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Fuad Gasi, Naris Pojskić, Mirsad Kurtovic, Clive Kaiser, Stein Harald Hjeltnes, Milica Fotiric-Aksic, and Mekjell Meland

unreduced gametes from one or both parents) ( Phillips et al., 2016 ). Microsatellite markers or simple sequence repeats (SSRs) have proven efficient in parent-offspring analyses on pear ( Kimura et al., 2003 ). Although a comparative study has shown that

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Fuad Gasi, Silvio Simon, Naris Pojskic, Mirsad Kurtovic, Ivan Pejic, Mekjell Meland, and Clive Kaiser

these genetic resources ( Hokansson et al., 1998 ). Examining ex situ collections with microsatellite markers has so far been performed in a number of studies ( Garkava-Gustavsson et al., 2008 ; Gharghani et al., 2009 ; Guarino et al., 2006 ; Guilford

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Michael Dossett, Jill M. Bushakra, Barbara Gilmore, Carol A. Koch, Chaim Kempler, Chad E. Finn, and Nahla V. Bassil

filter was applied to identify reads with microsatellites located near the center of the sequence. This filter yielded the longest possible flanking sequences for subsequent primer design ( Jennings et al., 2011 ). The program cd-hit-454 ( Niu et al

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Megan F. Muehlbauer, Josh A. Honig, John M. Capik, Jennifer N. Vaiciunas, and Thomas J. Molnar

-term breeding efforts to develop durable resistance in this long-lived, perennial species. Fortunately, molecular tools are now available to characterize hazelnut germplasm. Microsatellite, or simple sequence repeat (SSR), markers are particularly valuable for

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María José Arismendi, Patricio Hinrichsen, Ruben Almada, Paula Pimentel, Manuel Pinto, and Boris Sagredo

; Rojas et al., 2008 ). These have been widely used to characterize and classify commercial cultivars or estimate the genetic relationships among members of this genus. Among these, the microsatellite (SSR) markers are preferred because of their high level

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Theodore J. Kisha and Christopher S. Cramer

. Additionally, analysis of worldwide genetic diversity can identify areas suited for the establishment of in situ conservation sites ( Greene et al., 2008 ). Microsatellite markers, or simple sequence repeats [SSRs ( Oliveira et al., 2006 ; Tautz and Renz, 1984

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Carlos Miranda, Jorge Urrestarazu, Luis G. Santesteban, José B. Royo, and Valero Urbina

research program was launched to evaluate the genetic diversity of the collection through detailed morphological and agronomical description, and fingerprinting analysis based upon molecular markers was initiated in 2005. Microsatellite or simple sequence

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L.J. Grauke, Muhammad J. Iqbal, Avutu S. Reddy, and Tommy E. Thompson

A microsatellite-enriched library was developed from `Halbert', a native pecan [Carya illinoinensis (Wangenh.) K. Koch] selection from Coleman County, Texas. A genomic library enriched for simple sequence repeats (SSR) containing 6144 clones was archived in 384 well plates for screening. In total, 439 clones were identified after Southern hybridization using di- and tri-nucleotide repeats as probes. In total, 125 positive clones were sequenced and primers were designed for 24 repeats. The SSR markers chosen for analysis include di-(CT and GA) and tri-nucleotide repeats (CTT, GAA and GAT). Of the 24 primer pairs tested, 19 successfully amplified microsatellites from `Halbert'. DNA was isolated from 48 pecan and hickory accessions selected to strategically represent the genetic diversity of the National Clonal Germplasm Repository (NCGR) Carya collections. The 19 SSR primers that produced good amplification products in `Halbert' were used to evaluate the collection, with 11 revealing polymorphism. The number of fragments amplified with different primer combinations ranged from 4 to 32 in the 48 genotypes tested. Evaluation of the data confirms the utility of the microsatellites in delimiting known relationships.

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Yiqun Weng, Shanna Johnson, Jack E. Staub, and Sanwen Huang

and Staub (1999 ; used RAPD markers). Moreover, the microsatellite markers used here were developed from a whole genome sequence of cucumber inbred line 9930 through pre-selection of long repeat motifs ( Ren et al., 2009 ), which are generally more

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Eder J. Oliveira, Maria Lucia C. Vieira, Antonio Augusto F. Garcia, Carla F. Munhoz, Gabriel R.A. Margarido, Luciano Consoli, Frederico P. Matta, Michel C. Moraes, Maria I. Zucchi, and Maria Helena P. Fungaro

codominant markers, particularly microsatellite loci. To our knowledge, this is the first integrated genetic map of a passion fruit species. Materials and Methods Plant materials, extraction, and quantification of DNA. Carneiro et al. (2002