preparation, use of feeder layers, complex media formulations, and numerous subcultures. The aim of the present study was to establish a simple and efficient protocol to obtain transgenic tomato plants of cv. Micro-Tom, including an optimized ex vitro
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Abraham Cruz-Mendívil, Javier Rivera-López, Lourdes J. Germán-Báez, Melina López-Meyer, Sergio Hernández-Verdugo, José A. López-Valenzuela, Cuauhtémoc Reyes-Moreno, and Angel Valdez-Ortiz
Meijun Zhang, Duanduan Zhao, Zengqiang Ma, Xuedong Li, and Yulan Xiao
production. The losses during acclimatization accounted for 20% to 50% of the plantlets ex vitro. Photoautotrophic micropropagation (PA), using a sugar-free medium and leafy explant, in which plantlets use CO 2 in the air as the sole carbon source, has
Kelly M. Oates, Darren H. Touchell, and Thomas G. Ranney
.75 ± 0.03, solidified with 7.5 g·L −1 agar, and maintained under standard culture conditions for 1 week. Rooted microshoots were then transferred ex vitro into 72-cell trays and maintained under mist in a completely randomized design. After 4 weeks
Enio Tiago de Oliveira, Otto Jesu Crocomo, Tatiana Bistaco Farinha, and Luiz Antônio Gallo
, this article describes a complete micropropagation system involving disinfection, in vitro multiplication, rooting, and hardening followed by ex vitro acclimatization procedures used to attain that objective, thus producing thousands of Aloe vera
Aekaterini N. Martini, Maria Papafotiou, and Stavros N. Vemmos
rooting percentage in a previous study ( Martini and Papafotiou, 2009 ). For acclimatization, plantlets from adult plants or sprouts of burned plants with well-developed roots were transferred ex vitro to 500-mL plastic containers on a mixture of peat
De Yue, André Gosselin, and Yves Desjardins
Photosynthesis and growth of in vitro-cultured strawberry plantlets (Fragaria × ananassa Duch. cv Kent) were investigated during a 4-week in vitro culture in a rooting medium and a 4-week ex vitro period. The leaves formed in vitro on a medium containing sucrose developed a positive photosynthetic capacity. At transplanting to the ex vitro environment, their photosynthetic rate was 12.76 μmol CO2/m2 per second, which was as high as that of leaves generated and grown in the greenhouse. During the ex vitro period, photosynthetic rates of in vitro-generated leaves decreased and dark respiration rates increased. However, in vitro leaves were photosynthetically active throughout the 4 weeks ex vitro. In the first 2 weeks of the ex vitro period, in vitro-generated leaves had an important contribution to the overall plantlets' photosynthetic capacity.
Jonathan D. Mahoney and Mark H. Brand
and multiplied in vitro were rooted ex vitro. Microshoots were dipped in indole-3-butyric acid rooting hormone (Hormidin 1; OHP Inc., Mainland, PA) and rooted in clear plastic salad trays containing a mix of 1 peat: 1 vermiculite. A completely
Davis D. Harmon, Darren H. Touchell, Thomas G. Ranney, Kedong Da, and Wusheng Liu
10 µM IAA. Medium pH was adjusted to 5.8. After 4 weeks, shoots were transferred ex vitro and acclimatized in growing media (2 peat:1 vermiculite, v:v) in 50-cell trays and placed under intermittent mist (10-s duration at 10-min intervals
Richard J. Henny, James R. Holm, Jianjun Chen, and Michelle Scheiber
amount, 90 control plants were randomly selected for transfer to the greenhouse (three plants from each of 10 controls for each of the three harvest dates). Ex vitro transfer. Shoots were transferred into 288-celled trays containing Vergro Container Mix A
Georgia Vlachou, Maria Papafotiou, and Konstantinos F. Bertsouklis
micropropagation could lead to a high proliferation rate like in other Mediterranean native species ( Papafotiou and Martini, 2016 ; Papafotiou et al., 2013 ). Further, the use of seedlings for either in vitro or ex vitro propagation could enhance the higher