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Mary Lewis, Matthew Chappell, Donglin Zhang, and Rebekah Maynard

and Pearson, 1948 ; Whiting, 1943 ). Embryo abortion is difficult to overcome; however, by prematurely harvesting seeds, removing the embryo, and aseptically culturing the embryo on nutrient medium, pod abortion and developmental failure could be

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Guochen K. Png, Katherine S. Downes, and Beng H. Tan

sinensis Plant Cell Tissue Organ Cult. 35 173 179 Nikolaeva, M.G. 2004 On criteria to use in studies of seed evolution Seed Sci. Res. 14 315 320 Nissen, S.J. Sutter, E.G. 1990 Stability of IAA and IBA in nutrient medium to several tissue culture procedures

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Ying Liu, Huawei Song, Juming Zhang, and Michael D. Richardson

Root lengths. Seedlings grown in nutrient medium supplemented with various concentrations of B did not show any difference in root lengths compared with controls at any of the three treatment times (2, 4, and 6 d; Fig. 1A ). However, the addition of Ca

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Aekaterini N. Martini, Maria Papafotiou, and Stavros N. Vemmos

pathogens overwinter inside the buds, whereas they infect other organs during spring and summer ( Holb et al., 2004 ). Moreover, bud surface sterilization was most probably hindered by the dense hairs of × M. florentina buds. Seeds placed on nutrient medium

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Allison D. Oakes, Tyler Desmarais, William A. Powell, and Charles A. Maynard

, E. 1990 Stability of IAA and IBA in nutrient medium to several tissue culture procedures. HortScience 25:800–802 Oakes, A.D. Powell, W.A. Maynard, C.A. 2013 Doubling acclimatization survival of micropropagated american chestnuts with darkness and

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Sameer Pokhrel, Bo Meyering, Kim D. Bowman, and Ute Albrecht

). Disinfected embryos were placed into clear polypropylene containers containing Murashige and Skoog (MS) agar-nutrient medium ( Murashige and Skoog, 1962 ) without added growth regulators. Embryos were dissected after pre-germination of seeds and identified as

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Arthur Villordon and Jeffrey C. Gregorie

solution ( Hoagland and Arnon, 1950 ). Three B levels were used: 0.1 mg·kg −1 (0B; B was omitted in the nutrient medium, substrate B = 0.1 mg·kg −1 as shown in Supplemental Table 1 ), 0.5 mg·kg −1 (1XB; sufficient B, control) and 1 mg·kg −1 (2XB, high