The influence of genotype x environment interactions on the performance of `Autumn Bliss' `Heritage' and `Redwing' primocane-fruiting (PF) red raspberry (Rubus idaeus L.) cultivars was studied at six sites across Ontario and Quebec during 1989 and 1990. Cultivar × location × year interactions were found for most vegetative and reproductive components analyzed. `Autumn Bliss' had the most consistent performance of the three cultivars in all location/year combinations, while `Redwing' varied greatly between environments. `Heritage' was always the latest-bearing of the three cultivars and failed to achieve its maximum yield potential in many of the northern locations.
Jean-Pierre Privé, J.A. Sullivan, J.T.A. Proctor, and O.B. Allen
Shiow Y. Wang and Hsin-Shan Lin
Fruit and leaves from different cultivars of thornless blackberry (Rubus sp.), red raspberry (Rubus idaeus L.), black raspberry (Rubus occidentalis L.), and strawberry (Fragaria × ananassa D.) plants were analyzed for total antioxidant capacity (oxygen radical absorbance capacity, ORAC) and total phenolic content. In addition, fruit were analyzed for total anthocyanin content. Compared to fruit, leaves were found to have higher ORAC values. In fruit, ORAC values ranged from 7.8 to 33.7 μmol Trolox equivalents (TE)/g of fresh berries, while in leaves, ORAC values ranged from 20.8 to 45.6 μmol TE/g of fresh leaves. Fruit harvested at different stages of maturity were analyzed in blackberries, raspberries, and strawberries. Blackberries and strawberries had their highest ORAC values during the green stages, while raspberries generally had the highest ORAC activity at the ripe stage (with exception of cv. Jewel, a black raspberry). Total anthocyanin content increased with maturity for all three fruit. There was a linear correlation existed between total phenolic content and ORAC activity for fruit and leaves. For ripe berries, there was also a linear relationship between ORAC values and anthocyanin content. Of the ripe fruit and leaves tested, raspberry plants appeared to be the richest source for antioxidants.
JoAnn Robbins and Patrick P. Moore
Fruit weight and morphological characteristics of `Meeker' red raspberry (Rubus idaeus L.) fruit, including drupelets (height, diameter, number), receptacle cavities (depth, diameter), and pits (individual weight) were measured five times in 1988. Fruit strength, as measured by compression, was recorded. The relationship of fro-it weight to fruit strength had linear and quadratic components. Fruit weight was correlated with fruit strength, drupelet height and number, receptacle cavity depth and diameter, and individual pit weight. Besides fruit weight, fruit strength was correlated with drupelet diameter and number, receptacle cavity depth, and individual pit weight. Drupelet number, receptacle cavity depth, and individual pit weight provided the largest component contribution to fruit strength, as determined by path analysis.
Rita L. Hummel and Patrick P. Moore
Seasonal changes in freezing tolerance of stems and buds of Rubus idaeus L. `Chilliwack', `Comox', `Meeker', `Skeena' and `Willamette' clones were measured from November through March of 1988-1989 and 1989-1990. Eight additional clones were tested in 1989-1990. Canes were harvested from the field, cut into two-bud samples and subjected to controlled freezing tests. Samples were seeded with ice, held at -2°C overnight and then frozen at 3°C/hour. Viability was estimated by visual browning. Vascular tissue at the base of the buds was the least freeze tolerant tissue in these samples. Results of both the 1988-1989 and 1989-1990 freezing tests, indicated `Meeker' and `Willamette' cold acclimated more slowly in the fall than `Chilliwack', `Comox' and `Skeena'. However, in the spring, `Willamette' and `Meeker' were slower to lose freeze tolerance than the other three clones.
Pat Bowen and Stan Freyman
Raspberries (Rubus idaeus L.) were grown for 5 years with three floor management treatments: clean cultivation (CC) and ground covers of white clover (Trifolium repens L.) (WC) and perennial ryegrass (Lolium perenne L.) (PR). Primocane growth was strongest with WC and weakest with PR. Fruit production did not differ between WC and CC treatments and was higher than with PR. On 22 Aug. and 10 Sept. in the last year, primocanes grown with WC were taller, had more nodes and a higher dry weight, contained more N, and had retained more leaves than those grown with PR. Net CO2 assimilation per unit leaf area was higher with WC than with PR, and the difference was greater at the more proximal position. The estimated net CO2 assimilation rate per primocane was more than twice as high with WC than with PR.
