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Xiaojuan Zong, Brandon J. Denler, Gharbia H. Danial, Yongjian Chang, and Guo-qing Song

incorporate target genes of interest for its further improvement. To achieve this, an efficient in vitro shoot regeneration system is needed. Successful in vitro shoot regeneration from seed-derived or leaf explants has been reported for several Prunus

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Fatemeh Haddadi, Maheran Abd Aziz, Hossein Kamaladini, and Seyed Ali Ravanfar

shoot regeneration in cultivated strawberry was demonstrated using several types of in vitro cultured explants. However, the highest regeneration success was obtained from leaf explants for most of the cultivars analyzed ( Passey et al., 2003

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Elisabeth M. Meyer, Darren H. Touchell, and Thomas G. Ranney

features. Development of in vitro regeneration systems provides an ideal foundation for further improvements by ploidy manipulations, mutation treatments, and transgenic applications. Previous in vitro regeneration studies of Hypericum have focused on

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Cary J. Hebert, Darren H. Touchell, Thomas G. Ranney, and Anthony V. LeBude

R . ‘Fragrant Affinity’ may restore fertility ( Contreras et al., 2007 ). In vitro regeneration protocols provide an excellent mechanism for the manipulation of ploidy level, mutation treatment, and transgenic applications. In vitro shoot

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Ilse-Yazmín Arciniega-Carreón, Carmen Oliver-Salvador, María-Guadalupe Ramírez-Sotelo, and Carlos Edmundo Salas

been developed for regeneration of cucurbitaceous species, like cucumber ( Cucumis sativus ) ( Kim et al., 2010 ; Kumar et al., 2003a ), winter squash ( Cucurbita maxima Duch.) ( Lee et al., 2003 ), ash gourd ( Benincasa hispida ) ( Thomas and

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Carol Gonsalves, Baodi Xue, and Dennis Gonsalves

1 Permanent address: Dept. of Plant Protection, Nanjing Agricultural Univ., Nanjing 210014, People's Republic of China. We thank P. Chee for answering questions on squash regeneration, F. Klas for providing the

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G.G. Ning and M.Z. Bao

vitro culture systems have been developed for various species of the Prunus genus. The majority of these have been achieved by inducing regeneration from either immature tissues ( Hashmi et al., 1997 ; Hokanson and Pooler, 2000 ; Mante et al., 1989

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Sadiye Hayta, Mark A. Smedley, Jinhong Li, Wendy A. Harwood, and Philip M. Gilmartin

( Schween and Schwenkel, 2003 ) regenerated plantlets from pedicle explants. Although reasonably efficient, the obligate requirement for floral material to initiate culture greatly limits this system. The limited amount of floral material produced per plant

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Yu-Tsung Lin, Chia-Wei Lin, Chien-Hung Chung, Mei-Hsiu Su, Hsiu-Yin Ho, Shi-Dong Yeh, Fuh-Jyh Jan, and Hsin-Mei Ku

regeneration procedures for C. metuliferus vary greatly with respect to the genotype of plants, the explant sources, and growth regulators used ( Adelberg, 1998 ; Beharav and Cohen, 1994 ; McCarthy et al., 2001 ; Punja et al., 1990 ; Raharjo and Punja

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Ling Yu, Hongwei Chen, Peipei Hong, Hongli Wang, and Kefeng Liu

on temperature, which adds difficulties on its reproduction ( Lai et al., 2001 ). Thus, it is of great importance to investigate proper rapid propagation technique of S. splendens . Recently, an indirect regeneration method that uses the callus of S