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adjusted to a pH of 6 to 7 and was partitioned against hexane three times. The pooled aqueous residue was then adjusted to pH 2.5 with HCl and partitioned against ethyl acetate. The pooled ethyl acetate phase was partitioned against a potassium phosphate

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each sample; PH2, SC2, RE, and FU). NPK was applied in all treatments, including the control. Table 1. Properties and composition of soils used in the study. Table 2. Biostimulant treatments. The coating of seeds was achieved as follows: seeds were

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)] in tap water (pH 2.8, EC 0.49 dS·m −1 ); 3) 10 g·L −1 a propriety mixture of sugar, acidifier and a biocide [FLO (Floralife ® Crystal Clear packets; Floralife, Walterboro, SC)] in tap water (pH 3.1, EC 0.50 dS·m −1 ); 4) deionized water (pH 3.8, EC

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pathway ( Forkmann, 1991 ; Holton and Cornish, 1995 ; Mol et al., 1998 ; Wiering and deVlaming, 1984 ). Additionally, genetic differences in flower color resulting from modifications in the pH were explained by genes Ph1, Ph2 , and Ph6 ( Griesbach

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were eluted using a mobile phase consisting 1% (v/v) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. Isocratic elution was performed with a flow rate of 0.75 mL·min −1 . Peaks were detected at 320 nm using a Waters 2487 dual

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. Grimsley, N. 1998 Genetic mapping of Ph-2 , a single locus controlling partial resistant to Phytophthora infestans in tomato Mol. Plant Microbe Interact. 11 259 269 10.1094/MPMI.1998.11.4.259 Morrison, L. Skaggs

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chromatography (HPLC) separation of tartaric, malic, and oxalic acids was achieved using a Prevail Organic Acid column 250 × 4.6 mm, 5-μm (Alltech Associates, Deerfield, IL); the mobile phase was 25 m m KH 2 PO 4 adjusted to pH 2 with phosphoric acid at a flow

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Waters 600E pump (Waters Corp., Milford, MA). Phenolic acids were eluted using a mobile phase consisting of 1% (by volume) formic acid in aqueous solution: acetonitrile: 2-propanol (70:22:8), pH 2.5. The column temperature was set to 30 °C and an

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.L. Methods of soil analysis. Part 2 2nd Ed Agron. Monogr. 9. ASA and SSSA Madison, WI Landschoot, P. 2007 Managing soil pH in turf Grounds Maintenance, Penton Media, Inc 21 Nov. 2007 < http://grounds-mag.com/mag/grounds_maintenance_managing_soil_ph

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interlinked columns Supelco LC-18 (250 × 4 mm); 30 °C; mobile phase: 1% phosphoric buffer (pH = 2.5), flow rate: 0.8 mL·min −1 . Anthocyanins were determined by pH differential method ( Wrolstadt, 1976 ) with Cary 300E spectrophotometer (Varian, Australia

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