cuttings, but in decapitated pea ( Pisum sativum ) cuttings

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Asma Alhussein Alawaadh, Yaser Hassan Dewir, Mona S. Alwihibi, Abdulhakim A. Aldubai, Salah El-Hendawy, and Yougasphree Naidoo

regenerated shoots were kept routinely by subculturing once every 4 weeks on MS medium without PGR and used as plant materials to conduct the experiments. Culture conditions. The cytokinins and auxins were added to MS basal medium, thereafter, the pH of all

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Ying-Chun Chen, Chen Chang, and Huey-Ling Lin

inherent tissue culture-induced problems, such as leaf chlorosis and growth retardation, and to increase production ( Monteiro et al., 2000 ). Cytokinins are a class of plant growth regulators widely used in Passiflora tissue culture. Benzyladenine (BA

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Bruce W. Wood

squares ANOVA at P ≤ 0.05. Mean separation for main effects and interaction using Student's t test. Study 2: Influence of cytokinin and auxin on flowering Expt. 1: Influence of benylamino purine, triiodobenzoic acid, and naphthaleneacetic acid on

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Fatemeh Haddadi, Maheran Abd Aziz, Hossein Kamaladini, and Seyed Ali Ravanfar

. Thidiazuron, a substituted phenylurea with its cytokinin- and auxin-like effects, is the most recently studied of the cytokinins ( Debnath and Teixeira da Silva, 2007 ). There are many reports of improved regeneration capacity with TDZ compared with other