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Cameron Northcutt, Daniel Davies, Ron Gagliardo, Kylie Bucalo, Ron O. Determann, Jennifer M. Cruse-Sanders, and Gerald S. Pullman

% after 34 d for the eight species. Sarracenia leucophylla showed 12% germination and S. purpurea ssp. purpurea and S. purpurea spp. venosa var. burkii showed 10% and 18% germination, respectively. In vitro propagation from seed, shoot tips

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Sullivan Lynch, Rachel K. Johnston, Ron O. Determann, Jennifer M. Cruse-Sanders, and Gerald S. Pullman

Dec. 2010 from three Union County, SC, sites (SC5, SC7, SC8), stored at 22 to 25 °C, and used during winter and Spring 2011. Georgia aster seed sterilization and germination in vitro and after storage Preliminary experiments with small numbers of 2009

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Lawrence W. Zettler, Sarah B. Poulter, Kris I. McDonald, and Scott L. Stewart

of symbiotic seed germination ( Dixon, 1987 ; Ramsay and Dixon, 2003 ) was used to prompt seed germination and development in vitro. Briefly, seeds were removed from cold storage, surface sterilized in a vial containing a solution of 5 mL 6.00% NaOCl

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Margarita Pérez-Jiménez, Alfonso Guevara-Gázquez, Antonio Carrillo-Navarro, and José Cos-Terrer

, the use of tissue culture techniques arose in plant breeding in an attempt to rescue certain genotypes that, in natural conditions, would not germinate. In the case of stone fruit, the seedcoat is traditionally removed because the seed isolation from

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Maria Papafotiou and Aekaterini N. Martini

. polium has low seed germination ( Nadjafi et al., 2006 ). The latter, being an endangered species in many countries of the Middle East, where it is widely used in traditional medicine, has been studied as for its possibility for in vitro propagation from

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Guochen K. Png, Katherine S. Downes, and Beng H. Tan

effectiveness of some conventional (seeds and stem cuttings) and in vitro (micropropagation) propagation techniques and to determine the optimum conditions for these techniques. Materials and Methods Seed source. Seeds of L. macrantha were purchased from

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Katrina J.M. Hodgson-Kratky, Olivier M. Stoffyn, and David J. Wolyn

is likely due to low hydraulic conductivity and wetted seed contact area in the soil ( Hadas and Russo, 1974 ). The potential for in vitro screening methods to improve germination under water stress has been demonstrated. Two cycles of phenotypic

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Phillip A. Wadl, Timothy A. Rinehart, Adam J. Dattilo, Mark Pistrang, Lisa M. Vito, Ryan Milstead, and Robert N. Trigiano

refine the standard seed germination protocol, in vitro seed germination methodology, and vegetative propagation techniques, including in vitro multiplication of cloned plantlets, to facilitate ex situ conservation and development of a new methodology for

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Yaser Hassan Dewir, Abdulhakim A. Aldubai, Rashid Sultan Al-Obeed, Salah El-Hendawy, Mayada Kadri Seliem, and Khadija Rabeh Al-Harbi

shoot tips and nodes of D. ombet seedlings onto MS medium without plant growth regulators (PGRs) for 6 months produced sufficient stock of shoots for further experiments. Fig. 1. In vitro seed germination and shoot proliferation of Dracaena ombet

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Yujie Yang, Donglin Zhang, Zhihui Li, Xiaoling Jin, and Jinying Dong

many crops to overcome seed dormancy and shorten seed germination time ( Sharma et al., 1996 ). In vitro germination of immature embryos might be helpful to shorten breeding cycles and accelerate the breeding process. In vitro cultures, plant embryos