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Rachel S. Leisso, Ines Hanrahan, James P. Mattheis, and David R. Rudell

., 2010 ). Symptoms similar to soggy breakdown (sharply demarcated regions of browned flesh) were exacerbated in high CO 2 storage environments alongside typical CO 2 injury symptoms (browned flesh tissue containing lens-shaped pits), confounding visual

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Cary Pirone, Jodie V. Johnson, J. Martin E. Quirke, Horacio A. Priestap, and David Lee

and –22 V for (+) and (–) ESI, respectively, whereas the tube lens was operated at 0 V for both ESI polarities. Collision-induced dissociation (CID) was conducted with a parent ion isolation of 3 u, CID energy of 37.5%, qCID of 0.25, and CID time of 30

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Michael T. Deaton and David W. Williams

Analysts (AOSA) protocol for bermudagrass was followed with regards to light ( AOSA, 1998 ). Germination counts were made daily for 21 d postimbibition. Seeds were counted as germinated and removed when the radicle was visible under a ×1.75 magnifying lens

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Brian A. Kahn

was unaffected by intersowing with hairy vetch ( Vicia villosa ), barrel medic ( Medicago truncatula ), or black lentil ( Lens culinaris ) ( Guldan et al., 1996 ). Hairy vetch intercropped with pepper and managed as a winter annual increased the yield

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Joseph N. Wolukau, Xiaohui Zhou, and JinFeng Chen

. Warkentin, T. Taran, B. Vandenberg, A. 2002 Genetics of resistance to anthracnose and identification of AFLP and RAPD markers linked to the resistance gene in PI 320937 germplasm of lentil ( Lens culinaris Medikus) Theor. Appl. Genet. 106 428 434 Vos, P

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Josh H. Freeman, Stephen M. Olson, and Eileen A. Kabelka

taken to the laboratory and allowed to open. Samples were analyzed after anther dehiscence was verified using a hand lens. Pollen was removed from the anthers and placed on a slide. Viability was determined using the diaminobenzidine (DAB) test for

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Xiaohua Du, Mengye Wang, Aneta Słomka, and Huichao Liu

acid at 60 °C for 6 to 8 min. Finally, the material was stained with carbol fuchsin, flattened with a cover slip, and observed under a Nikon Eclipse 80i microscope (Nikon Corporation) with a magnification of 10 (eye lens) × 100 (field lens). The images

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Ying Gao, Hao Liu, Ningguang Dong, and Dong Pei

each tissue, 30 oil immersion light micrographs (100× objective lens, 10× ocular lens) were taken at each stage of walnut pistillate flower bud differentiation. Using the image analysis software Image-Pro Plus6.0 (Media Cybernetics, Silver Spring, MD

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James C. Fulton, Francisco O. Holguin, Robert L. Steiner, and Mark E. Uchanski

to areas at the exact focal plane of the lens. This approach allowed for greater definition in the fluorescent signal. In addition, this provided a comparison between the fluorescent signal observed with the stereo fluorescent microscope and the

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Taku Shimizu, Kazuma Okada, Shigeki Moriya, Sadao Komori, and Kazuyuki Abe

lens (EZ-1442, Olympus) were fixed vertically over a gray background at 0.45 m. The zoom lens was fixed at a 75° view angle, and the camera optimum exposure and white balance settings were determined with a silk gray card (version 2; Ginichi, Tokyo