relationships among plants. The amplified fragment length polymorphism (AFLP) technique ( Vos et al., 1995 ; Zabeau and Vos, 1993 ), in particular, has been used by many scientists to distinguish between species as well as cultivars of the same species ( DeHaan
Ryan N. Contreras, Thomas G. Ranney, Susana R. Milla-Lewis, and G. Craig Yencho
Rose Palumbo, Wai-Foong Hong, Guo-Liang Wang, Jinguo Hu, Richard Craig, James Locke, Charles Krause, and David Tay
genetic similarity of the accessions through molecular characterization by target region amplified polymorphism (TRAP) markers ( Hu and Vick, 2003 ). TRAP is a technique that combines the AT- and GC-rich primers of SRAP (sequence-related amplification
Jennifer A. Kimball, M. Carolina Zuleta, Matthew C. Martin, Kevin E. Kenworthy, Ambika Chandra, and Susana R. Milla-Lewis
al., 1995 ) is a multilocus molecular marker technique that is capable of amplifying numerous loci and has high levels of polymorphism. AFLPs are commonly used for assessing genetic diversity and population structure in species with little to no
Yuan Yu, Chunxian Chen, Ming Huang, Qibin Yu, Dongliang Du, Matthew R. Mattia, and Frederick G. Gmitter Jr.
[RAPD ( Bretó et al., 2001 ; Coletta et al., 1998 ; Federici et al., 1998 ; Nicolosi et al., 2000 )], restriction fragment length polymorphism ( Federici et al., 1998 ), and amplified fragment length polymorphism ( Bretó et al., 2001 ) all have been
Ambika B. Gaikwad, Tusar Kanti Behera, Anand K. Singh, Devanshi Chandel, Jawahir L. Karihaloo, and Jack E. Staub
, however, could not give complete insight into the cultigens examined. The discriminatory power of amplified fragment length polymorphism (AFLP) is often found to be greater than that of RAPD and ISSR markers ( Powell et al., 1996 ; Vos et al., 1995 ) as a
Jinggui Fang, Jianjun Chen, Richard J. Henny, and Chih-Cheng T. Chao
marker techniques, amplified fragment length polymorphism (AFLP) is a novel polymerase chain reaction (PC)R-based assay for DNA fingerprinting and polymorphism detection ( Vos et al., 1995 ). The advantages of this technique include reproducibility, high
Deborah Pagliaccia, Georgios Vidalakis, Greg W. Douhan, Ramiro Lobo, and Gary Tanizaki
-μL volume of low-salt buffer AE. The quality and quantity was evaluated in 0.8% agarose gels stained with SYBR Green I (Molecular Probes, Eugene, OR). Amplified fragment length polymorphisms. The method of Vos et al. (1995) was used to develop the
Fanjuan Meng, Mu Peng, and Fachun Guan
crops such as apple ( Kenis and Keulemans, 2005 ), citrus ( Pang et al., 2007 ), and kiwifruit ( Prado et al., 2005 ). AFLP markers have some advantages over other molecular markers such as a high level of polymorphism, high reproducibility, a wide
Dennis J. Werner
Catalase isozymes were examined in a wide range of peach [Prunus persica (L.) Batsch] cultivars representing historical U.S. cultivars, commercial cultivars from numerous North American breeding programs, and the peach plant introduction (PI) collection. All historical peach cultivars from the United States and those released from commercial breeding programs were fixed for the slow (Cat l-2) allele, with the exception of `Belle of Georgia', `Honeyglo' nectarine, and various cultivars from the Univ. of Florida breeding program, which possessed a fast-migrating (Cat 1-l) allele in homozygous or heterozygous state. Polymorphism was revealed in the 51 peach PI clones examined, with allelic frequencies of 0.69 and 0.31 for the Cat l-2 and Cat l-1 alleles, respectively. Most PIs that originated directly from China were homozygous Cat l-l/Cat l-l, while most PI clones introduced from Europe were homozygous Cat l-2/Cat l-2. Examination of the catalase genotype of cultivars previously proposed as the possible male parent of `Belle of Georgia' (`Champion', `Early Crawford', `Late Crawford', `Oldmixion Free', and `Stump-the-World') revealed that none of these cultivars could have been the male parent of `Belle of Georgia'. Segregation data from various peach crosses was consistent with the hypothesis that catalase polymorphism could be explained by the presence of two alleles at a single locus.
Vivek Sampath and Philipp Simon
Studies of genetic variation at the DNA level in the genus Daucus have been very limited. Molecular markers based on restriction fragment length polymorphism (RPLP) have been shown to be highly useful and efficient gene markers in other plant species.
We have used a total of 20 carrot types (inbreds, varieties, species) for this study. Genomic DNA probes cloned in pGEM (Promega) plasmid of Escherichia coli were hybridized to DNA of these types digested with EcoRI and HindIII restriction enzymes. Based on 50 probe-enzyme combinations we have found RFLP variation to be extensive in Daucus, even among related cultivated genetic stocks. The implications of these results in the germplasm diversity in Daucus will be discussed.
Also, a genetic linkage map of carrot will be constructed. The map will be used to determine the genomic regions conditioning traits like root and core diameter, root length, and nematode resistance.