Search Results

You are looking at 31 - 40 of 188 items for :

  • green fluorescent protein x
  • Refine by Access: All x
Clear All
Free access

Guo-qing Song, Hideo Honda, and Ken-ichi Yamaguchi

constitutive promoters when transgenes, including most of those conferring resistances to herbicides, insects, and diseases, are targeted principally in green tissues of sweetpotato. This is because leaf-specific instead of constitutive expression of target

Open access

Lie Li, Yu-xin Tong, Jun-ling Lu, Yang-mei Li, and Qi-chang Yang

artificial light is the sole light source for plant growth. In past decades, traditional artificial light sources, such as fluorescent, high-pressure sodium, and metal halide lamps, have been used in tissue culture, growth chambers, and greenhouses to

Free access

Jingkang Hu, Yingmei Gao, Tingting Zhao, Jingfu Li, Meini Yao, and Xiangyang Xu

., Shanghai, China) system in a 20-μL reaction system. The fluorescent dye 2 × Power SYBR Green PCR Master Mix (Vazyme, Nanjing, China) was used, and the reaction protocol was as follows: 95 °C for 6 min; followed by 40 cycles of 95 °C for 10 s, 58 °C for 20 s

Free access

Gordon J. Lightbourn, John R. Stommel, and Robert J. Griesbach

). Anthocyanin structural gene transcription requires the expression of at least one of each of three distinct transcription factor families: MYC, MYB, and WD40 ( Griesbach, 2005 ). The Myc gene family encode proteins characteristic of human MYC oncoprotein

Free access

Seenivasan Natarajan and Jeff S. Kuehny

heat-tolerant lines so that breeding programs can use these plants to improve the thermotolerance of new releases ( Prasad et al., 1999 ). Many organisms, including plants, accumulate heat shock proteins (HSP) in response to high nonlethal temperatures

Free access

Wei Hao, Rajeev Arora, Anand K. Yadav, and Nirmal Joshee

in the plants, including modifications in membrane lipid composition; increases in soluble sugars, amino acids, and organic acids; synthesis and accumulation of antioxidants and protective proteins; changes in hormone levels; and alterations in gene

Free access

Jin Jiao, Xing Liu, Juyou Wu, Guohua Xu, and Shaoling Zhang

-3′ and reverse primer, 5′-ATGAAATCATTGGAGCTGAAAGAGAT-3′. The PCR product cut by the SpeI and BamHI enzymes (TaKaRa) was ligated into PUC19 digested with SpeI and BamHI to generate the CaMV35S:PbrMAPK13-GFP (green fluorescent protein) construct

Free access

Shutian Tao, Danyang Wang, Cong Jin, Wei Sun, Xing Liu, Shaoling Zhang, Fuyong Gao, and Shahrokh Khanizadeh

containing the green fluorescent protein (GFP) reporter gene to generate a pCAMBIA 1302- PbrC4H -GFP fusion under the control of the CaMV 35S promoter. The pCAMBIA 1302 vector was used as the control. Subsequently, the control vector and the recombinant

Free access

Cai-Hong Jia, Ju-Hua Liu, Zhi-Qiang Jin, Qiu-Ju Deng, Jian-Bin Zhang, and Bi-Yu Xu

real-time RT-PCR analysis using Stratagene Mx3000P (Stratagene, CA) and SYBR Green as a fluorescent dye. The reaction mixture consisted of a 25-μL solution containing 12.5 μL 2 × SYBR Green PCR Master Mix, 0.5 μL ROX reference dye, and 100 ng reverse

Open access

Lili Dong, Tongrui Liu, Di Gao, Jing Li, and Jie Qian

of PhSDG8. A pCAMBIA1300 vector containing PhSDG8 fused to Green Fluorescent Protein (GFP) at its N terminus was constructed in this research. We used pCAMBIA1300 as a control. After transient transformation into N. benthamiana , GFP drived by 35S