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Cecilia McGregor, Vickie Waters, Savithri Nambeesan, Dan MacLean, Byron L. Candole, and Patrick Conner

, whereas genotype diversity was calculated as described by Tommasini et al. (2003) . The DNA used for SSR analysis was also used for amplification of the D04 sequence characterized amplified region (SCAR) marker reported to be linked to the Phyto 5

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Amnon Levi and Kai-shu Ling

2) and a sequenced characterized amplified region (SCAR) marker (designated as ZYRP) associated with ZYMV-FL resistance ( Harris et al., 2009 ; Ling et al., 2009 ). Fig. 1. Pedigree of USVL-380 showing the route of incorporating the Zucchini yellow

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Yuanfu Ji, Jay W. Scott, David J. Schuster, and Douglas P. Maxwell

, linkage analysis, and QTL mapping. All markers used in this study were PCR-based, including sequence-characterized amplified region (SCAR) and cleaved amplified polymorphic sequence (CAPS) markers taken from the public domain or designed from public

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Amnon Levi, Karen R. Harris-Shultz, and Kai-shu Ling

-assisted selection using two cleaved amplified polymorphic sequence (CAPS) markers in the eIF4E gene locus (CAPS1, CAPS2) as described by Ling et al. (2009) ( Fig. 2 ) and a sequenced characterized amplified region marker (designated as ZYRP) associated with ZYMV

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Yuanfu Ji, John W. Scott, and David J. Schuster

trays as described previously ( Fulton et al., 1995 ). All markers used in this study are PCR-based, including sequence-characterized amplified region (SCAR) markers and cleaved amplified polymorphic sequence (CAPS) markers taken from either the public

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Caihong Wang, Yike Tian, Emily J. Buck, Susan E. Gardiner, Hongyi Dai, and Yanli Jia

separated on 1.3% agarose gels, stained with ethidium bromide (0.5 μg·mL −1 ), and photographed under ultraviolet light. Conversion of random amplified polymorphic DNA markers into sequence-characterized amplified region markers. Polymorphic RAPD marker

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Gen-Fa Zhu and Dong-Mei Li

investigated Dendrobium species ( Wang et al., 2009 ). Transferring of these specific AFLP markers into sequence-characterized amplified region markers would be very useful for molecular diagnosis of Dendrobium species and hybrid cultivars. In conclusion

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Guojing Li, Yonghua Liu, Jeffrey D. Ehlers, Zhujun Zhu, Xiaohua Wu, Baogeng Wang, and Zhongfu Lu

). However, it is difficult to employ the AFLP technique directly in a MAS program or for map-based gene cloning because of its high cost and complicated methodology. Converting AFLP markers into “easy-to-use” markers, such as sequence-characterized amplified

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Jack E. Staub and Isabelle Y. Delannay

, 30 of 288 individuals were selected (selection intensity = 11%) as possessing the highest heterozygosity based on molecular marker profiles [19 mapped, simple sequence repeat (SSR) and sequence characterized amplified region (SCAR) marker loci

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Dario J. Chavez and José X. Chaparro

the probability of some false-positives/-negatives. Conversion of RAPD markers to sequence characterized amplified region-type markers is being pursued. The identification of RAPD markers associated with seedlessness from C. kinokuni constitutes an