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James C. Fulton, Francisco O. Holguin, Robert L. Steiner, and Mark E. Uchanski

) was followed for HPLC. During the first year, 6 mg of tissue per sample was extracted; the following year, 25 mg per sample was used. Samples were saponified with 1 mL of 2 N methanolic potassium hydroxide, vortexed for 30 s, and heated at 50 °C for 1

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Panthip Boonsong, Natta Laohakunjit, Orapin Kerdchoechuen, and Frank B. Matta

plants using ultraviolet-visible spectrophotometry and HPLC. This research will provide information on the presence, color, and number of pigments and polyphenols (colorants) in plant extracts for possible use as hair coloring dyes. Materials and Methods

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Yifei Wang, Stephanie K. Fong, Ajay P. Singh, Nicholi Vorsa, and Jennifer Johnson-Cicalese

V. tenellum . By using HPLC and LC-ESI-MS-MS, the objectives of the study were to investigate 1) flavonoid (anthocyanins, flavonols, and proanthocyanidins) and organic acid profiles of different blueberry species, 2) variation of flavonoid and

Open access

Calen McKenzie, Ivette Guzman, Ciro Velasco-Cruz, and Paul W. Bosland

., 2012 ). About 1.0 g of fresh leaf tissue from each sample was ground in a mortar and pestle with 15 mL of reagent high-performance liquid chromatography (HPLC)-grade ethanol (Sigma-Aldrich, St. Louis, MO) as the extraction solvent. The ethanolic leaf

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Cheng Bai, Charles C. Reilly, and Bruce W. Wood

HPLC analysis of xylem sap collected at the time of bud break. Bud break is defined here as the inner bud scale split of >50% of primary apical buds. Spring xylem sap was collected and analyzed at the same stage of bud break from trees reflecting two

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Claire H. Luby, Rachael Vernon, Hiroshi A. Maeda, and Irwin L. Goldman

-tocopherol and α, β-, and γ-tocotrienol. Burns et al. (2003) and Koch and Goldman (2005) used reverse-phase HPLC equipped with an ultraviolet detector to quantify vitamin E levels in carrot roots. These studies reported values of 0.03–0.11 µg·g −1 of total α

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Patrick J. Conner and Dan MacLean

. Two notable exceptions were ‘Tarheel’ and ‘Noble’, which contained good amounts of malvidin and petunidin and produced wines of acceptable color. Since that early work, several authors have used HPLC to better examine the anthocyanin profile of

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Ting Lei, Yang Song, Xuehua Jin, Tianyu Su, and Yiwen Pu

measured using HPLC ( Li et al., 2008 ). TA was measured using an Agilent HPLC equipped with a P680 pump, UltiMate 3000 autosampler, DAD-100 ultraviolet-visible (ultraviolet-vis) detector, TCC-100 column oven and Agilent ZORBAX SB-Aq (4.6 mm × 250 mm

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Au Trung Vo, Imane Haddidi, Hussein Daood, Zoltan Mayer, and Katalin Posta

, then filtered through a filter paper. It was further cleaned-up by passing through a 0.22 µm PTFE HPLC syringe filter before injection on to the HPLC column for the analysis of polyphenols. We used Nucleosil C18, 100, Protect-1 (Macherey-Nagel, Duren

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Xiaohua Yang, Susan K. Brown, and Peter J. Davies

extract by Halińska et al. (1989) and purified by solid phase extraction (Strata-X SPE; Phenomenex, Torrance, CA) and high-performance liquid chromatography (HPLC) ( Davies et al., 1986 ). The [ 14 C]GA 12 contained eight 14 C atoms per GA 12 molecule