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Masafumi Johkan, Genjirou Mori, Kazuhiko Mitsukuri, Keiichirou Mishiba, Toshinobu Morikawa, and Masayuki Oda

effective for multiplication of clones, it is expensive and often generates somatic mutations ( Padmanabhan et al., 1974 ). The complete decapitation method (CDM), which regenerates multiple shoots from the cut surfaces of the main and lateral stems of

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Seong Min Woo and Hazel Y. Wetzstein

as blueberry, cranberry, and rhododendron. This has led to the development of efficient plant regeneration protocols achieved through organogenesis from cultures derived from leaf tissue, shoot tips, and axillary buds. Plants regenerated from this

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Mohammed Elsayed El-Mahrouk, Mossad K. Maamoun, Antar Nasr EL-Banna, Soliman A. Omran, Yaser Hassan Dewir, and Salah El-Hendawy

per ovule, the number of embryo-like structures per callus, and the gynogenic percentage were recorded. The regenerated embryos and embryo-like structures were transplanted into MS medium without PGRs for their subsequent growth. The cultures were kept

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Carrie A. Radcliffe, James M. Affolter, and Hazel Y. Wetzstein

( Guerrant et al., 2004 ). A number of threatened and endangered woody species have been regenerated using tissue culture. Betula uber , confined to a single population, was propagated by axillary shoot proliferation ( Vijayakumar et al., 1990 ). Shoot tip

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James A. Stamp, Sheila M. Colby, and Carole P. Meredith

Adventitious shoots developed within 3 weeks from the petiolar stub and, less often, from wounded lamina tissues when leaves excised from nodal cultures of Vitis vinifera L. cvs. French Colombard and Thompson Seedless were cultured on solid Nitsch and Nitsch medium containing BAP at 2 mg·liter-1. The youngest leaf that could be excised, from 1 to 8 mm long, was the most responsive (90% of explants producing shoots compared to 16% for leaf 6). Removal of the lamina from the petiolar stub within the first 3 weeks of culture reduced shoot production. Increase in nodal culture age, without transfer to fresh medium, had no effect on subsequent regeneration from the youngest leaves but did reduce the regeneration frequency of leaves at the next position from 43% to 20%. In regularly subculture nodal cultures, the number of transfers had no effect on subsequent regeneration. Leaves from recently established shoot tip cultures were more responsive than leaves from nodal cultures. The frequency of shoot production was higher in laterally bisected than intact leaves (70% vs. 43%) due to additional regeneration from the distal leaf half at the sites of severed veins. Shoot outgrowth was promoted by the isolation and subculture of regenerating tissue to fresh regeneration medium. Petiolar stub removal promoted de novo shoot organogenesis from the resulting lamina wound. Shoots rooted at a high frequency on Murashige and Skoog medium with 1 mg IA-A/liter and produced morphologically normal plants. Chemical names used: 6-benzylaminopurine (BAP); indole-3-acetic acid (IAA).

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Eucario Mancilla-Álvarez, Marco A. Ramírez-Mosqueda, Samantha Arano-Avalos, Rosalía Núñez-Pastrana, and Jericó J. Bello-Bello

malanga, there are long-term in vitro conservation studies ( Bessembinder et al., 1993 ; Sant et al., 2008 ). However, there is no a study of in vitro preservation in the medium term to ensure regeneration of preserved material. However, establishing the

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Xiaoling Jin, Xijun Hu, Youping Sun, Donglin Zhang, and Ping He

genetic diversity. Micropropagation offers a rapid means of clonal regeneration for reforestation, gardening, and germplasm conservation ( Fenning and Gershenzin, 2002 ). Gao et al. (1996) reported that embryos, cotyledons, hypocotyls, terminal buds, and

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Jane Kahia, Peter Kanze Sallah, Lucien Diby, Christophe Kouame, Margaret Kirika, Simeon Niyitegeka, and Theodore Asiimwe

Correia et al. (2011) . However, there are no documented studies on the direct organogenesis in Tamarillo. An important advantage of direct organogenesis is the potential for maintaining genomic stability of regenerated plants, whereas regeneration via an

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Akira Sugiura, Yoshiko Matsuda-Habu, Mei Gao, Tomoya Esumi, and Ryutaro Tao

; Tao and Sugiura, 1992b ; Yokoyama and Takeuchi, 1976 ). Shoot tip culture of current-year shoots is possible ( Sugiura et al., 1986 ) as is plant regeneration from calli and cell cultures derived from dormant buds ( Tao et al., 1988 ; Tao and Sugiura

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Saad B. Javed, Abdulrahman A. Alatar, Mohammad Anis, and Mohamed A. El-Sheikh

the TDZ response. Our study was undertaken to analyze the response of different explants excised from the top, middle, and bottom of coral tree seedlings. Explants were exposed to different concentrations of TDZ to establish an efficient regeneration