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Yin-Tung Wang

Lilium longiflorum Thunb. `Nellie White' plants were selected when their first flower buds reached 2 or 5 cm in length, sprayed with 2 mL of PBA at 0 or 500 mg·L–1, and then placed under 1440 or 60 μmol·m–2·s–1 photosynthetic photon flux (PPF) during flowering. PBA resulted in delayed anthesis and increased dry matter accumulation in flowers under the high PPF but had no effect under the low PPF. PBA did not decrease the severity of flower bud abortion under the low PPF. Application of PBA induced the formation of numerous bulbils in the leaf axils. Regardless of PPF, PBA-treated plants had less dry weight in the main bulbs than the control plants. Chemical name used: N-(phenylmethyl)-9-(tetra-hydro-2H-pyran-2-yl)-9H-purin-6-amine (PBA).

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Sven E. Svenson

Rooting and growth of Verbena cuttings (Verbena × hybrids Voss) were measured to determine response to foliar-applied benzylaminopurine (BA). There was no rooting response to BA application when visible nodal roots were present at the base of the cutting. There was no response to 30, 100, or 300 mg BA/liter applied to the foliage 48 or 96 hours after excision from the stock plant. Rooting-zone dry mass, total cutting dry mass, and number of roots were increased by 30 mg BA/liter applied immediately after excision when there were no visible nodal roots at the base of the cuttings. Foliar application of BA at 10 or 30 mg·liter-1 increased lateral bud elongation of subsequently rooted shoots by 20% and 49%, respectively. Application of BA during cutting propagation to enhance subsequent lateral bud elongation does not appear to inhibit rooting in Verbena stem cuttings. Chemical name used: 6-benzylaminopurine (BA).

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Eric A. Curry and Duane W. Greene

CPPU was applied to whole spur `Delicious' apple (Malus domestica Borkh.) trees in central Washington at 0,6.25,12.5,25, or 50 mg·liter-1 at full bloom (FB) or FB plus 2 weeks. At both application times, the flesh firmness of treated fruit linearly increased with increasing concentration. CPPU applied at 0,5,10,15, or 20 mg·liter-1 to spur `Delicious' trees in Massachusetts at king bloom resulted in a linear increase in flesh firmness at harvest and following 28 weeks in air storage at 0C. CPPU did not affect the incidence of senescent breakdown, decay, or cork spot. Fruit length: diameter (L/D) ratios generally increased at all doses. Fruit weight was not influenced at either location. All CPPU concentrations reduced return bloom on `Delicious' apples in Massachusetts in 1989. Of the 10, 20, or 40 mg·liter-1 treatments for `Empire' apples, only CPPU at 40 mg·liter-1 reduced return bloom. CPPU applied to `Empire' apples in Massachusetts did not effect fruit set, soluble solids concentration, L/D, or firmness; however, fruit weight increased linearly with concentration. CPPU applied at 100 mg·liter-1 retarded preharvest fruit drop of `Early McIntosh' in Massachusetts for ≈7 days but was not as effective as NAA at 20 mg·liter-1. In a larger semicommercial trial, `Delicious' fruit treated with CPPU at 5,10, or 15 mg·liter-1 at FB, petal fall (PF), or PF plus 1 week, respectively, were harvested and graded over a commercial packing line. Malformities caused by CPPU at the highest doses reduced packout, although all CPPU application rates reduced the percent fruit culled due to poor color. CPPU increased packed fruit size, since the size of fruit (64 mm in diameter) in the >150-fruit/box size decreased, while the size of fruit (72 mm in diameter) in the 100- and 130-fruit/box sizes increased. Treated fruit stored for 7 months at 1C were firmer than nontreated controls. Chemical names used: N-(2-chloro-4-pyridyl)- N' -phenylurea (CPPU); 1 naphthalene-acetic acid (NAA).

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Sauveur Mahotiere, Clarence Johnson, and Philamenia Howard

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James M. Garner, Gary J. Keever, D. Joseph Eakes, and J. Raymond Kessler

A study conducted in 1995 and repeated in 1996 determined the effects of repeated BA applications and subsequent repeated removals on yields of offsets in Hosta Tratt. (Funkia K. Spreng; Niobe Salisb.) stock plants. Two hosta cultivars, `Francee' and `Francis Williams', received zero, one, two, three, or four foliar applications of benzyladenine (BA) at 3000 mg·L-1. Plants receiving multiple applications were retreated at 30-day intervals following offset removal from all plants. A single BA application stimulated offset formation in both cultivars in both years, but repeated applications were necessary for a continued response following offset removal. Total offset yield increased linearly as the number of BA applications increased. At 120 days after the first treatment in 1995, `Francee' plants receiving four applications had produced an average of 22 offsets, and `Francis Williams' plants 18 offsets, whereas control plants produced 9.8 and 0 offsets, respectively. Similar data for 1996 were 31.2 offsets for `Francee' and 22.4 offsets for `Francis Williams,' whereas control plants produced 6.8 and 2.6 offsets, respectively. Offset stage of development, as indicated by leaf number, and growth index generally were not affected by BA treatment. No phytotoxicity was observed, and plant appearance was enhanced due to the outgrowth of BA-stimulated lateral buds. Chemical name used: N-(phenylmethyl)-1H-purin-6-amine (benzyladenine, BA).

