, and the combination of ABA plus BA resulted in significant thinning when expressed either as fruit per centimeter LCSA or as percent set ( Table 1 ). The interaction between ABA and BA for fruit abscission was significant. ABA, BA, and the combination
Duane W. Greene, James R. Schupp, and H. Edwin Winzeler
Hakan Aktas, Derya Bayındır, Tuba Dilmaçünal, and M. Ali Koyuncu
). Chemicals such as 1-methylcyclopropane ( Beno-Moualem et al., 2004 ), silver thiosulfate, and aminoethoxyvinylgycine block the production and action of ethylene and thereby inhibit tomato fruit abscission ( Lichter et al., 2006 ). Salicylic acid can cause
Steven McArtney, John Palmer, Sue Davies, and Shona Seymour
The effect of liquid lime sulfur (LS) and fish oil (FO) application during bloom on leaf photosynthesis (Pn) and pollen tube growth in apple (Malus ×domestica) flowers were investigated in order to determine their mode of action as a bloom thinning agent. LS increased the percentage of flowers with fewer than 10 pollen tubes per flower to more than 64% compared to 5% or less in the control. Pollen tubes were completely absent from 27% to 48% of flowers following LS treatment compared with fewer than 4% of flowers having no pollen tubes on control trees. These data indicate that 30% to 50% of flowers that open on the day of LS application are unlikely to set a fruit due to the complete inhibition of embryo fertilization. Increasing the rate of LS from 0.5% to 4% increased the proportion of flowers with limited pollen tube number in a concentration dependent manner. LS suppressed the rate of light saturated Pn; successive LS sprays during the bloom period had an additive effect on suppression of Pn and fruit set. In one study the reduction in Pn was greatest 12 days after application of LS but Pn recovered by about 19 days after initial treatment. In a second study Pn of primary spur leaves had still not recovered when measured 57 days after the first of three applications. FO had no effect on the number of pollen tubes per flower, but reduced Pn and fruit set by about 10% and 20% respectively. An increase in the proportion of flowers with no pollen tubes, and therefore no embryo development, can account, at least in part, for the thinning response following application of LS to apples during bloom. It is likely that suppression of Pn contributes to the thinning response, although the importance of this mechanism will depend on perturbation of the total carbohydrate supply to developing fruit.
Tjasa Burnik-Tiefengraber, Kitren G. Weis, Barbara D. Webster, George C. Martin, and Hisashi Yamada
When continuously stem-fed with 75 mm NaH2PO4, `Manzanillo' olive explants showed significant leaf abscission after 48 hours; by that time 1.042 mg·g-1 fresh weight P had accumulated in the abscission zone (AZ). The potential contribution of ethylene to phosphate-enhanced abscission was investigated using aminooxyacetic acid (AOA), an ethylene-synthesis inhibitor, and by measuring ethylene evolution in phosphate-treated explants. In combination with NaH2PO4, AOA did not affect leaf abscission. Though ethylene evolution from explants increased as leaf abscission was initiated, it was about two orders of magnitude less than the concentration necessary to induce leaf abscission as judged by exogenous treatments. Based on leaf-abscission kinetics, we have concluded that the mechanism of P-induced abscission is independent of gross measurement of evolved ethylene, but we cannot rule out ethylene confined to the AZ itself. When evaluated for P-induced leaf abscission, leaves of `Manzanillo' and `Sevillano' abscised earlier than `Ascolano' and `Mission'.
L. Rallo, G. C. Martin, and S. Lavee
Differences in pistil abortion did not account for variation in fruiting among ‘Manzanillo’ (biennal bearing), ‘Rubra’ (regular cropping), and ‘Swan Hill’ (strong blooming but unfruitful) olives (Olea europaea L.). Competition among normal fruits in an inflorescence seems to be a main factor in regulating final crops. Parthenocarpic fruit of ’Manzanillo’ and ‘Swan Hill’ did not exhibit fruit competition. A high percentage of ovules in ‘Swan Hill’ contained poorly developed embryo sacs at anthesis and were not fertilized. Most of the few parthenocarpic fruit formed eventually shriveled and abscised.
A. A. Powell and A. H. Krezdorn
Self-pollination, emasculation and gibberellic acid (GA) were used to study translocation patterns of l4C-metabolites during flowering and fruiting in calamondin (Citrus madurensis Lour.). Radioautographs showed similar translocation patterns with self-pollination and GA. GA and self-pollination resulted in a considerably stronger mobilization of 14C-metabolites to young ovaries and developing fruits than when flowers were emasculated and no further stimulus provided. The movement of l4C-metabolites to fruits, especially in the 3-week period after anthesis, appeared essential for fruit set and development.
