Search Results

-distance transport of RNAs in grafted plants ( Table 1 ), indicating that some RNA molecules are systemic signals capable of regulating target genes/traits; and 3) plant virus vector systems that have been widely used for RNA production and delivery in woody plants

. Virus-induced silencing of PDS gene in the seedlings of rose. The 162-bp fragment of the PDS gene in rose was inserted into an ALSV-RNA2-based vector ( Fig. 2 ). The resulting virus was designated as pEALSR2L5R5-mulPDS (mulPDS-ALSV), and total RNAs

Center for Biotechnology Information GenBank for each Papaya ringspot virus isolate coat protein gene sequence is shown as well. RNA isolation, reverse transcription polymerase chain reaction, and degenerate primer design. Tissue weighing 100 mg was

, 2017 ). Plant diseases incited by viruses are a major limiting factor in commercial watermelon production worldwide. More than 10 viruses are known to be a problem in watermelon field production ( Ali et al., 2012 ; Wang et al., 2017 ). The major

Involvement of RNA2 for systemic infection of Cucumber mosaic virus isolated from lily on zucchini squash Virus Res. 97 1 6 Damicone, J.P. Edelson, J.V. Sherwood, J.L. Meyers, L.D. Motes, J.E. 2007 Effects of border crops and intercrops on control of cucurbit

et al., 2011 ), and Pepino mosaic virus ( Moerkens et al., 2015 ). ACLSV, Apple stem grooving virus (ASGV), and ASPV are widely distributed and commonly occurring in apples. All three are single-stranded RNA viruses, typically latent (symptomless

. Ultrathin sections stained with uranyl acetate followed by lead citrate were examined with a JEOL 1200 EXII transmission electron microscope (JEOL Ltd.). Molecular cloning and sequence analyses. Total RNAs were extracted from healthy and virus

other tested viral RNA, and none tested positive by ELISA for other viruses. This suggests that the experiment was valid and the disease ratings for PepYLCThV were not confounded with symptoms of other viruses. The Kruskal-Wallis test by ranks (α = 0

2017, with a current farm value of $579 million ( USDA, 2017 ). Worldwide, more than 10 viruses are known to be limiting factors for commercial watermelon production ( Romay et al., 2014 ; Wang et al., 2017 ). The major viruses affecting watermelon in

States are Florida, California, Texas, Georgia, and North Carolina ( USDA, 2017 ). Production increased from 1.2 million Mg in 1987 to 5.1 million Mg in 2017, with a farm value of $579 million ( USDA, 2017 ). Plant diseases caused by viruses are a major