Trials were conducted at Plymouth, NC in 2004 and 2005 to determine the effect of halosulfuron on the yield and grade of white-skinned and red-skinned Irish potatoes when used in combination with different infurrow insecticides. Factors for the factorial design used included potato variety, halosulfuron timing, halosulfuron rate, and insecticide. In-furrow insecticides included imidacloprid. aldicarb, and phorate at 30.3, 293, and 233 g ai/1000 m of row, respectively. Halosulfuron was applied at 26.3 or 52.6 g ai/ha preemergence (PRE), postemergence, over the top (POST), or postdirected (P-DIR) to the potatoes. Preemergence applications of halosulfuron were made after last hilling of the bed, POST applications were made at early flowering, and P-DIR sprays were applied at late flowering. Crop injury was evaluated visually at 2 and 4 weeks after treatment (WAT). Potatoes were dug and graded at maturity. Data was subjected to analysis of variance (ANOVA) with means separated using Fisher's protected LSD (p = 0.05). No interaction between insecticides and halosulfuron were observed. However, some yield differences were seen due to halosulfuron alone. Minimal (<10%) injury was seen with PRE and P-DIR applications. Substantial injury was only seen at 2 WAT, and only from POST treatments. At this timing, halosulfuron applied at the low rate injured potato 14-19% across the insecticides, while the high rate caused significantly higher injury (23% to 24%). Injury from the POST timing did affect yield. Higher levels of smaller potatoes (USDA Grade #1) were found in the POST treatments, when pooled over years, varieties and rates. This indicates that tuber development may have been delayed due to foliar injury. This is supported by the lower levels of USDA Grade #3 potatoes from POST applications compared to other timings. When pooled across years, varieties, and rates, the lowest total yields were with the POST timing.
R.B. Batts and A.W. MacRae
Rachel B. Elkins, Janet D. Turner, Steve Castagnoli, Clark F. Seavert, Elizabeth J. Mitcham, William V. Biasi, and Ann Colonna
Assessing consumer acceptance is an important aspect of cultivar evaluation. Since 2002, about 2700 consumers have participated in pear preference surveys. Surveys were conducted on multiple dates and at multiple venues from 2002 to 2005 in Oregon and northern California. Survey participants were asked to indicate their preference for pears based on size, appearance, taste, and overall preference. They were also asked to indicate what attributes they liked or disliked about their favorite and least favorite varieties and to indicate their level of purchase intent. Each survey consisted of four to six cultivars, including at least one standard commercial comparison; i.e., Bartlett, Bosc, or Anjou. Data was analyzed (RCBD; Friedman Analysis of Rank or ANOVA/Tukey's HSD) at the OSU Food Innovation Center Experiment Station using Compusense® five v.4.6 software (Guelph, Ont., Canada). Results indicated several alternative possibilities for both summer and winter sales. Among the most preferred cultivars (variable between states) were Anjou (commercial standard winter pear), Bartlett (commercial standard summer pear and most-consumed cultivar), Blake's Pride, Cinnamon, Concorde, and 71655-014. Other major findings were preference for large pears for adults and small for children, overall liking based on sweetness and flavor rather than skin color, and general lack of knowledge of many commercial pear cultivars. Sensory evaluation surveys will be continued in 2006 in California, with focus on differential harvest times for selected preferred cultivars. Consumer preference data is being combined with production and postharvest quality data in order to provide the pear industry a comprehensive data set on potential alternative cultivars.
Soon O. Park*, Kevin M. Crosby, Jonathan W. Sinclair, Kilsun Yoo, and Leonard M. Pike
Sucrose, fructose, total sugars and soluble solids are major factors in determining mature melon fruit sweetness. Bulked segregant analysis was utilized to detect RAPD markers associated with QTL for sucrose, total sugars and soluble solids in an F2 population from the ananas melon cross of Deltex (high sugars) × TGR1551 (low sugars). Sucrose, glucose, fructose and total sugar data were obtained from 108 F2 plants by means of HPLC. Clear separations for sucrose, total sugars and soluble solids between Deltex and TGR1551 were observed, whereas slight differences for glucose and fructose were found. Continuous distributions for sucrose, total sugars and soluble solids were observed in the F2 population indicating quantitative inheritance for the sweetness traits. A significant negative correlation was observed between sucrose and glucose (r = -25) or fructose (r = -0.31). A significant positive correlation was noted between sucrose and total sugars (r = 0.80) or soluble solids (r = 0.64). Three low and high DNA bulk pairs for sucrose, total sugars and soluble solids were developed. A total of 360 primers were used to simultaneously screen between the low and high bulks, and between Deltex and TGR1551. Sixty-eight RAPD markers were polymorphic for the low and high bulks. Of the 68 markers, 24 were found to be significantly associated with sucrose, total sugars or soluble solids on the basis of single-factor ANOVA. Marker OM15.550 was consistently associated with QTL affecting sucrose, glucose, fructose, total sugars and soluble solids, and accounted for 7% to 25% of the phenotypic variation for the traits. These markers associated with the sugar synthesis QTL could be useful to transfer these genes into a low sugar cultivar to enhance the fruit sweetness.
