through micropropagation ( Smagula and Lyrene, 1984 ). Previous information indicated that seedlings and micropropagated lowbush blueberries became established and spread faster than conventional rooted cuttings ( Debnath, 2007 ). Although plants grown
Suzanne M. Dethier Rogers and Sharon Banister
A micropropagation system was developed for micropropagation and rooting of Notholaena spp. Shoot cultures were initiated from mature sori on leaflets of the Notholaena cultivar Sun-Tuff and cultured on gelled Murashige and Skoog (MS) medium without hormones. Rooting and plant growth were evaluated on gelled MS, vermiculite moistened with liquid MS, or vermiculite moistened with water. Vermiculite wetted with MS was superior in promoting frond and root development. High humidity was not needed to acclimatize the plants ex vitro.
Georgia Vlachou, Maria Papafotiou, and Konstantinos F. Bertsouklis
). The aim of the present work was to develop efficient protocols for seed and clonal propagation of C. nepeta to facilitate its introduction in the horticultural and pharmaceutical industry. Seed germination, as well as micropropagation starting with
Boling Liu, Hongzhou Fang, Chaorong Meng, Ming Chen, Qingdong Chai, Kai Zhang, and Shijuan Liu
number of offshoots produced by the parent plants ( Mycock et al., 1997 ; Rogers, 1993a ). Consequently, micropropagation has been considered as an attractive way of propagating Haworthia genus plants. This method facilitates the propagation of plants
Byoung Kyong Jeong, Kazuhiro Fujiwara, and Toyoki Kozai
Autotrophic micropropagation has advantages over conventional micropropagation and can reduce costs of plantlet production. In this article, we describe advantages of autotrophic micropropagation and a practical and formulated method of enriching culture rooms with CO2.
Dao-Shun Huang, Jingkun Ho, and Ralph Backhaus
Tissue culture methods have been developed for a number of desert-adapted species which have potential economic value. These species include gum tragacanth (Astragalus gossypinus), candelilla (Euphorbia antisyphilitica), guayule (Parthenium argentatum), cliff rose (Cowania stansburiana and C. subintegra), bottle tree (Brachychiton populneum), red squill (Urginea maritima), Arizona agave (Agave arizonica), and spider lily (Pancratium littorale). Axillary shoot proliferation for the dicotyledenous species of this group is induced using concentrations of 2 mg/L BAP, whereas the rooting requirements vary considerably. Micropropagation of the bulb-producing species, is accomplished by aseptic culture of bulb scales in darkness, followed by shoot proliferation of the bulblets and rooting. Micropropagation of Agave requires adventitious shoot formation from a callus intermediate followed by direct caulogenesis from subculture shoots and subsequent rooting.
The potential benefit of these desert-adapted species and the usefulness of the micropropagation procedures will be discussed.
Margaret W. Kirika, Jane W. Kahia, Lucien N. Diby, Eliud M. Njagi, Colombe Dadjo, and Christophe Kouame
rainy season ( Sarasan et al., 2011 ). On the other hand, micropropagation offers a rapid means of producing large quantity of clonal planting stocks and propagation of some commercial crops and also tree species that are difficult to establish
Sadiye Hayta, Mark A. Smedley, Jinhong Li, Wendy A. Harwood, and Philip M. Gilmartin
may also be used to increase the population size of plants used as parents in F 1 seed production. Micropropagation techniques offer the fastest way of propagating these varieties. The first report of Primula micropropagation ( Coumans et al., 1979
Allison D. Oakes, Tyler Desmarais, William A. Powell, and Charles A. Maynard
influence of container dimensions in the multiplication rate of regenerating plant cell cultures, p. 275–277. In: Plant micropropagation in horticultural industries: Preparation, hardening and acclimatization Symposium. Belgian Plant Tissue Culture Group
Danny L. Barney, Omar A. Lopez, and Elizabeth King
micropropagation of other Vaccinium species, including highbush blueberry ( V. corymbosum ) ( Reed and Abdelnour-Esquivel, 1991 ), lowbush blueberry ( V. angustifolium ) ( Brissette et al., 1990 ; Debnath, 2004 ), cranberry ( V. macrocarpon ) ( Marcotrigiano and