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Chandra Thammina, Mingyang He, Litang Lu, Kaishuang Cao, Hao Yu, Yongqin Chen, Liangtao Tian, Junmei Chen, Richard McAvoy, Donna Ellis, Degang Zhao, Yuejin Wang, Xian Zhang, and Yi Li

, although regeneration from endosperm tissues is often technically challenging ( Thomas and Chaturvedi, 2008 ). In this article, we report for the first time in vitro regeneration of triploid plants from immature and mature endosperm explants of E. alatus

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Jin Cui, Juanxu Liu, Min Deng, Jianjun Chen, and Richard J. Henny

regeneration through either somatic embryos or protocorm-like bodies in which well-rooted plantlets could be produced for ex vitro transplanting. Regeneration through somatic embryos or protocorm-like bodies is also the desired system for genetic transformation

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Samir C. Debnath and Danny L. Barney

to introduce new traits into selected plants, to multiply clonal plants, and to develop suitable cultivars in a minimum time. The ability to regenerate plants is crucial to the successful application of in vitro methods ( Cao and Hammerschlag, 2000

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Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut

material for shoot multiplication of Paphiopedilum hybrids ( Huang et al., 2001 ). Nhut et al. (2007) studied the in vitro stem elongation of shoot-derived plantlets of P. delenatii to obtain stem nodes for effective shoot regeneration and

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Jane Kahia, Siaka Kone, Lucien Diby, Georges Ngoran, Colombe Dadjo, and Christophe Kouame

pattern which can occur ( Figueira and Janick, 1995 ). Plant regeneration through somatic embryogenesis provides an alternative approach for propagation of plants. Somatic embryogenesis depends on the ability of somatic plant cells to dedifferentiate and

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Mary C. Christey and Elizabeth D. Earle

Peduncle explants from 12 Brassica oleracea L. lines representing five varieties [broccoli (italica), cabbage (capitata), cauliflower (botrytis), Chinese broccoli (alboglabra), and rapid-cycling B. oleracea] readily regenerated shoots in vitro. Average regeneration rates of more than 75% were obtained for most lines, with up to 35 shoots per explant. Shoots were visible within 7 to 10 days. Initial regeneration was polarized, occurring mainly from the basal end of explants. Linsmaier-Skoog-based medium containing 1 mg BA/liter was suitable for shoot regeneration from all 12 lines tested. Plants were rooted on hormone-free medium and transferred to soil. Chemical name used: benzyladenine (BA).

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Marijana Jakše, Pablo Hirschegger, Borut Bohanec, and Michael J. Havey

mixoploid inflorescences and might result in normal flowers capable of self-pollination and production of homozygous DH seeds. Alan et al. (2007) induced somatic regeneration from flower buds of haploid plants and suggested that this method might be

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Wagner A. Vendrame, Ian Maguire, and Virginia S. Carvalho

-vitro shoot induction and multiplication in cultures of Doritaenopsis . Plant regeneration, acclimatization, survival, and establishment were also evaluated. Materials And Methods Plant material and explant sterilization. Plants of

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Paula P. Chee

A simple procedure for regeneration of cucumber plants (Cucumis sativus L. cv. Poinsett 76) from cotyledon and hypocotyl explants has been developed. Somatic embryogenesis was induced on Murashige and Skoog (MS) salts and vitamins medium supplemented with 2,4-D at 2.0 mg·liter-1 and kinetin at 0.5 mg·liter-1. Development of embryos was accomplished on MS medium with NAA at 1.0 mg·liter-1 and kinetin at 0.5 mg·liter-1. Eighty-five percent of the mature somatic embryos formed showed a typical bipolar structure. All developed into morphologically normal plantlets when transferred to MS medium containing no growth regulators. Chemical name used: 2,4-dichlorophenoxyacetic acid (2,4-D).

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Nancy A. Reichert, Nathan R. Oakley, and Brian S. Baldwin

An adventitious regeneration protocol developed for Hibiscus cannabinus L. (kenaf) was attempted on various ornamental hibiscus species. Hibiscus syriacus (Althea, Rose of Sharon) has been successfully regenerated using the kenaf protocol. Leaf tissue from two cultivars (`Double Pink' and `Diana'—a triploid) was placed on kenaf regeneration media. Adventitious shoots emerged from both cultivars within 8 to 10 weeks. Shoots were then excised and placed on a medium for rooting. Additional hibiscus species have been evaluated for regeneration ability. Previous studies with kenaf determined the adventitious regeneration protocol could induce mutations (somaclonal variation) in the regenerants. Variations in kenaf stem color and flower shape were noted. Since many ornamental hibiscus are asexually propagated, once a desired mutant is identified, it could be maintained and propagated without loss of the unique trait(s).