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Dianiris Luciano-Rosario, Luis A. Cruz-Saavedra, and Dimuth Siritunga

( Ocampo Perez et al., 2006a ). Nevertheless, at a molecular level, it has been shown that commercial papaya offers a narrow genetic basis ( Matos et al., 2013 ). In the recent past, SSR markers have become an effective method for assessment of genetic

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Julie Graham, Mary Woodhead, Kay Smith, Joanne Russell, Bruce Marshall, Gavin Ramsay, and Geoff Squire

suitable for detecting natural variation ( Graham et al., 1997 , 2003 ), but are mostly dominantly inherited and are considered an imprecise method for assessing gene flow. SSR (or microsatellites), in contrast, are multiallelic, highly polymorphic, robust

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P. Escribano, M.A. Viruel, and J.I. Hormaza

). Microsatellites or SSRs have become the markers of choice for fingerprinting purposes in most plant species ( Gupta and Varshney, 2000 ), due to their high polymorphism, codominance, and reproducibility. Recently, a set of SSRs was reported for the first time in

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Liwang Liu, Guang Liu, Yiqin Gong, Wenhao Dai, Yan Wang, Fanmin Yu, and Yunying Ren

from both parents, identification of male and female parent-specific markers will allow differentiation of true hybrids from selfed parental lines and outcrossed lines. Molecular markers, such as RAPD, ISSR, SSR, SRAP, AFLP (amplified fragment length

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Maomao Ding, Ke Wang, Wenting Wang, Miaojin Chen, Dajun Wu, Changjie Xu, and Kunsong Chen

cultivars has instead been achieved by using various molecular makers. For example, using restriction fragment length polymorphism and random amplified polymorphic DNA ( Chaparro et al., 1994 ; Quarta et al., 1998 ; Rajapakse et al., 1995 ). SSR markers

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Aoxue Wang, Fanjuan Meng, Xiangyang Xu, Yong Wang, and Jingfu Li

the demand for controlling leaf mold disease on tomatoes in these provinces. We developed molecular markers to the Cf-6 gene by the means of rapid amplification of polymorphic DNA (RAPD) and simple sequence repeat (SSR). These two methods, based on

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Metka Sisko, Branka Javornik, Aleksander Siftar, and Anton Ivancic

-Corvo et al., 2000 , 2002 ; Shengua et al., 2002 ) for the identification and estimation of the genetic similarity among pear species and cultivars. Regarding SSR, Yamamoto et al. (2001) , screened apple ( Malus L.)-derived microsatellite markers in 36

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Li Lu, Kirk W. Pomper, Jeremiah D. Lowe, and Sheri B. Crabtree

( Pomper et al., 2003 )], and SSR ( Pomper et al., 2010 ). Overall, these studies determined that the genetic variation in both cultivated and wild pawpaws is similar to those of other long-lived, temperate woody perennials characterized by a widespread

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Tim Rinehart, Cecil Pounders, and Brian Scheffler

Crapemyrtles (Lagerstroemia) are deciduous shrubs or trees with prolific summer flowers. Their popularity is due in large part to low maintenance requirements in sunny climates, wide range of growth habits, disease resistance, and bark characteristics, as well as having a long flowering period (up to 120 days). Once well-established, they are extremely tolerant to heat and drought. Lagerstroemia was first introduced to the southern U.S. from southeast Asia more than 150 years ago, and is comprised of at least 80 known species. Most modern cultivars are L. indica and L. fauriei hybrids. L. speciosa is a tropical crapemyrtle with very large flowers, but lacks cold hardiness. It is a vigorous plant, but only when grown in Hardiness zones 9 or 10. We recently established microsatellite markers for Lagerstroemia and evaluated their utility for verifying interspecific hybrids. Here we verify F1 hybrids between L. indica `Tonto', `Red River', and L. speciosa. We also genotyped two commercially available L. speciosa hybrids. Currently, we are using crapemyrtle SSRs for cultivar identification and germplasm conservation. Future research includes marker-assisted breeding to produce powdery mildew and flea beetle resistant cultivars, as well as improved growth habit and fall foliage color.

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Beibei Li, Xiucai Fan, Ying Zhang, Chonghuai Liu, and Jianfu Jiang

)], intersimple sequence repeat ( Jing and Wang, 2013 ; Zhang et al., 2011 )], sequence-related amplified polymorphism (SRAP) ( Liu et al., 2013 ), and SSR ( Jing et al., 2013 ; Liu et al., 2012 )], have been used recently to examine the genetic diversity and