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Lauren C. Garner, Vanessa E.T.M. Ashworth, Michael T. Clegg, and Carol J. Lovatt

at least one pollinizer tree. Outcrossing rates of fruit persisting to harvest. Microsatellites, DNA sequences composed of tandem nucleotide repeats, are useful as genetic markers for avocado because microsatellites are polymorphic, which

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Jiang-Chong Wu, Jing Yang, Zhi-Jian Gu, and Yan-Ping Zhang

efficient molecular markers for M. oleifera is needed. Based on codominant features and high allelic polymorphism, microsatellites [simple sequence repeats (SSRs)] have become a useful marker system in genetic diversity studies ( Walter and Epperson

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Xue-qin Wang, Yuan Huang, and Chun-lin Long

considerable morphological overlap between the species making discrimination problematic, and more than 1000 horticultural hybrids in existence ( Bean, 1976 ) showed the weakness of genetic barriers toward hybridization in this genus. Microsatellites are

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Chun-yan Yang, Yuan Huang, and Chunlin Long

of this species is important. In this study, we reported the characterization of 17 polymorphic microsatellite loci to investigate genetic structure among populations of M. lasiocarpa for further conservation genetic studies. Materials and

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Jun-Bo Yang, Hong-Tao Li, De-Zhu Li, Jie Liu, Lian-Ming Gao, De-Zhu Li, Lian-Ming Gao, and Jie Liu

differentiation ( Nybom, 2004 ). This problem does not occur with microsatellite markers. Compared with other, random fingerprinting techniques such as RAPDs, ALFPs, and ISSRs, microsatellites show numerous advantages because they are locus-specific, codominant

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Marie Pairon, Anne-Laure Jacquemart, and Daniel Potter

-scale spatial genetic structure, mating system, or gene flow among populations in both its native and introduced ranges are of interest to foresters and ecologists. Over previous decades, microsatellites have emerged as markers of choice in such analyses because

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Nian Wang, Zhang Chang Qin, Jun-bo Yang, and Jing-li Zhang

. Simple sequence repeats (SSRs; microsatellites) are the favored type of molecular marker for identifying plant germplasm ( Dikshit et al., 2007 ). From a horticultural perspective, R. delavayi provides abundant genetic resources for breeding new

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Long-na Li, Songjun Zeng, Feng Zheng, Zhi-lin Chen, Kun-lin Wu, Jian-xia Zhang, and June Duan

range of the wi1d populations. Determining the genetic diversity of the remaining wild plants is a critical step in developing effective conservation strategies for P. concolor as well as many other species. Microsatellites are ideal markers for

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Zhiyong Wang, Paul Raymer, and Zhenbang Chen

and identified 30,895 contigs containing simple sequence repeat (SSR) markers. In this study, microsatellite markers for S. helferi were identified and characterized using shotgun 454 pyrosequencing and used to analyze genetic diversity of other

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Yuan Huang, Xue-qin Wang, Chun-yan Yang, and Chun-lin Long

understood. Here, 11 polymorphic microsatellite loci of P. amethystina were developed as potential tools to investigate the genetic structures of these species. Genomic DNA was extracted from leaf tissues using the cetyltrimethyl ammonium bromide (CTAB