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Erik C. Fargo and Jeffrey A. Adkins

Poster Session 17—Ornamental Plant Breeding 2 28 July 2006, 1:15–2:00 p.m.

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Maria Cantor

Poster Session 17—Ornamental Plant Breeding 2 28 July 2006, 1:15–2:00 p.m.

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Margaret R. Pooler

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Loren C. Stephens

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Sandra M. Reed and Margaret R. Pooler

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Sandra M. Reed, Gary R. Bachman, and W. Edgar Davis

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Loren C. Stephens

Immature inflorescences of a Sorghastrum nutans (L.) Nash selection were cultured on CCm medium with 5 mg·L−1 2,4-dichlorophenoxyacetic acid and 1 mg·L−1 N6-benzyladenine (BA) for 5 weeks. Callused inflorescence cultures were placed on CCm medium with 1 mg·L−1 BA (CCmB1) and 0 or 250 mg·L−1 ethidium bromide (EtBr) for 24 h. Cultures were transferred to CCmB1 without EtBr for shoot regeneration and then to CCm without plant growth regulators for rooting. Rooted shoots were transferred to soil under greenhouse conditions and then to the field. Fifteen putative M1 mutants with atypical phenotypes were detected among 71 EtBr-treated regenerants. Two self-incompatible putative M1 mutants were progeny-tested by using a wild-type Indiangrass seedling as the pollen parent. M1 selection ISU06-35 was a dwarf mutant whose M2 testcross progeny segregated 1:1 tall:dwarf seedlings. M1 selection ISU06-56 was a red-flowered mutant whose M2 testcross progeny segregated 1:1 green-flowered:red-flowered seedlings. These results are consistent with both M1 mutants being dominant nuclear mutations.

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Xinwang Wang, Deborah Dean, Phillip Wadl, Denita Hadziabdic, Brian Scheffler, Timothy Rinehart, Raul Cabrera, and Robert Trigiano

Lagerstroemia L. (crape myrtle) is an economically important woody plant genus with several deciduous flowering ornamental species. A wide range of flower colors, long flowering periods, growth habits ranging from miniature to tree sizes, and exfoliating bark characteristics provide horticulturists and nursery growers with a great deal of interest in the breeding and genetics of this genus. We report microsatellite marker development from a GT-enriched genomic library of the interspecific hybrid ‘Natchez’ (L. indica L. × L. fauriei Koehne). Twelve of 43 novel microsatellite loci were characterized on a collection of 33 Lagerstroemia cultivars and accessions. Four to eight alleles per locus (mean = 5.6 alleles) were detected. Allelic richness ranged from 3.9 to 7.2 with a mean of 5.3. The level of polymorphism detected (average gene diversity of 0.68) indicates moderately high genetic diversity within the selections of crape myrtle cultivars and accessions. The examined markers also exhibited high cross-species transferability to L. fauriei, L. limii Merr., and L. subcostata Koehne.