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Brian M. Schwartz, Ryan N. Contreras, Karen R. Harris-Shultz, Douglas L. Heckart, Jason B. Peake, and Paul L. Raymer

grown in a glasshouse was determined by staining pollen deposited onto microscope slides with 2% iodine–potassium iodide (I 2 –KI) on 30 June 2011. Quantitative measurements of percent pollen stainability or pollen diameter were not estimated in this

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Dario J. Chavez and Paul M. Lyrene

southern highbush blueberry plants were placed inside a bee-proof greenhouse. Pollen stainability. Plant material used to assess pollen fertility included two V. darrowii × V. corymbosum hybrids from crosses made in 2004, three from crosses made

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Weining Wang, Yanhong He, Zhe Cao, and Zhanao Deng

shoot using an electronic digital caliper. Pollen stainability was determined using the fluorochromatic procedure by Heslop-Harrison and Heslop-Harrison (1970) with minor modifications. Fresh pollen grains in fully opened flowers were collected and

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Sarah M. Smith and Zhanao Deng

(1973) showed that C. leavenworthii and C. tinctoria were cross-compatible after hand pollination. Their F 1 hybrids showed reduced pollen stainability when grown in a greenhouse. Meiotic chromosome configuration analyses suggested that the two

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Hirotoshi Tsuda, Hisato Kunitake, Mai Yamasaki, Haruki Komatsu, and Katsunori Yoshioka

compare plant morphological characteristics and pollen stainability of these plants and their parents. Materials and Methods Plant materials. The seeds collected from wild-type shashanbo at Yame city, Fukuoka prefecture, Japan, were sterilized with sodium

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Sarah M. Smith and Zhanao Deng

their F 1 hybrids showed reduced pollen stainability. Smith (1976) indicated the existence of several structural differences, including reciprocal translocations, between the chromosomes of COLE and COTI. Thus, some levels of male and/or female

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David A. Munter, James J. Luby, and Neil O. Anderson

be “fruiting males”) during the March–April period, forcing study from forced cuttings. Fresh pollen from the hermaphroditic group was collected in situ in May 2014. Within 1 h of collection, the pollen was stained with 0.1% aniline blue in 80

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Ryan N. Contreras and Kim Shearer

. Schematic of Galtonia candicans inflorescence. Pollen staining. Flowers were collected from randomly selected M 1 plants (25 control, 26 0.2%, 19 0.4%) in the field for a pollen-staining test as an estimate of viability. Pollen was wet mounted using a

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Junji Amano, Sachiko Kuwayama, Yoko Mizuta, Masaru Nakano, Toshinari Godo, and Hajime Okuno

well as JHS Color Chart number according to Kuwayama et al. (2005a) . Pollen fertility was evaluated by staining pollen grains with 1% (w/v) acetocarmine and described as the percentage of pollen grains with deeply stained cytoplasm ( Nakano and Mii

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Ming Cai, Ke Wang, Le Luo, Hui-tang Pan, Qi-xiang Zhang, and Yu-yong Yang

was estimated in the hybrids and parents by fluorescein diacetate staining. ‘Annabelle’ and ‘Blue Diamond’ had 76.4% and 48.4% stainable pollen, respectively. Stainable pollen ranged from 0% to 76.5% among 14 hybrids ( Table 3 ). In previous studies