Kathryn S. Orvis and Irwin L. Goldman
Heart attack and stroke, a leading cause of death in the United States, have been associated with blood platelet aggregation. Onion extract inhibits blood platelet aggregation both in vitro and in vivo. Current trends toward natural foods and health remedies may point to the importance of onion-induced antiplatelet activity (OIAA). The genetic control of OIAA has yet to be revealed. One-hundred-eighty-three F3 families were derived from a long-day mild inbred line crossed to a long-day pungent inbred line that differ by for OIAA by 67%. Families were grown in a RCB design with two replications in muck soil (Randolph, Wis.) in 1997. Extracts were made from crushing bulb tissue in a mechanical juicer. F3 families were evaluated for OIAA and soluble solids (SS). OIAA was measured by electrical impedance aggregometry using two human blood donors. Endpoint (ohms) and slope of the aggregation curve were recorded. SS were measured by refractometry. F3 families were significantly different for OIAA and SS (P < 0.0001) in the ANOVA. A strong positive correlation of 0.96 was revealed for slope of curve and endpoint across families, replications, and blood donors. This correlation has not been previously reported for onion and suggests that for these families, descriptions of OIAA based on either rate of aggregation or endpoint are functionally equivalent. Both SS and OIAA exhibit transgressive segregation in this group of F3 families. Twenty percent exhibit OIAA stronger than the pungent parent and 5% were less than the mild parent. The family with the highest OIAA was 4-fold higher than the pungent parent of the cross, which could be useful in future onion breeding efforts. In addition, transgressive segregation in these families aids in QTL investigations for OIAA, SS and other economically important traits.
Brent L. Black and Richard H. Zimmerman
Highbush blueberry plants require low-pH, well-drained sandy soils. To increase the range of sites available for highbush blueberry production, by-products were tested as constituents in soilless media and as soil amendments. By-products, including coal ash, municipal biosolid compost, leaf compost, and acid peat, were combined in different proportions and compared to Berryland sand (alone) and Manor clay loam (alone and compost-amended) for a total of 10 media treatments. The pH of all treatment media was adjusted to 4.5 with sulfur. One-year-old tissue-cultured plants of `Bluecrop' and `Sierra' were planted in 15-L pots containing the pH-adjusted treatment media in 1997, producing their first substantial crop in 1999. For the 1999 crop, ripe fruit was harvested at weekly intervals over 5 weeks. ANOVA for yield indicated a significant cultivar × media interaction. `Bluecrop' appeared more sensitive to media treatment as yields on Manor clay loam were 80% less than on Berryland sand. Yields of `Bluecrop' on coal ash-compost mixes were similar to that of Berryland sand, and 1:1 coal ash:compost mixes produced significantly higher yields than did the 3:1 mixes. Yield of `Sierra' on Manor clay loam was 41% less than on Berryland sand, and plants growing on soilless mixes yielded 17% to 58% more than those on Berryland sand. `Bluecrop' fruit size was greatest for Berryland sand, but did not differ significantly among coal ash-compost mixes. For all media treatments, `Sierra' fruit size was inversely correlated with yield. Fruit from `Bluecrop' plants on coal ash-compost mixes ripened slightly earlier than on Berryland sand, but ripening date of `Sierra' did not vary significantly with soil treatment. The potential for employing these by-product mixes in small-scale commercial blueberry production will be discussed.
