sd s were obtained from three biological replications. Petal vacuolar pH measurement. To measure the vacuolar pH, 2 g of fresh petals of the Pl and Pd flowers at the fully opened stage was ground in liquid nitrogen and centrifuged at 18,407 g n for
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Qianqian Shi, Long Li, Lin Zhou, and Yan Wang
Tongyin Li, Guihong Bi, Judson LeCompte, T. Casey Barickman, and Bill B. Evans
maintained at 30 °C using a thermostat column compartment. All separations were achieved using gradient mobile phase of 1) reverse osmosis water adjusted to pH 2.5 with trifluoroacetic acid and 2) acetonitrile. The gradient held the following percentages: 15
C. Siobhan Dunets and Youbin Zheng
) and concentration of other nutrients, samples were acidified to pH ≈2 using H 2 SO 4 and filtered through a 0.45-µm syringe filter (09-719 F; Thermo Fisher Scientific Inc., Waltham, MA). Samples for determination of soluble PO 4 -P only were not
William R. Okie and Bryan Blackburn
rack of 12 twigs per cultivar. Vials were filled with Floralife Crystal Clear (Floralife Inc., Walterboro, SC) at double strength (2× = 32 mL/L water; pH = 2.78; soluble solids = 1.4%). Crystal Clear contains no hormones and did not affect rate of
Rafael Urrea-López, Rocío I. Díaz de la Garza, and Juan I. Valiente-Banuet
186001344; Milford, MA) with an isocratic elution using a phosphate buffer (200 m m ; pH 2.4) as the mobile phase. Ascorbic acid quantification was estimated at 244 nm using calibration curves made with an ascorbic acid standard (Sigma-Aldrich, St. Louis, MO
John C. Beaulieu and Jeanne M. Lea
Instruments, Wilmington, DE) 5 min at 10,000 g n at 4 °C. Supernatant (3 mL) was removed to a test tube, to which 1 mL derivatizing solution, 1,2-benzenediamine dihydrochloride, was added (final Ph, 2.20–2.45), vortexed for 5 s, filtered through a 45-μm
Shijian Zhuang, Letizia Tozzini, Alan Green, Dana Acimovic, G. Stanley Howell, Simone D. Castellarin, and Paolo Sabbatini
± 0.05 g of homogenized sample was added to a tared 15-mL centrifuge tube and the mass was recorded. Ten milliliters of 50% v/v aqueous ethanol acidified to pH 2 (≈1 mL 12.1 M HCL) was added to the 1-g sample and then mixed once every 5 min manually
Cristián Vela-Hinojosa, Héctor B. Escalona-Buendía, José A. Mendoza-Espinoza, Juan M. Villa-Hernández, Ricardo Lobato-Ortíz, Juan E. Rodríguez-Pérez, and Laura J. Pérez-Flores
described by Nour et al. (2010) . Chromatographic separation was performed with an HPLC system (1260 Series; Agilent Technologies, Santa Clara, CA), using 50 m m potassium dihydrogen orthophosphate buffer, pH 2.8, as a mobile phase (flow rate of 0.7 mL
Shu Hsien Hung, Chun Chi Wang, Sergei Veselinov Ivanov, Vera Alexieva, and Chih Wen Yu
followed the procedure of Anderson et al. (1992) . Mung bean leaves (1 g fresh weight) were homogenized in 3 mL of ice-cold acidic extraction buffer [6% (w/v) meta-phosphoric acid (pH 2.8), containing 1 m m EDTA], using a Polytron homogenizer (model PT
John M. Dole, Zenaida Viloria, Frankie L. Fanelli, and William Fonteno
, trachelium, and zinnia stems. Control solutions. Cut stems were held in DI water (pH 3.1–4.2; EC 0 dS·m −1 ), DI water supplemented with 200 mg·L −1 8-HQC (pH 2.8–3.1; EC 0.12–0.15 dS·m −1 ), tap water (pH 6.3–7.1; EC 0.18–0.23 dS·m −1 ), or tap water with 8