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Tracy S. Hawkins, Nathan M. Schiff, Emile S. Gardiner, Theodor Leininger, Margaret S. Devall, Dan Wilson, Paul Hamel, Deborah D. McCown, and Kristina Connor

relative to some woody plants with episodic growth, such as species of Fagus and Juglans ( McCown and McCown, 1987 ). The initial ex vitro rooting treatment using a 10% auxin solution yielded somewhat low mean (± se ) rooting percentages of 30 ± 5

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How-Chiun Wu and Chun-Chih Lin

inconsistent. Furthermore, attempts to promote vegetative and root growth during ex vitro acclimatization have achieved limited success. Thillerot et al. (2006) reported very slow growth of P. cynaroides explants with no root growth after 2 months in ex

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Martín Mata-Rosas and Víctor M. Salazar-Rojas

shoot formation. ( A ) Flowering plant. ( B ) Multiple shoot formation from protocorms in Murashige and Skoog medium + 13.3 μM N 6 -benzyladenine. ( C ) In vitro-rooted shoots ready for ex vitro culture. ( D ) Plants obtained from in vitro culture after

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Nguyen Phuc Huy, Vu Quoc Luan, Le Kim Cuong, Nguyen Ba Nam, Hoang Thanh Tung, Vu Thi Hien, Dung Tien Le, Kee Yoeup Paek, and Duong Tan Nhut

Paphiopedilum micropropagation from ex vitro–derived explants has been relatively limited. Its difficulty has been caused by contamination of ex vitro–derived explants and the poor development of explants ( Huang, 1988 ; Stewart and Button, 1975 ). There have

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Martín Mata-Rosas, Rosario Julieta Baltazar-García, and Victor Manuel Chávez-Avila

BA 1 mg·L −1 and NAA 0.1 mg·L −1 ; ( E ) in vitro rooted shoots ready for ex vitro culture; ( F ) plantlets of O. tigrinum after successful establishment in soil for 2 months. Bar = 1 cm. PLBs = protocorm-like bodies; NAA = α-naphthaleneacetic acid

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Boling Liu, Hongzhou Fang, Chaorong Meng, Ming Chen, Qingdong Chai, Kai Zhang, and Shijuan Liu

Soil, Deli, Fuzhou, China; 1:1, v/v), and established in the greenhouse for simultaneous ex vitro rooting. The rooting percentage was calculated as follows: Table 4. Effect of 1-naphthaleneacetic acid on root induction in micropropagated plantlets of H

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Abraham Cruz-Mendívil, Javier Rivera-López, Lourdes J. Germán-Báez, Melina López-Meyer, Sergio Hernández-Verdugo, José A. López-Valenzuela, Cuauhtémoc Reyes-Moreno, and Angel Valdez-Ortiz

preparation, use of feeder layers, complex media formulations, and numerous subcultures. The aim of the present study was to establish a simple and efficient protocol to obtain transgenic tomato plants of cv. Micro-Tom, including an optimized ex vitro

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Meijun Zhang, Duanduan Zhao, Zengqiang Ma, Xuedong Li, and Yulan Xiao

production. The losses during acclimatization accounted for 20% to 50% of the plantlets ex vitro. Photoautotrophic micropropagation (PA), using a sugar-free medium and leafy explant, in which plantlets use CO 2 in the air as the sole carbon source, has

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Kelly M. Oates, Darren H. Touchell, and Thomas G. Ranney

.75 ± 0.03, solidified with 7.5 g·L −1 agar, and maintained under standard culture conditions for 1 week. Rooted microshoots were then transferred ex vitro into 72-cell trays and maintained under mist in a completely randomized design. After 4 weeks

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Enio Tiago de Oliveira, Otto Jesu Crocomo, Tatiana Bistaco Farinha, and Luiz Antônio Gallo

, this article describes a complete micropropagation system involving disinfection, in vitro multiplication, rooting, and hardening followed by ex vitro acclimatization procedures used to attain that objective, thus producing thousands of Aloe vera