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Sara Melito, Angela Fadda, Emma Rapposelli, and Maurizio Mulas

demonstrated that intersimple sequence repeat and AFLP could discriminate the genotypes according to their geographic origin ( Agrimonti et al., 2007 ; Bruna et al., 2007 ; Melito et al., 2013b ). Among the genetic markers, the AFLPs have some advantages: a

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Jonathan D. Mahoney, Thao M. Hau, Bryan A. Connolly, and Mark H. Brand

al., 2013 ; Robertson et al., 2010 ; Smolik et al., 2011 ). AFLP analysis often has been preferred over other molecular methods for its efficiency ( Leonard et al., 2013 ; Lubell et al., 2008 ; Obae and Brand, 2013 ). The AFLP technique has proven

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Luwbia Aranda, Timothy G. Porch, Mark J. Bassett, Laura Lara, and Perry B. Cregan

. Circumlineatus phenotype with precipitation line indicated by the arrow in representative lines from the t z cl G b v virgarcus BC 3 5-593 × t z sel Cl G b v sellatus BC 3 5-593 population of common bean. AFLP and bulk segregant analysis. DNA was

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M.A. Dalbó, G.N. Ye, N.F. Weeden, W.F. Wilcox, and B.I. Reisch

., for assistance with the AFLP analysis. The cost of publishing this paper was defrayed in part by the payment of page charges. Under postal regulations, this paper therefore must be hereby marked advertisement solely to indicate this fact.

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Eder J. Oliveira, Maria Lucia C. Vieira, Antonio Augusto F. Garcia, Carla F. Munhoz, Gabriel R.A. Margarido, Luciano Consoli, Frederico P. Matta, Michel C. Moraes, Maria I. Zucchi, and Maria Helena P. Fungaro

) and AFLP markers ( Carneiro et al., 2002 ; Lopes et al., 2006 ) for the parents of a full-sib family derived from a cross between the Brazilian accessions IAPAR-123 and IAPAR-06. The homology of these linkage groups (LGs) was established using AFLP

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Maria José Aranzana, Joaquim Carbó, and Pere Arús

A sample of 210 cultivars of Prunus persica (L.) Batsch, with a wide range of fruit and plant characteristics, was studied for variability using nine polymorphic amplified fragment length polymorphism (AFLP) primer combinations. Forty-seven AFLPs allowed identification of 196 (93%) different genotypes, 187 of which could be distinguished with three primer combinations. Eleven cultivars with the same AFLP phenotype corresponded to known somatic mutations (sports), but from the four sports of the `Springcrest' group, two (`Maycrest' and `Queencrest') differed at three AFLPs from the others (`Starcrest' and `Early Maycrest'). Cluster analysis allowed differentiation of most cultivars with nonmelting fruit flesh, generally used for canning, from the melting-flesh peach and nectarine cultivars used for fresh consumption.

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Chalita Sriladda, Heidi A. Kratsch, Steven R. Larson, and Roger K. Kjelgren

extracted with the DNeasy 96 Plant Kit (Qiagen, Valencia, CA). AFLPs were assayed as described by Vos et al. (1995) with described modifications. The DNA samples were preamplified with EcoRI +1/MseI +1 using A/C selective nucleotides. Selective

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Amnon Levi and Claude E. Thomas

sequence repeat (ISSR), amplified fragment length polymorphism (AFLP), and sequence-related amplified polymorphism (SRAP) markers ( Levi et al., 2006 ). In this study, markers from different linkage groups of the watermelon genetic linkage map ( Levi et

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Boyang R. Cao and Chih-Cheng T. Chao

Polymorphisms of 21 date cultivars (Phoenix dactylifera L.) in California were determined by amplified fragment length polymorphism (AFLP) analysis with near infrared fluorescence labeled primers. Four primer sets were used to detect polymorphisms. Based on the UPGMA-cluster analysis of 328 polymorphic bands, the majority of the cultivars was separated into two major groups. Cultivars Abada, Amir Hajj, Ashrasi, Bentamoda, Boyer No. 11, Deglet Beida, Horra, Javis No. 1, Khadrawy, and Thoory belonged to group I. Cultivars Badrayah, Dayri, Halawy, Haziz, Khir, Medjool, Sayer, and Zahidi belonged to group II. Cultivars Barhee and Deglet Noor were further separated from groups I and II. `Hayany' was distinct from all other date cultivars tested. These results demonstrated that AFLP markers could efficiently identify individual date cultivars. The information will be useful for future date germplasm collection and facilitated selection of diverse parents for cross hybridization in a breeding program.

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F.J. Keiper and R. McConchie

Umbrella fern [Sticherus flabellatus (R. Br.) St John] is a successful Australian native foliage product. Currently, all umbrella fern sold on the market is bush-harvested. To meet the growing demand for this product on local and international markets, a commercially viable method for its production must be developed, with effective management of the germplasm resource in terms of conservation and exploitation. To manage this resource, breeders require a detailed knowledge of the amount and distribution of genetic variability within the species. Traditionally, plant breeders focus on a combination of agronomic and morphological traits (phenotype) to measure genetic diversity. In umbrella fern there are a limited number of morphological traits, and these are influenced by environmental factors and therefore do not reflect true genetic diversity. To overcome these problems, molecular techniques such as PCR-based DNA markers are used to complement traditional strategies for genotype assessment. DNA markers have the advantages of being independent of environmental effects, as well as being fast, cost-effective, reproducible, and largely accessible to the nonmolecular geneticist. Amplified fragment length polymorphisms (AFLPs) fulfil many of the desirable features of molecular markers, as well as requiring little knowledge of the genome to be investigated. AFLPs have been used widely in the analysis of breeding systems, ecogeographical variation, and genetic variation within and between natural populations. To date there are no published accounts of DNA molecular marker research on umbrella fern. A DNA extraction protocol has been developed for this species, and AFLP markers have been used to analyse genetic diversity within and between natural populations sampled in the Sydney Basin. A large number of polymorphic loci were revealed using 11 primer combinations. The genetic variation detected was partitioned between rather than within populations, suggesting that the mating system in Sticherus is primarily inbreeding. Data will be presented illustrating AFLPs as useful molecular markers for assessing genetic diversity within and between populations of umbrella fern and providing insight on the breeding system used by the species.