David C. Percival, John T.A. Proctor, and J. Alan Sullivan
Field experiments consisting of trickle irrigation (TI), IRT-76 plastic film (PF), and straw mulch were initiated to determine the influence of soil temperature and water status on carbon partitioning during the establishment of Rubus idaeus L. `Heritage' (1993, 1994), `Autumn Bliss' (1994), and `Summit' (1994) micropropagated raspberries. Environmental, vegetative, reproductive, and nutrition data were collected. Photosynthesis (Pn) measurements were recorded under field conditions using a Li-Cor LI-6200 portable photosynthesis system. Neither node number nor shoot: root ratio was influenced by TI, PF, or straw mulch. PF, however, increased root and shoot weight, total flowers produced, total berries harvested, and foliar N and P. Although differences existed among cultivars, field Pn measurements indicated that, regardless of groundcover treatment or cultivar examined, the maximum Pn rate occurred at a root-zone temperature of 25C. Hence, results from this study indicate that conditions in both the air and root zone physical environment regulate carbon assimilation and partitioning.
J.A. Sullivan, B.A. Hale, and D.P. Ormrod
Factorial experiments in two growing seasons in open-top field chambers with two or three O3 concentrations and two primocane-fruiting raspberry (Rubus idaeus L.) cultivars were used to obtain dose-response relationships describing the effects of seasonal O3 exposure on raspberry plant vegetative and reproductive growth. At the lower concentration (0.12 μl·liter-1), the response to O3 was nonsignificant. However, at 0.24 μl·liter-1, `Heritage' showed a significant decline relative to the control in cane height, node count, cane diameter, and dry weight. These changes were accompanied by a 52% decrease in yield, caused mainly by a reduction in fruit count. In contrast, vegetative and yield characters of the `Redwing' were not affected by O3.
Pauliina Palonen, Danielle Donnelly, and Deborah Buszard
Low tissue-water content and increased osmotic concentration of cell sap are associated with frost resistance. Changes in total osmotic concentration of cell sap are due mainly to changes in concentration of sugars. Generally, sugar content increases with hardening and decreases with dehardening. This study examined the effect of elevated sucrose levels (3% to 15%) in the medium on the cold hardiness of `Festival' red raspberry (Rubus idaeus L.) shoots in vitro. To determine whether expected hardening is caused by elevated sucrose levels or by osmotic stress, different levels of mannitol in the media have been tested. After growing raspberry shoots on media with different levels of sucrose and mannitol for 2 weeks, shoot moisture content (percent) was determined. Cold hardiness of the shoots was determined by using differential thermal analysis or artificially freezing the shoots and assessing the survival by regrowth test and visual rating.
M.A. Ellis and S.A. Miller
A commercially available serological assay kit (flow-through enzyme-linked immunosorbent assay, Phytophthora F kit) was compared to a culture-plate method for detecting Phytophthora spp. in apparently diseased (phytophthora root rot) and apparently healthy red raspberry (Rubus idaeus subsp. strigosus Michx.) plants. During 4 years of testing, 46 tests were conducted on apparently diseased roots. All diseased plants gave a strong positive reaction, a result indicating that Phytophthora spp. were present. Of the 46 plants that tested positive, Phytophthora spp. were recovered from all but one using a selective medium for Phytophthora and the culture-plate method. When the same test was conducted on 27 apparently healthy plants, all had a negative reaction for the presence of Phytophthora except one sample, which had a slight positive reaction. No Phytophthora spp. were isolated from any apparently healthy plants. Our results indicate that the serological test kit enables rapid, dependable, on-site diagnosis of raspberry phytophthora root rot.
Penelope Perkins-Veazie and Gail Nonnecke
Raspberry (Rubus idaeus L., `Heritage') fruit were harvested at six stages of color development to determine the relationship between quality attributes and physiological changes during ripening. Soluble solids concentration and fruit weight increased, whereas titratable acidity decreased during ripening. Fruit darkened and color saturation increased with maturity. Raspberry fruit exhibited a nonclimacteric pattern of respiration, and ethylene (C2H4) was detected only after red pigment developed. Respiration and C2H4 production of whole fruit were similar to those of drupes. Ethylene-forming enzyme activity commenced in drupes and receptacle tissue from fruit at the yellow and mottled stages, respectively. These data indicate that ripening in raspberry fruit is independent of C2H4 production and is nonclimacteric.