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Lawrence W. Zettler, Sarah B. Poulter, Kris I. McDonald, and Scott L. Stewart

mineral salts (MOM) were subsequently exposed to Miracle-Gro ex vitro on Sphagnum . Conversely, few (<25) seedlings cultured in vitro on the low-nutrient medium (standard oat medium) were added to Sphagnum without Miracle-Gro. Given the rarity of the

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Wei-Ting Tsai and Chien-Young Chu

Arditti, J. 1992 Fundamentals of orchid biology Wiley NY Daguin, F. Letouzé, R. 1987 Vitreous plants in vitro : Relationship with ammonium content of the nutrient medium Acta Hort. 212 259 261

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L. Zhang, J.R. Livingstone, Y. Tarui, and E. Hirasawa

element in the nutrient medium for the maintenance of hibiscus plants under low-illumination conditions. There have been reports on the use of CA to increase the vase life of cut flowers, but this is the first report on its use in intact plants

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Jiarou Liu, Bo Wu, Tao Xie, Aiping Luan, Yaqi Ding, Zhichun Zhang, Yehua He, and Zhike Zhang

. 2006 Influence of NaClO on nutrient medium sterilization and on pineapple ( Ananas comosus cv Smooth Cayenne) behavior Plant Cell Tissue Organ Cult. 86 3 375 378 10.1007/s11240-006-9121-3 Thomas, T

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Milica Ćalović, Chunxian Chen, Qibin Yu, Vladimir Orbović, Frederick G. Gmitter Jr, and Jude W. Grosser

treatment with this formulation was toxic ( Table 1 ). On treatment with enzyme mix A, protoplasts busted and their content spilled into the nutrient medium. Highest protoplast yield across all treatments was achieved with the use of enzyme mix C and cell

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Humberto Núñez-Moreno, James L. Walworth, Andrew P. Pond, and Michael W. Kilby

, 1987 ). It has also been noted that low leaf Zn is accompanied by high leaf Mn concentrations ( Kim et al., 2002 ). Additionally, increasing the Mn concentration in a nutrient medium from 0 to 14.78 g·L −1 decreased leaf Zn levels in ‘Desirable’ pecan

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Mary Lewis, Matthew Chappell, Donglin Zhang, and Rebekah Maynard

and Pearson, 1948 ; Whiting, 1943 ). Embryo abortion is difficult to overcome; however, by prematurely harvesting seeds, removing the embryo, and aseptically culturing the embryo on nutrient medium, pod abortion and developmental failure could be

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María del Carmen Vadillo-Pro, Luis Hernández-Sandoval, Guadalupe Malda-Barrera, María Luisa Osorio-Rosales, and Martín Mata-Rosas

Beaucarnea species ( Contreras et al., 2008 ). To initiate in vitro propagation, plant growth regulators (PGRs) can be applied to explants following two different methods: 1) traditionally the explants are placed on nutrient medium containing PGRs for 3

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Constantinos Tzerakis, Dimitrios Savvas, Nick Sigrimis, and Georgios Mavrogiannopoulos

assessment of Mn uptake in water culture experiments should be based on total Mn deposition in plant tissues and not on Mn removal from the external nutrient medium. Fig. 6. Apparent uptake (AA) of manganese (Mn) and zinc (Zn) from the nutrient solution vs

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He Li and Donglin Zhang

methods ( Bridgen, 1994 ; De Jeu, 2000 ; Sharma, 1995 ). By culturing immature embryos or seeds in the nutrient medium, some can overcome the incompatibility and avoid abortion. Successful case has been documented for another member of heath family