Raquel Cano-Medrano and Rebecca L. Darnell
To determine if multiple applications of GA3 would increase size of parthenocarpic fruit, and to assess the interaction between GA3 applications and pollination, `Beckyblue' rabbiteye blueberry (Vaccinium ashei Reade) flowers were treated with single or multiple applications of GA3 alone or in combination with full or partial pollination. Single or multiple applications of GA3 resulted in similar or increased fruit set compared with pollination, and increased fruit set compared with no pollination. GA3 applications decreased fruit mass and increased the fruit development period in comparison with pollination alone. Multiple, late applications of GA3 were ineffective in overcoming these effects. Partial (nonsaturating) pollination resulted in an average fruit set of 60%, while set following GA3 treatment in combination with full or partial pollination averaged 85%. Fruit mass was greater in the full pollination ±GA3 treatments than in all other treatments. The number of large seeds and seed mass per fruit were greatest in the full pollination treatment, and were significantly decreased by all treatments in which GA3 and/or partial pollination were used; however, there were no concomitant effects of GA3 in delaying the fruit development period. Our results indicate that under optimal pollination conditions, no detrimental effects of GA3 applications on fruit set, fruit size, or fruit development period in blueberry are to be expected, even though GA3 reduces seed number and seed mass. Furthermore, GA3 applications appear to be beneficial in increasing fruit set under suboptimal pollination conditions, although smaller fruit are to be expected under such conditions. Chemical name used: gibberellic acid (GA3).
Rongcai Yuan, Ulrich Hartmond, Angela Grant, and Walter J. Kender
Influence of young fruit, shoot, and root growth on response of mature `Valencia' oranges [Citrus sinensis (L.) Osbeck] to the abscission chemical CMN-pyrazole was examined in 1999 and 2000. CMN-pyrazole dramatically increased ethylene production in mature fruit and reduced the fruit detachment force (FDF), except during a period of reduced response to CMN-pyrazole in early May when spring vegetative growth, young fruit of the following year's crop, and mature fruit were all on the trees. Removal of spring flushes, which included spring vegetative shoots and leafy and leafless inflorescences, prevented any young fruit and shoot growth, but did not inhibit root growth. However, trunk girdling in combination with removal of spring flushes not only prevented growth of young fruit and shoots but also inhibited root growth. During the responsive period, there were no differences in either ethylene production or FDF of CMN-pyrazole-treated mature oranges between 1) the nonmanipulated trees and those manipulated by either 2) removal of spring flushes alone, or 3) in combination with trunk girdling. However, during the less responsive period, ethylene production in CMN-pyrazole-treated mature oranges was significantly lower while the FDF was higher in nonmanipulated trees than in trees treated by either removal of spring flushes alone, or in combination with trunk girdling. There was no difference in either fruit ethylene production or FDF between trees manipulated by (2) removal of spring flushes alone, and (3) removal of spring flushes in combination with trunk girdling regardless of CMN-pyrazole application. Shoot growth terminated at least 2 weeks before the onset of the less responsive period. Removal of young fruit increased response of mature fruit to CMN-pyrazole during the less responsive period. This suggests that hormones from rapidly growing young fruit may be responsible for the occurrence of the less responsive period. Chemical name used: 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMN-pyrazole).
Hong Zhu, Rongcai Yuan, Duane W. Greene, and Eric P. Beers
Ethylene is involved in young apple fruit abscission ( Curry, 1991 ; Dal Cin et al., 2005 ; McArtney, 2002 ; Zhu et al., 2008 ). Application of ethephon, an ethylene-releasing compound, effectively promoted the abscission of young fruit in
Rongcai Yuan and Jacqueline K. Burns
The effect of temperature on the ability of 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMNP) and ethephon to induce ethylene evolution and abscission of mature fruit and leaves was determined using 3-year-old potted `Hamlin' orange [Citrus sinensis (L.) Osb.] trees in environment-controlled growth rooms in seasons 2001-02 and 2002-03. Ethylene evolution and abscission of CMNP or ethephon-treated fruit and ethephon-treated leaves were highly temperature dependent. Fruit detachment force (FDF) and fruit ethylene evolution were not affected by application of ethephon at 200 mg·L-1 or CMNP at 200 mg·L-1 when air temperature was 10 °C for ethephon treatment or ≤15.6 °C for CMNP treatment. However, ethylene evolution of CMNP or ethephon-treated fruit increased sharply, and FDF decreased drastically as temperature increased from 10 to 26.7 °C for ethephon treatment or from 15.6 to 26.7 °C for CMNP treatment. Several 10 hour day/14 hour night temperature regimes were explored to determine the effect of varying daily and nightly temperatures on efficacy and ethylene evolution. At least 3 days of exposure to 21/10 °C were required for CMNP to effectively loosen fruit, whereas only one day of exposure to 26.7/15.6 °C was enough to induce similar changes. At 21/10 °C, CMNP significantly reduced FDF to<25 N and markedly enhanced fruit ethylene evolution, regardless of interruption by 1 day of low temperature at 10/10 °C in the first 5 d after application. Ethephon had no significant effect on leaf ethylene evolution and leaf abscission when temperature was 10 °C, but caused a marked increase in both leaf ethylene evolution and leaf abscission as temperature increased from 10 to 26.7 °C. CMNP did not stimulate leaf ethylene evolution and leaf abscission regardless of temperature. Chemical names used: 5-chloro-3-methyl-4-nitro-1 H-Pyrazole (CMNP); 2-chloroethylphosphonic acid (ethephon).