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Joann M. McLaughlin and Duane W. Greene

Fruit-bearing limbs of five apple (Malus domestics Borkh.) cultivars varying in degree of biennial bearing were either unsprayed or received five repeat applications of BA at 50 mg·liter-1 with daminozide at 2000 mg·liter-1. BA and daminozide increased early appendage formation of potential flower buds, but this increase was sustained only on `Early McIntosh' and `Baldwin'. BA and daminozide increased return bloom on all treated limbs, with the exception of `Early McIntosh'. The annually bearing cultivars McIntosh and Delicious initiated 20 appendages before the formation of floral parts, while the biennially bearing cultivars Golden Delicious, Baldwin, and Early McIntosh initiated 19, 18, and 22 appendages, respectively. Flower removal before bloom increased appendage formation on the biennial cultivar Baldwin but not on the annual cultivar Delicious. Chemical names used: N -(phenylmethyl) -1 H-purine-6-arnine (BA); butanedioic acid mono(2,2-dimethyihydrazide) (daminozide).

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D.C. Elfving and R.A. Cline

Benzyladenine (BA) applied postbloom at 125 and 250 mg·liter-1 thinned `Empire' apple (Malus domestica Borkh.) trees below commercial crop levels but thinned less than thidiazuron (THI) at 62 and 125 mg·liter-1. Ethephon (ETH) applied up to 250 mg·liter-1 reduced fruit set only slightly. When BA was tank-mixed with ETH, thinning was the same as with BA alone. Although THI thinned more, BA resulted in a larger increase in fruit weight. Seed development was nearly eliminated by THI, but was unaffected by either BA or ETH. Thinner effects on foliar nutrient concentrations were associated with changes in fruit load but not shoot growth. The effects of BA and ETH on fruit-flesh nutrient concentrations were similar to their effects on foliar nutrient concentrations. Although THI thinned strongly and produced large changes in foliar nutrient concentrations and seed count, THI resulted in virtually no changes in fruit-flesh nutrient concentrations. Chemical names used: N-(phenylmethyl)-1 H-purine-6-amine (benzyladenine); 2-chlorophosphonic acid (ethephon); N-phenyl-N'-1,2,3-thiadiazol-5-ylurea (thidiazuron).

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Richard L. Harkess and Robert E. Lyons

BA and GA4+7, were applied to vegetative, mature Rudbeckia hirta plants at the beginning of long days (LD). There were no synergistic effects, but BA inconsistently affected branching and had no effect on flowering. Floral initiation of the terminal inflorescence was promoted by GA4+7, although axillary inflorescences were not. Increasing GA4+7 levels decreased the time to terminal inflorescence anthesis. However, the interval between the terminal and second axillary inflorescence anthesis was increased. The net result was no significant effect on the time to second axillary inflorescence anthesis. Gibberellins may enhance the LD effect on the apical meristem of Rudbeckia, but axillary meristems, which initiate later, remained unaffected. Chemical names used: benzyladenine (BA), gibberellin4+7, (GA4+7).

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Martin J. Bukovac, John C. Neilsen, and Jerome Hull Jr.

Generally, NAA is effective in inducing fruit thinning in `Delicious'. Although significant thinning may be induced, fruit size at harvest may not be closely related to crop load. Further, the magnitude of response to NAA may vary markedly between seasons. Herein, we present an analysis of response of `Redchief Delicious' over several years (tree age 11–14 years old) to high-volume sprays of NAA (15 mg·L–1), BA (25-50 mg·L–1), and CPPU (5 mg·L–1) at KFD of 8–12 mm. A single tree was used for each treatment replicated four to six times and response was measured by yield and fruit size distribution for each tree. In eight experiments over 4 years, NAA resulted in an average 22% reduction in yield, a 5.1% reduction in large fruit (70 mm+) and 2% reduction in small (<64 mm) fruit compared to NTC. There was a marked variation in response among years. Over 4 years, BA averaged a 5% decrease in yield, a 15% increase in large fruit and a 21% decrease in small fruit. In contrast, when NAA was combined with BA at 25-50 mg·L–1, yield decreased an average of 30%, large fruit decreased by 68%, and small fruit increased 8-fold (2.54 vs 20.6 kg/tree). CPPU alone (2-year study) had no significant effect on yield, but increased large fruit by 60% and significantly reduced production of small fruit. When CPPU was combined with NAA, yield was reduced in both years and the amount of large fruit was increased in 1995, but decreased in 1996. NAA had a very inhibitory effect on fruit size in 1996. One explanation may be that the crop was produced by lateral fruit (king flowers were lost to frost), and NAA has a greater inhibitory effect on lateral than king fruit. Results will be discussed in relation to studies with `Jonathan' and `Empire'.