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Xiaoling Jin, Xijun Hu, Youping Sun, Donglin Zhang, and Ping He

number of shoots per explant were recorded after 6 weeks. Rooting and acclimatization. Regenerated shoots (≈2 cm) were transferred onto a rooting medium: WPM supplemented with 0.27, 0.54, 2.7, or 5.4 μM NAA or 0.25, 0.5, 2.5, or 5.0 μM IBA.WPM without any

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Juan Bernardo Pérez-Hernández and María José Grajal-Martín

and vitamins, 2.74 m m glutamine, and 30 g·L −1 sucrose ( Ara et al., 1999 ). Plantlet development was carried out during two cycles of 14 d under these conditions. The pH of all media was adjusted to 5.8 before autoclaving. For acclimatization, in

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Salah El-Hendawy, Abdullah A. Alsadon, Mayada Kadry Seliem, and Yougasphree Naidoo

terms of number of axillary shoots per explant, shoot length, and fresh weight of whole explants were recorded after 6 weeks of culture. All measurements were obtained from 10 randomly chosen shoots. In vitro rooting and acclimatization. Shoots (2–3 cm

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Rochelle R. Beasley and Paula M. Pijut

. Acclimatization of rooted plants. After 6 weeks on root induction medium, rooted plantlets were transplanted into 10 cm × 9-cm plastic pots containing a moist, autoclaved, soilless medium with high porosity (Premier ProMix HP; Premier Horticulture Inc., Quakertown

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Margarita Pérez-Jiménez, Alfonso Guevara-Gázquez, Antonio Carrillo-Navarro, and José Cos-Terrer

light (45 μmol·m −2 ·s −1 ; GRO-LUX, Sylvania, Surrey, UK) photoperiod, and after 10 d, data for the roots and stems were recorded before acclimatization. The number of germinated seeds was also recorded. Statistical analysis. Data were first tested for

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Samir C. Debnath

containing 35 mL gelled BM with 1 μM zeatin. There were four jars per treatment for each clone and each jar contained five explants. The experiment was conducted three times. Rooting and acclimatization. Elongated shoots (3 to 4 cm long) of all

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Jalil Dejampour, Islam Majidi, Solmaz Khosravi, Sevil Farhadi, and Atena Shadmehr

rootstock explants. z Rooting and acclimatization. For rooting, 2 to 3 cm long microshoots were cultured in DKW medium containing half-strength of macroelements plus IBA ( Table 2 ) or NAA (similar concentrations to IBA) for 1 week in the dark. Shoots were

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Flávia D. Pereira, José Eduardo B.P. Pinto, Luciana D.S. Rosado, Helen C.A. Rodrigues, Suzan K.V. Bertolucci, and Osmar A. Lameira

26 ± 1 °C. After 30 d incubation, the effects of residual PGRs were assessed by determining the numbers and lengths of the plantlets and etiolated shoots regenerated from the different types of explants. Rooting and acclimatization (hardening

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Kaitlin J. Palla, Rochelle R. Beasley, and Paula M. Pijut

with 5 μM zeatin and routinely micropropagated through nodal sections to increase the number of clonal shoots available for rooting trials. Rooting of microshoots and acclimatization of plants to the greenhouse. Microshoots (2 to 3 cm in length) were

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Beatrice Nesi, Debora Trinchello, Sara Lazzereschi, Antonio Grassotti, and Barbara Ruffoni

formation. When roots had developed, bulblets (plants) were planted in pots filled with peat:perlite substrate (1:1 by volume) and acclimatized in a greenhouse maintained at 80% relative humidity controlled by an electronic leaf and 50% shading. After 15 d