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Andrew Mullin, Bárbara Nogueira Souza Costa, Jason Downing, and Amir Ali Khoddamzadeh

with this frequent watering schedule. This study aimed to establish a fast and efficient protocol for in vitro seed germination using different nutrient media and PGR combinations, and to optimize seedling acclimatization protocols using different

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Rashid Sultan Al-Obeed, Salah El-Hendawy, Mayada Kadri Seliem, and Khadija Rabeh Al-Harbi

shoot tips and nodes of D. ombet seedlings onto MS medium without plant growth regulators (PGRs) for 6 months produced sufficient stock of shoots for further experiments. Fig. 1. In vitro seed germination and shoot proliferation of Dracaena ombet

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Yujie Yang, Donglin Zhang, Zhihui Li, Xiaoling Jin, and Jinying Dong

many crops to overcome seed dormancy and shorten seed germination time ( Sharma et al., 1996 ). In vitro germination of immature embryos might be helpful to shorten breeding cycles and accelerate the breeding process. In vitro cultures, plant embryos

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Natalia R. Dolce, Ricardo D. Medina, Luis A. Mroginski, and Hebe Y. Rey

. 1A ). The plants used in this study came from the acclimatization of in vitro plants obtained by asymbiotic germination of seeds from different fruits acquired in local nursery gardens. The plants (2–3 years old) were nourished every 2 weeks with 0

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Georgia Vlachou, Maria Papafotiou, and Konstantinos F. Bertsouklis

of storage and temperature on C. nepeta seed germination ability, and 2) the effect of various plant growth regulators and agar concentrations on in vitro shoot proliferation, elimination of hyperhydricity problems, and microshoot rooting. Materials

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Wei-Ting Tsai and Chien-Young Chu

seeds germinated on the walls of the vessel where nutrient liquid remained and seeds germinated faster than those on a solid medium. Given that in vitro liquid culture has been used successfully to cultivate a number of important nonorchids such as

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Li Xu, Fangyun Cheng, and Yuan Zhong

embryos through in vitro immature embryo culture with seeds carrying young embryos as explants. Then, the effects of the inoculation methods and development stage were evaluated. The second step was to establish the seedlings via embryo germination, shoot

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Qi Zhang, Kevin Rue, and Sheng Wang

. Studies found a substantial reduction in buffalograss seed germination and seedling growth at 50 mM NaCl ( Reid et al., 1993 ; Wu and Lin, 1993 , 1994 ). Marcum (1999) and Marcum and Kopec (1997) assessed relative salt tolerance of eight species in

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Jason D. Lattier and Ryan N. Contreras

. Seedlings were germinated in a glasshouse under the conditions described previously. Data collected over winter included number of germinated seed, albino seedlings, and viable green seedlings. In vitro germination. Capsules progressed from pollination to

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Yan Yao, Yao Kong, Ping Zhang, Hua Zhang, Hong-di Huang, and Guang-guang Li

accelerate flowering, the technique of young embryo culture in vitro has also been used to shorten the lifecycles by circumventing the requirements of seed maturation and postharvest dormancy ( Mobini and Warkentin, 2016 ; Zheng et al., 2013 ). The suitable