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Lawrence W. Zettler, Sarah B. Poulter, Kris I. McDonald, and Scott L. Stewart

mineral salts (MOM) were subsequently exposed to Miracle-Gro ex vitro on Sphagnum . Conversely, few (<25) seedlings cultured in vitro on the low-nutrient medium (standard oat medium) were added to Sphagnum without Miracle-Gro. Given the rarity of the

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Hilda E. Lee-Espinosa, Joaquín Murguía-González, Benjamín García-Rosas, Ana L. Córdova-Contreras, Antonio Laguna-Cerda, Javier O. Mijangos-Cortés, Luis F. Barahona-Pérez, Lourdes G. Iglesias-Andreu, and Nancy Santana-Buzzy

ascorbic acid. Shoot induction and proliferation. The basal nutrient medium recommended by Murashige and Skoog (MS) ( Murashige and Skoog, 1962 ) was used in all culture media supplemented with 100 mg·L −1 myoinositol, 150 mg·L −1 citric acid, 100

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Arnon Dag, Ran Erel, Alon Ben-Gal, Isaac Zipori, and Uri Yermiyahu

, physiological influences, environment (light and temperature), composition of the nutrient medium, age, and the ontogenetic phase of the mother plant ( Németh, 1986 ). In the current study, we explored the effect of nutritional status of the stock plant on

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Milica Ćalović, Chunxian Chen, Qibin Yu, Vladimir Orbović, Frederick G. Gmitter Jr, and Jude W. Grosser

treatment with this formulation was toxic ( Table 1 ). On treatment with enzyme mix A, protoplasts busted and their content spilled into the nutrient medium. Highest protoplast yield across all treatments was achieved with the use of enzyme mix C and cell

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María del Carmen Vadillo-Pro, Luis Hernández-Sandoval, Guadalupe Malda-Barrera, María Luisa Osorio-Rosales, and Martín Mata-Rosas

Beaucarnea species ( Contreras et al., 2008 ). To initiate in vitro propagation, plant growth regulators (PGRs) can be applied to explants following two different methods: 1) traditionally the explants are placed on nutrient medium containing PGRs for 3

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Constantinos Tzerakis, Dimitrios Savvas, Nick Sigrimis, and Georgios Mavrogiannopoulos

assessment of Mn uptake in water culture experiments should be based on total Mn deposition in plant tissues and not on Mn removal from the external nutrient medium. Fig. 6. Apparent uptake (AA) of manganese (Mn) and zinc (Zn) from the nutrient solution vs

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Humberto Núñez-Moreno, James L. Walworth, Andrew P. Pond, and Michael W. Kilby

, 1987 ). It has also been noted that low leaf Zn is accompanied by high leaf Mn concentrations ( Kim et al., 2002 ). Additionally, increasing the Mn concentration in a nutrient medium from 0 to 14.78 g·L −1 decreased leaf Zn levels in ‘Desirable’ pecan

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He Li and Donglin Zhang

methods ( Bridgen, 1994 ; De Jeu, 2000 ; Sharma, 1995 ). By culturing immature embryos or seeds in the nutrient medium, some can overcome the incompatibility and avoid abortion. Successful case has been documented for another member of heath family

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Mary Lewis, Matthew Chappell, Donglin Zhang, and Rebekah Maynard

and Pearson, 1948 ; Whiting, 1943 ). Embryo abortion is difficult to overcome; however, by prematurely harvesting seeds, removing the embryo, and aseptically culturing the embryo on nutrient medium, pod abortion and developmental failure could be

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Guirong Li, Ran Quan, Chaohui Yan, Xiaojin Hou, and Huiling Hu

plastic petri dishes (60 mm diameter) that contained 8 mL of medium. The nutrient medium was prepared by dissolving 15 g sucrose, 0.005 g boric acid, and 0.002 g CaCl 2 in 60 mL of double-distilled water, then adding 1 g agar powder and bringing the