S. Serce and J.F. Hancock
A common complaint with day-neutral strawberries is that they perform poorly in mid-summer heat. Since most modern day-neutral cultivars are derived from the same Fragaria virginiana ssp. glauca clone from Utah, we felt it prudent to search for alternate sources of day-neutrality that were more heat-tolerant. We compared the sexual and vegetative performance of nine F. virginiana clones from a wide range of environments including the Utah site, and four F. × ananassa day-neutral types (`Aromas', `Fort Laramie', `Ogallala', and `Tribute') under constant temperatures of 18, 22, 26, and 30 °C and 12-h days. `Aromas' and `Tribute' carry the Utah source of day-neutrality, while `Fort Laramie' and `Ogallala' are old cultivars that have a different, complex background. After a 4-week period of acclimation, we counted the number of crowns, inflorescences, flowers, stolons, and daughter plants that emerged over a 10-week period, and measured the dry weights of component parts. ANOVA tables revealed that temperature regime (T), genotypes (G), and T*G were significant for flower number (FLN) and total dry matter accumulation, while species and T*G were significant for daughter plant number (DPN). Mean FLNs across the four temperatures were 6.8, 3.7, 3.3, and 1.2, while mean DPNs were 0.7, 0.9, 0.7, and 1.8. F. virginiana clones averaged 3.8 FLNs and 1.8 DPNs, while the F. × ananassa clones averaged 4.1 FLNs and 0.2 DPNs. There was generally more variability among the F. virginiana clones than the F. × ananassa clones, but the F. × ananassa cultivars, `Fort Laramie' and `Ogallala', performed best at 30 °C. The Wasatch clone did not flower in any treatment, suggesting it is not day-neutral.
Greg McCollum and Kim D. Bowman
The objective of this experiment was to compare fruit-quality parameters of ‘Ray Ruby’ grapefruit grown on seven rootstocks. Four recent releases from the United States Department of Agriculture (USDA) rootstock breeding program, ‘US-852’, ‘US-897’, ‘US-942’, and ‘US-812’ (all Citrus reticulata × Poncirus trifoliata hybrids), ‘x639’ (C. reticulata × P. trifoliata), along with industry-standard ‘Sour Orange’ and ‘Swingle’ citrumelo were evaluated in a commercial orchard trial in Indian River County, FL. Fruit-quality data were collected in 2011–12 (eight harvests), 2012–13 (five harvests), and 2014 (single harvest). In each season, rootstock effects on fruit size, total solids, and solids acid ratio were significant. ‘Sour orange’ and ‘Swingle’ produced the largest fruit, whereas ‘US-897’ (a semidwarfing rootstock) produced the smallest fruit. Peel thickness (measured only in the 2011–12 season) was greatest in ‘Sour Orange’ early in the season, but not toward the end of the season. Misshapen (“sheep-nosed”) fruit occurred more frequently on ‘Sour Orange’ than on other rootstocks, although the incidence of sheep-nosing was minor. Analysis of variance (ANOVA) for fruit-quality data collected in January of each of the 3 years confirmed that ‘Sour Orange’ and ‘Swingle’ produced the largest fruit and ‘US-897’ produced the smallest fruit. Total solids were the highest in ‘US-897’ and the lowest in ‘x639’ and ‘US-852’. Taken together, our data indicate that ‘US-942’ and ‘US-897’ rootstocks produced fruit with quality characteristics that equaled or exceeded ‘Sour Orange’ and ‘Swingle’, the two most common rootstocks used in the Indian River district.
Mathura Thillainathan and George C.J. Fernandez
A user-friendly SAS statistical and graphical application to classify genotypes evaluated under multiple sites is presented. First, the test sites are classified into three environments, LOW [(
electrolyte leakage (REL), and root dry weight (RDW) were log transformed. Mean separation tests were performed on means of transformed data; however, the results were presented using untransformed means. The corrected Table 1 (ANOVA) is as follows
Albert N. Kishaba, Steven J. Castle, Donald L. Coudriet, James D. McCreight, and G. Weston Bohn
Abbreviations: ANOVA, analysis of variance; AR HBJ, AR Hale's Best Jumbo; AR TM, AR Topmark; AR 45, aphid-resistant `PMR 45'; CMV, cucumber mosaic virus; HBJ, `Hale's Best Jumbo'; EN 50 , 50% infection; WMV, watermelon mosaic virus; ZYMV, zucchini