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cultivation in a controlled environment containing atmospheric 13 CO 2 and to characterize labeled compounds by high-performance liquid chromatography–tandem mass spectrometry (HPLC-MS/MS). Due to our particular interest in anthocyanins, we have chosen red

extracted and quantified using high-performance liquid chromatography following the “short run” protocol ( Collins et al., 1995 ). Capsaicin and dihydrocapsaicin were determined and pooled as total capsaicinoids. Capsaicinoid yield was calculated based on

Lakes, NJ) before high-performance liquid chromatography (HPLC) analysis. High-performance liquid chromatography pigment analysis. An Agilent 1200 series HPLC unit with a photodiode array detector (Agilent Technologies, Palo Alto, CA) was used

malvidin ( m/z 331) glycosides. Fig. 1. Blueberry anthocyanin profiles. ( A ) High-performance liquid chromatography chromatograph of blueberry anthocyanins. All peaks were detected at ultraviolet absorbance of 520 nm. Labels 1 to 18 on peaks correspond to

serum using high performance liquid chromatography and its correlation with spectrophotometric Caska-Slov-Farm. 434 166 168 Criqui, M.H. Ringel, B.L. 1994 Does diet or alcohol explain the French paradox Lancet 344 1719 1723 Dragovic-Uzelac, V. Levaj, B

filter paper (Toyo Roshi Kaisha, Tokyo, Japan), placed on a plastic tube, and stored at 2 °C in the absence of light until analysis. High-performance liquid chromatography-mass spectrometry conditions. The reverse-phase high-performance liquid

(10 μL) were analyzed by high-performance liquid chromatography (HPLC) using a Shimadzu Prominence system with Shimadzu refractive index detector and a 300 × 7.8-mm Rezex ROA-organic acid column (Phenomenex, Torrance, CA) with 0.004% HPLC-grade formic

estimate carotenoid content and concentration would be beneficial. High-performance liquid chromatography (HPLC) is used to chemically analyze tissues for carotenoid types and concentrations ( Gross, 1991 ). It is labor-intensive and expensive but a

measured in the middle of each petal (three replicates per flower; 10 flowers per repetition) to ensure equal measurement conditions. Extraction and high-performance liquid chromatography determination of phenolic compounds. Flower petals (combined samples

separated, dried over anhydrous magnesium sulfate, and evaporated using a rotary evaporator (≈5 mm Hg, 20 to 30 °C). The residue was dissolved in 0.5 mL methanol/chloroform (1:2 by volume) and the BSLs separated using high-performance liquid chromatography