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Douglas W. Maxwell and R. Daniel Lineberger

Research was undertaken to optimize seed storage and vegetative propagation of Camptotheca acuminata. Camptotheca is a member of the Nyssaceae native to southern China and is important because it contains the medicinal alkaloid camptothecin. Seeds stored in polyethylene bags in a refrigerator (4 °C) or freezer (- 20 °C) maintained good germination (79% and 83%, respectively), while seeds stored at room temperature in open containers or polyethylene bags lost germination ability quickly (45% and 51%, respectively). Softwood cuttings of Camptotheca rooted readily in intermittent mist (4 s on every 6 min.) in coarse vermiculite when treated with K-IBA (indolebutyric acid, potassium salt) quick dips ranging from 1000 to 9000 ppm, with a 7000 ppm quick dip (5 s) promoting 82% rooting with little foliar damage. Actively growing shoot tip explants were tissue cultured on media containing Murashige and Skoog, Gamborg, and Woody Plant Medium (WPM) salts in factorial combinations with BA (benzyladenine). WPM containing 1.0 mg/lBA promoted excellent shoot proliferation; microcuttings were rooted, acclimated, and grown on in the greenhouse. Camptotheca is readily adaptable to modern nursery techniques for either seed or vegetative propagation.

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Lisa W. Alexander, Anthony Witcher, and Michael A. Arnold

’ mother plant in 1992 yielded 90% to 100% rooting with 5000 and 10,000 mg·L −1 K–IBA (potassium salts of indole butyric acid; Sigma-Aldrich Chemical, St. Louis, MO) in a 50% peatmoss: 50% perlite substrate using 10 cm terminal cuttings collected on 15 Apr

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the average daily temperature was below 73 °F. AUXIN AND WOUNDING INCREASE ROOTING OF PRICKLY-PEAR CACTUS CLADODES Lazcano et al. (page 99 ) report that the use of auxin [indole-3-butyric acid potassium salt (K-IBA)] and certain

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Eugene K. Blythe and Jeff L. Sibley

shoots in early March and treated with a 5-s basal quick-dip in a solution of the potassium (K) salt of indole-3-butyric acid (K-IBA) at 3000 ppm. Dirr and Heuser (1987) reported that plants could be successfully propagated using cuttings made as late

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Scott E. Renfro, Brent M. Burkett, Bruce L. Dunn, and Jon T. Lindstrom

can be achieved with a high rate of success from softwood cuttings taken in the spring or summer, dipped in 1000 ppm K-IBA, and placed under intermittent mist in perlite or other similar medium. Rooting usually takes less than 2 weeks

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Miles Schwartz Sax, Nina Bassuk, and Mark Bridgen

produced, and root length. K-IBA vs. IBA. For two genotypes (06-1500-6 and 06-1821-3), an experiment was conducted to determine the effectiveness of K-IBA compared with IBA for the root induction. The K-IBA rooting medium was the same as a standard rooting

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Todd J. Rounsaville, Darren H. Touchell, Thomas G. Ranney, and Frank A. Blazich

solutions of K-IBA. Microcuttings were arranged in a completely randomized design with six replications and five subsamples per replication. Cell trays and rooting substrate were identical to the aforementioned experiment. Microcuttings were set under

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William A. Smith and Mark H. Brand

Propagation of ‘Summer Skies’ from softwood cuttings taken during the spring or summer can be achieved quickly and with a high rate of success. Cuttings treated with 1000 ppm K-IBA and placed under intermittent mist typically root in less than 2 weeks. Rooted

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Abigail R. Debner, Harlene Hatterman-Valenti, and Fumiomi Takeda

two different plant ages. The quick dip auxin treatments followed traditional standards and consisted of 1000-ppm (0.1%), 3000-ppm (0.3%), 5000-ppm (0.5%), and 8000-ppm (0.8%) liquid solutions of either aqueous potassium salt IBA [K-IBA (Sigma

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Xiaoming Wang, Jianjun Chen, Huijie Zeng, Zhongquan Qiao, Yongxin Li, Neng Cai, and Xiangying Wang

developed for this cultivar. For cutting propagation, the basal end of single-eye or multiple-eye cuttings should be dipped in 2000 mg·L −1 K-IBA (potassium salt indole-3-butyric acid) solution for 40 s and then stuck in a soilless substrate such as 50