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Aisu Gu, Wenfang Liu, Chao Ma, Jin Cui, Richard J. Henny, and Jianjun Chen

,4-dichlorophenoxyacetic acid or NAA. Although anthurium micropropagation has been intensively studied, many reported methods appeared to be ineffective; some require a prolonged time for regeneration and others only produce a limited number of adventitious shoots

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Rongpei Yu, Ying Cheng, Yanfei Pu, Fan Li, and Shugang Lu

optimal media for original shoot induction, adventitious shoot proliferation and plantlet growth, and the optimal substrate type for plantlet acclimatization. This new protocol will be beneficial for horticultural and medicinal applications of S

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I-Ling Lai, Chih-Wan Lin, Tsai-Yu Chen, and Wei-Hsin Hu

vitro hybrid regeneration techniques should be investigated and the corresponding control mechanism determined before this hybrid is promoted for commercial use. Furthermore, light quality influences adventitious shoot regeneration ( Burritt and Leung

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María Victoria González, Manuel Rey, and Roberto Rodríguez

A simple and reliable protocol for plant regeneration from petioles of micropropagated plants of kiwifruit [Actinidia deliciosa (A. Chev) Liang and Ferguson, var. deliciosa `Hayward'] is described. Morphogenic callus was initiated by culturing petioles taken from in vitro-propagated plants. From the media tested, Cheng's K(h) medium plus 0.1 μm IAA, 4.5 μm zeatin, and 2% sucrose was the best for callus induction, maintenance, and shoot bud formation and development. Bases of developed shoots were immersed in 5 mm IBA for 15 seconds; subsequent culture in half-strength K(h) basal medium achieved 82% rooting. Regenerated plantlets were successfully transplanted to soil with 97% survival. Chemical names used: indole-3-acetic acid (IAA); indole-3-butyric acid (IBA); 2-methyl-4-(1H-purin-6-ylamino)-2-buten-1-ol (zeatin).

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Dongliang Qiu, Xiangying Wei, Shufang Fan, Dawei Jian, and Jianjun Chen

study was intended to develop protocols for regeneration of four blueberry cultivars whose protocols for adventitious shoot production have been not reported. As part of the effort, two of the four cultivars were successfully regenerated through indirect

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Boling Liu, Hongzhou Fang, Chaorong Meng, Ming Chen, Qingdong Chai, Kai Zhang, and Shijuan Liu

-naphthaleneacetic acid on the growth rate of callus of H. turgida . Data recorded after 30 d. Adventitious shoot differentiation. Each callus was cut into small pieces of around 2 cm 2 and transferred to the MS medium containing 0, 1.0, 2.0, or 3.0 mg·L −1 BA in

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Jin Cui, Juanxu Liu, Jianjun Chen, and Richard J. Henny

density of 50 μmol·m −2 ·s −1 . Table 3. Frequency of adventitious shoot formation from sprouted-seed explants derived callus piece (1 cm 3 ) and shoot number of Chlorophytum amaniense ‘Fire Flash’ after culture on a Murashige and Skoog basal medium z

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María del Carmen Vadillo-Pro, Luis Hernández-Sandoval, Guadalupe Malda-Barrera, María Luisa Osorio-Rosales, and Martín Mata-Rosas

( Hernández et al., 2009 ). Because of this situation, it is critical to take action to guarantee its continued survival and sustainable use. Fig. 1. ( A ) Adult individual of Beaucarnea purpusii on wild; ( B ) initial growth of adventitious shoots from

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Ze Li, Xiaofeng Tan, Zhiming Liu, Qing Lin, Lin Zhang, Jun Yuan, Yanling Zeng, and Lingli Wu

. oleifera that decreases the regeneration period. Adventitious shoot organogenesis is an easy and fast plant regeneration method that can be induced via direct or indirect pathways. Hypocotyl explants have been used to achieve regenerated plants in many

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Juanxu Liu, Min Deng, Richard J. Henny, Jianjun Chen, and Jiahua Xie

shoot induction. There were four callus pieces per petri dish and five dishes per treatment. Culture conditions. The culture of bud, leaf, and stem explants in callus and shoot induction as well as the rooting of adventitious shoots took place in