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Silvia R. Marino, Jeffrey G. Williamson, James W. Olmstead, and Philip F. Harmon

potential to produce large numbers of plants more quickly than by rooted cuttings, and tissue culture plants can be produced throughout the year ( Isutsa and Pritts, 1994 ; Miller et al., 2004 ). Although changes in the growth habit of micropropagated

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Yun-Peng Zhong, Zhi Li, Dan-Feng Bai, Xiu-Juan Qi, Jin-Yong Chen, Cui-Guo Wei, Miao-Miao Lin, and Jin-Bao Fang

screen potential resistant resources for kiwifruit breeding or rootstock selection in future studies, the growth and physiological responses of five Actinidia species to PEG-induced drought stress under tissue culture conditions were measured, and the

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Bo-Ling Liu, Zhi-Bin Fan, Ze-Qun Liu, Xun-Hong Qiu, and Yan-Hong Jiang

-pollination in the field may lead to varietal loss ( Shan et al., 2007 ). In recent years, methodological advances in plant tissue culture methods have allowed its use as a viable approach for multiplication, germplasm conservation, and genetic manipulation of

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Ute Albrecht, Shahrzad Bodaghi, Bo Meyering, and Kim D. Bowman

cuttings and tissue culture propagation, are required to produce the desired quantities of trees. Propagation by cuttings and tissue culture will result in genetically identical rootstocks that can be used as liners for grafting ( Albrecht et al., 2017a

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Muhammad Irshad, Hafiz Muhammad Rizwan, Biswojit Debnath, Muhammad Anwar, Min Li, Shuang Liu, Bizhu He, and Dongliang Qiu

tissue culture system among the chief reasons. There have been several published reports on okra regeneration because it was first successfully accomplished by Mangat and Roy (1986) using nodes and shoot tips of in vitro grown seedlings ( Anisuzzaman et

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Justin A. Schulze, Jason D. Lattier, and Ryan N. Contreras

(12 weeks after pollination) Prunus lusitanica seeds without testa in Expt. I. Seeds underwent 10-week cold stratification and 5 weeks under standard culture conditions in eight variations of MS ( Murashige and Skoog, 1962 ) tissue culture medium

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Allison D. Oakes, Tyler Desmarais, William A. Powell, and Charles A. Maynard

mycorrhizae formation ( D’Amico et al., 2014 ). Wild-type american chestnuts derived from tissue culture are important controls and are also needed in large numbers. Producing hardwood trees from tissue culture is an extremely time- and labor-intensive process

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Xiuli Shen and Myeong-Je Cho

of cold stratification, chemical scarification, GA 3 treatment, and artificial opening on seed germination. The results of this study can be applied to establish a tissue culture and genetic transformation protocol for sugar pine. Materials and

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Jonathan D. Mahoney and Mark H. Brand

multiplication cultures have been maintained for more than 4 years by subculturing every other month. There have been no symptoms of hybrid necrosis during this process. Both hybrids produce an adequate number of shoots in tissue culture; however, AA15-3 shoots

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Rashid Sultan Al-Obeed, Salah El-Hendawy, Mayada Kadri Seliem, and Khadija Rabeh Al-Harbi

commercial practice. An alternative approach is to propagate and conserve D. ombet through tissue culture techniques. Tissue culture facilitates the rapid production of propagules from several species that are difficult to propagate using conventional