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D.G. Ranamukhaarachchi, R.J. Henny, C.L. Guy, and Q.B. Li

Randomly amplified polymorphic DNA (RAPD) markers were utilized to determine the genetic relationships of nine morphologically similar pot plant cultivars of Anthurium sp. by developing DNA fingerprints (DFP). Of 25 arbitrary primers screened, nine generated DFPs that were used in computing the genetic distance (d) and similarity coefficient (C) values. All cultivars tested exhibited a high degree of genetic similarity. `Lady Ann' and `Lady Beth' possessed the closest relationship with d and C values of 0.06 and 0.98, respectively. The next closest genetic relationship was between `Red Hot' and `Southern Blush' (d = 0.33, C = 0.89). These two cultivars exhibited a more distant relationship to the other seven cultivars as indicated by higher `d' values. However, this study showed that the nine Anthurium cultivars examined were genetically closely related. These cultivars share specific DNA bands with three possible parental species (A. andraeanum Linden ex Andre, A. antioquens L., and A. amnicola Dressler) included in this study, which may indicate similarities in their pedigree. This study shows that RAPDs can be a useful tool to distinguish Anthurium pot plant cultivars as well as identify their genetic relationships.

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Fei-Yun Zhuang, Jin-Feng Chen, Jack E. Staub, and Chun-Tao Qian

The current Cucumis taxonomic classification places C. hystrix Chakr. in subgen. Cucumis based on its morphological similarities to cucumber (C. sativus L., 2n = 14). However, the chromosome number of C. hystrix was identified as 2n = 24, the same number as in subgen. Melo. Cucumis hystrix is therefore considered the first wild Cucumis species of Asiatic origin possessing 12 basic chromosomes. Thus, any research regarding its biosystematics would challenge the basic chromosome number and geographic location theories that govern the current taxonomic system. The production of the amphidiploid species (Cucumis ×hytivus Chen and Kirkbride, 2n = 38) obtained from the cross between C. hystrix and C. sativus and subsequent chromosome doubling would provide an effective means of investigating the relationship between Cucumis species with two different basic chromosome numbers. Thus, RAPD markers were used to study the taxonomic placement of C. hystrix and its interspecific hybrid with cucumber. Of the 220 arbitrary primers screened, 31 were used for analysis where 402 (96.3%) fragments were polymorphic among the germplasm examined. A UPGMA-based cluster analysis partitioned 31 accessions into two main groups [C. sativus (CS) and C. melo (CM)]. Under the similarity coefficient threshold of 0.23, these two groups can be further divided into five clusters with C. hystrix, C. ×hytivus, and C. sativus as separate clusters in the CS group. A modified taxonomic system is proposed based on these results and findings of a previous chloroplast DNA analysis with the genus Cucumis containing subgen. Cucumis with three species and subgen. Melo with six series.

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Rose Palumbo, Wai-Foong Hong, Guo-Liang Wang, Jinguo Hu, Richard Craig, James Locke, Charles Krause, and David Tay

single PCR reaction, and that previously reported genetic information has the potential to be used as the targeted primer. Each of the two arbitrary primers has a different fluorescent label, so the PCR-amplified DNA fragments can be detected by two

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Zhanao Deng, Fahrettin Goktepe, Brent K. Harbaugh, and Jinguo Hu

development. Four of the primers were called “fixed” primers, and the other four were called “arbitraryprimers. Fixed primers were designed against the annotated expressed sequence tag (EST) of sunflower (name starts with “QH”) or lettuce (name starts with

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Kai-Ge Zhao, Ming-Qin Zhou, Long-Qing Chen, Donglin Zhang, and Gituru Wahiti Robert

, indicating two cases of homonymy existed. Random amplified polymorphic DNA marker analysis. Of 180 arbitrary primers screened in this study, 19 were selected because of their ability to produce reproducible and well-defined bands. A total of 165

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Xiaobo Zhang, Derong Su, Luyi Ma, and Yan Zhao

amplified polymorphic DNA (RAPD) marker analysis ( Williams et al., 1990 ), based on a polymerase chain reaction (PCR) with arbitrary primers, is not influenced by the environment and is used effectively for analyzing genetic diversity in various grasses

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Xiaohe Song and Zhanao Deng

described by Hu and Vick (2003) and Song et al. (2012) . In this marker system, one fixed primer was paired with two arbitrary primers in each PCR, and amplified DNA fragments were detected by the infrared (IR) dye attached to the arbitrary primer ( Hu

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Ivan Simko and Jinguo Hu

genotyping carried out on the cultivar Paragon (J. Hu, unpublished). Marker data were obtained from 18 primer pair combinations (all, but Lac08 of Hu et al., 2005 ), each consisting of a fixed primer and an arbitrary primer. The fixed primers were designed

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Margaret T. Mmbaga, and Roger J. Sauvé,

Fingerprinting genomes using PCR with arbitrary primers Nucl. Acids Res. 18 6531 6535 Windham, M.T. Trigiano, R.N. 1998 Are ‘Barton’ and ‘Cloud 9’ the same cultivar of Cornus florida? J. Environ. Hort. 16 163 168

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Vidyasagar R. Sathuvalli, Shawn A. Mehlenbacher, and David C. Smith

Ooijen, J.W. Voorrips, R.E. 2006 JoinMap 4.0, Software for the calculation of genetic linkage maps Kyazama Wageningen, The Netherlands Welsh, J. McClelland, M. 1990 Fingerprinting genomes using PCR with arbitrary primers Nucleic Acids Res. 18 7213 7218