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J. Adelberg, M. Kroggel, and J. Toler

Hosta ×hybrid Tratt. `Blue Cadet' and Hosta tokudama Tratt. `Newberry Gold' were micropropagated in shaken liquid culture and on agar media, in conventional vessels and vessels modified for ventilation in vitro. Acclimatization under intermittent mist and growth in an outdoor nursery during the late spring and summer were monitored by dry weight analysis of sample plants every 4 days for a 60-day period (ex vitro growth). Results for `Newberry Gold' were 1) in vitro shoot growth was greater in liquid than agar culture, regardless of vessel; 2) shoots from agar or liquid culture grew at similar rates ex vitro; 3) ex vitro root growth was greater for liquid than agar cultured plants, regardless of vessel type. Results for `Blue Cadet' were 1) in vitro and ex vitro shoot growth was greater in liquid than agar culture regardless of vessel type and 2) ex vitro root growth was greatest for liquid cultured plants from conventional vessels. Ventilated vessels were generally beneficial for agar but not liquid culture. Benefits of liquid culture for micropropagation of Hosta found in vitro are at least maintained and sometimes enhanced during ex vitro growth in the mist bed and nursery.

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Rashid Sultan Al-Obeed, Salah El-Hendawy, Mayada Kadri Seliem, and Khadija Rabeh Al-Harbi

dependent on medium strength and the type and concentration of auxins. Regenerated plantlets of D. ombet were acclimatized to ex vitro conditions ( Fig. 2C and D ), and a 95% survival rate for the plantlets was achieved after 8 weeks. Table 2. Effect of MS

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Renee Timmermann and M.A.L. Smith

The structure and quality of the adventitious root system formed on a microcutting stem is crucial to the successful acclimation, survival, and ultimate performance of micropropagated plants. Despite increasing evidence that the rooting method impacts on the character of the framework root system, very little research has elucidated the consequences of standard rooting methods on plant quality and performance. Root initiation on microcuttings is handled by a wide range of strategies in commercial practice. In comparison to in vitro roots, ex vitro roots have smaller root diameters, larger vascular diameters, greater length, more branch root development, and root hairs. One important microenvironmental parameter - light - was investigated to determine its contribution to root character. Typically, in vitro root systems are exposed to light throughout the root initiation period. Parallel treatments were established of in vitro light and dark rooting and ex vitro rooting of four woody species. Regardless of light exposure, the overall diameter of adventitious roots was larger for in vitro treatments than the ex vitro treatment. Vascular development was significantly more advanced ex vitro. These results suggest that light is not a major influence contributing to the differences between ex vitro and in vitro root character.

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Maria Papafotiou and Aekaterini N. Martini

ex vitro axillary buds ( Al-Qudah et al., 2011 ) and cryopreservation ( Rabba’a et al., 2012 ). It has also been reported that micropropagation of the ornamental medicinal Teucrium fruticans from shoot-tip explants ( Frabetti et al., 2009 ) and of

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Toktam Taghavi, Alireza Rahemi, Reza Rafie, and Maru K. Kering

) and Driver and Kuniyuki walnut medium (PhytoTech Laboratories, Lenexa, KS). (B) This jar produced 12 plantlets (with roots), and the tallest plant was 167 mm. (C) Three plants of each treatment were transferred to ex vitro conditions; 1 mm = 0

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Carmen Valero Aracama*, Michael E. Kane, Nancy L. Philman, and Sandra B. Wilson

A sea oats (Uniola paniculata L.) micropropagation protocol was previously developed for 28 genotypes that favored multiplication and rooting of shoots in vitro. However, microcutting size, morphology, and acclimatization ex vitro varied considerably among genotypes. In the present study we evaluated the effect of Stage III duration on in vitro morphology, biomass production, and ex vitro survivability of easy-(EK 16-3) and difficult-to-acclimatize (EK 11-1) sea oats genotypes. After 3, 6, and 9 weeks at Stage III, survivability of microcuttings was 85%, 96% and 98% for EK 16-3, and 2%, 27% and 40% for EK 11-1, respectively. After 9 weeks Stage III, EK 16-3 microcuttings had higher shoot dry weights but lower root dry weights than in EK 11-1. Moreover, roots in EK 11-1 were fewer but longer than in EK 16-3. Leaf production was similar in both genotypes. However, leaf elongation was significantly inhibited in EK 11-1, in which 95% of the leaves were ≤ 15 mm long in contrast with EK 16-3, with 50% leaves ≥ 16 mm long after 9 weeks Stage III. Light microscopy examinations showed anatomical similarities between EK 16-3 in vitro leaves and mature ex vitro leaves. Conversely, short in vitro leaves of EK 11-1 exhibited mesophyll disruption and reduced cuticle development. Conceivably, the short leaves had limited photosynthetic competency, thereby reducing ex vitro survival of rooted EK 11-1 microcuttings.

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Jeffrey Adelberg, Maria Delgado, and Jeffrey Tomkins

total of 6 weeks ex vitro, survival and whole plant FW was determined. The experiment was a completely randomized design with four genotypes, two BA concentrations, two ANC concentrations, two sucrose concentrations, and two explant densities (4 × 2

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Lauren E. Deans, Irene E. Palmer, Darren H. Touchell, and Thomas G. Ranney

), and H. serrata ‘MAK20’ ( C ) plants during their third ex vitro growing season. A comparison of floral characteristics showed that induced tetraploidy caused a significant decrease in inflorescence number for all cultivars ( P < 0.05), with an

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John L. Edson, David L. Wenny, and Annette Leege-Brusven

In vitro—derived microshoots of antelope bitterbrush, incubated for 1 month in media supplemented with 0.44 μm BA, grew 0.8 and 1.1 cm longer in woody plant medium (WPM) compared to full-strength and half-strength Murashige and Skoog (MS) media, respectively. Explants cultured in WPM supplemented with 0.44 μm BA and 0.54 μm NAA produced a mean of five axillary shoots per explant. Explants dipped in 0.1% IBA or 0.1% NAA rooted best in 0.1% IBA with 89% success ex vitro vs. 60% success in vitro. Survival of acclimatized plantlets rooted ex vitro was 95%, while 50% survived when rooted in vitro. After 1 year of greenhouse growth, 98% of plantlets survived and flowered. Chemical names used: benzyladenine (BA), 3-indolebutyric acid (IBA), 1-naphthaleneacetic acid (NAA).

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Yulan Xiao and Toyoki Kozai

A photoautotrophic or sugar-free medium micropropagation system (PAM) using five large culture vessels (volume = 120 L each) with a forced ventilation unit for supplying CO2-enriched air was developed and applied to commercial production of calla lily (Zantedeschia elliottiana) and china fir (Cunninghamia lanceolata) plantlets. The culture period of calla lily plantlets in the PAM was reduced by 50%, compared with that in a conventional, photomixotrophic micropropagation system (PMM) using small vessels each containing a sugar-containing medium. Percent survival ex vitro of calla lily plantlets from the PAM was 95%, while that from the PMM was 60%. The production cost of calla lily in the PAM was reduced by about 40%, compared with that in the PMM, and the initial investment per plantlet for the PAM was ≈10% lower than that for the PMM. The sales price of ex vitro acclimatized calla lily plantlet was increased by 25% due to its higher quality, compared with plantlets produced in the PMM.