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Yuan Huang, Xue-qin Wang, Chun-yan Yang, and Chun-lin Long

understood. Here, 11 polymorphic microsatellite loci of P. amethystina were developed as potential tools to investigate the genetic structures of these species. Genomic DNA was extracted from leaf tissues using the cetyltrimethyl ammonium bromide (CTAB

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Deborah Dean, Phillip A. Wadl, Denita Hadziabdic, William E. Klingeman, Bonnie H. Ownley, Timothy A. Rinehart, Adam J. Dattilo, Brian Scheffler, and Robert N. Trigiano

actions be required ( Hamrick and Godt, 1996 ; Holsinger and Gottlieb, 1991 ; Newton et al., 1999 ). Microsatellites, also referred to as SSR (SSRs), are a class of codominant markers that are useful in assessing genetic diversity of plants due to their

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Deborah Dean, Phillip A. Wadl, Xinwang Wang, William E. Klingeman, Bonnie H. Ownley, Timothy A. Rinehart, Brian E. Scheffler, and Robert N. Trigiano

et al., 2008, 2009 ; Wang et al., 2010 ). Microsatellites are ubiquitous throughout genomes and codominant markers, often used in both interspecific and intraspecific diversity studies ( Gupta and Varshney, 2000 ). These genetic markers exhibit

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Sheng-Xi Liao, Xian-Jie Mi, Ai-Zhong Liu, Kun Li, Zhen-Yin Yang, and Bo Tian

diversity for this species. Unfortunately, very few molecular markers are available to investigate the genetic diversity for Chinese Incense-cedar to date. In this report, we describe development of 13 polymorphic microsatellite loci for ongoing population

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Valentina Scariot, Aziz Akkak, and Roberto Botta

(Wageningen, The Netherlands) for providing unpublished microsatellite loci primer sequences, Ben Vosman for the critical review of the manuscript, and Elena Costa for the help in morphological identification of rose accessions. We very much appreciated the

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Samuel G. Obae, Mark H. Brand, Bryan A. Connolly, Rochelle R. Beasley, and Stacey L. Lance

have limited molecular variation due to apomictic seed production ( Brand, 2009 ; Persson Hovmalm et al., 2004 ). The success of molecular plant breeding hinges on availability of genome-specific molecular tools such as microsatellites or simple

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Zahra Noormohammadi, Mehdi Hosseini-Mazinani, Isabel Trujillo, Luis Rallo, Angjelina Belaj, and Majid Sadeghizadeh

result of their multiallelic hypervariability, codominant nature, high information content, and amenability to automation, microsatellites are becoming one of the most popular and reliable marker systems for genetic characterization of varieties, genetic

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Aurora Díaz, Antonio Martín, Pilar Rallo, and Raúl De la Rosa

(SSRs), or microsatellites, are increasingly becoming the markers of choice for paternity analysis in plants, because they now offer an easy and reliable way to test the paternity of seeds and seedlings by checking the presence of parental alleles in the

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Shinichi Masuzaki, Naoyuki Araki, Naoki Yamauchi, Naoko Yamane, Tadayuki Wako, Akio Kojima, and Masayoshi Shigyo

Bulb onion (Allium cepa L.) has a very large genome composed of a high proportion of repetitive DNAs. Genetic analyses of repetitive sequences may reveal microsatellites in order to increase the number of genetic markers in onion. Thirty microsatellites were previously isolated from an onion genomic library (Fischer and Bachmann, 2000). A complete set of Japanese bunching onion (A. fistulosum) – shallot (A. cepa Aggregatum group) monosomic addition lines were used to assign these microsatellites to the chromosomes of A. cepa. Simplified PCR conditions for each microsatellite were determined and 28 of the 30 primer pairs amplified DNA fragments, of which 21 microsatellite markers were assigned to chromosomes of A. cepa. Subsequent mapping of these microsatellites will enable us to establish the chromosomal distribution of these markers.

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Jana Murovec, Natasa Stajner, Jernej Jakse, and Branka Javornik

et al., 2003 ; Yahata et al., 2005a , b ), but microsatellite markers are used much more nowadays because of their codominant nature and unambiguous results. Analysis of the genetic origin of haploid/diploid regenerants using microsatellite markers