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Rongcai Yuan, Walter J. Kender, and Jacqueline K. Burns

The effects of removal of young fruit and application of auxin transport inhibitors on endogenous indole-3-acetic acid (IAA) and abscisic acid (ABA) concentrations were examined in relation to the response of mature `Valencia' orange [Citrus sinensis (L.) Osb.] fruit to abscission materials. ABA concentrations were increased in the fruit abscission zone and pulp but not in the pedicel, peel, or seed of mature fruit by removal of young fruit during the period of reduced response of mature fruit to abscission materials in early May. However, removal of young fruit slightly decreased IAA concentrations in leaves and the abscission zone and pedicel of mature fruit but had no effect on the IAA concentrations in the peel, pulp, or seed of mature fruit. Young fruit had higher IAA concentrations in the abscission zone and pedicel than mature fruit. Application of 2,3,5-triiodobenzoic acid (TIBA), an IAA transport inhibitor, reduced IAA concentrations in the abscission zone of mature fruit but did not influence the IAA concentrations in the pedicel and peel when applied directly to an absorbent collar tied around the pedicel 2 cm above the fruit abscission zone during the less responsive period in early May. ABA concentrations were increased drastically in the fruit abscission zone and pedicel but not in peel by TIBA application. Applications of ABA, or IAA transport inhibitors such as naringenin, quercetin, or TIBA comparably increased the response of mature fruit to the abscission material 5-chloro-3-methyl-4-nitro-1 H-pyrazole (CMN-pyrazole) in early May. These data suggest that young fruit reduce the response of mature `Valencia' oranges to abscission materials through increasing IAA concentrations and decreasing ABA concentrations in the abscission zone of mature `Valencia' orangees.

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Rongcai Yuan, Ulrich Hartmond, Angela Grant, and Walter J. Kender

Influence of young fruit, shoot, and root growth on response of mature `Valencia' oranges [Citrus sinensis (L.) Osbeck] to the abscission chemical CMN-pyrazole was examined in 1999 and 2000. CMN-pyrazole dramatically increased ethylene production in mature fruit and reduced the fruit detachment force (FDF), except during a period of reduced response to CMN-pyrazole in early May when spring vegetative growth, young fruit of the following year's crop, and mature fruit were all on the trees. Removal of spring flushes, which included spring vegetative shoots and leafy and leafless inflorescences, prevented any young fruit and shoot growth, but did not inhibit root growth. However, trunk girdling in combination with removal of spring flushes not only prevented growth of young fruit and shoots but also inhibited root growth. During the responsive period, there were no differences in either ethylene production or FDF of CMN-pyrazole-treated mature oranges between 1) the nonmanipulated trees and those manipulated by either 2) removal of spring flushes alone, or 3) in combination with trunk girdling. However, during the less responsive period, ethylene production in CMN-pyrazole-treated mature oranges was significantly lower while the FDF was higher in nonmanipulated trees than in trees treated by either removal of spring flushes alone, or in combination with trunk girdling. There was no difference in either fruit ethylene production or FDF between trees manipulated by (2) removal of spring flushes alone, and (3) removal of spring flushes in combination with trunk girdling regardless of CMN-pyrazole application. Shoot growth terminated at least 2 weeks before the onset of the less responsive period. Removal of young fruit increased response of mature fruit to CMN-pyrazole during the less responsive period. This suggests that hormones from rapidly growing young fruit may be responsible for the occurrence of the less responsive period. Chemical name used: 5-chloro-3-methyl-4-nitro-1H-pyrazole (CMN-pyrazole).

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J.K. Burns, C. Arias, I. Kostenyuk, and M. Obraztnova

The process of abscission results in shedding of plant parts such as leaves, fruit, flowers, and in citrus, shoot tips and entire shoots. Growers must successfully manage abscission in their operations to avoid unnecessary defoliation or loss of yield due to floral abscission or preharvest fruit drop. Conversely, abscission enhancement may be desired during harvest. Yet despite its importance to horticulture, little is known about mechanisms that control abscission. We know that abscission can be induced by ethylene and altered to some extent by auxin. Over the years, many physiological and anatomical events of abscission have been described. For example, cellulase, polygalacturonase and pectin methylesterase genes are induced during abscission, and they are thought to have a role in alteration and depolymerization of middle lamella polysaccharides located in the abscission zone area. Other genes, such as those associated with the process of pathogen resistance, are also induced during abscission. We are interested in using tools of molecular biology to examine abscission-related gene expression prior to organ separation in Florida field-grown Valencia orange (Citrus sinensis L. Osbeck) and greenhouse-grown calamondin (Citrus madurensis Loureiro) citrus trees. Subtractive cDNA library screening and differential display were used to examine gene expression in fruit, leaf and floral abscission zones 6, 24 and 48 h after induction of abscission with 5-chloro-3-methyl-4-nitro-1H-pyrazole or Ethrel® (Rhone-Poulenc, [2-chloroethyl] phosphoric acid). Some isolated cDNAs encoded polypeptides with no significant matches in the database or share significant similarities with unknown proteins isolated from Arabidopsis. Other cDNAs encoded polypeptides with similarity to cell wall modifying proteins such as polygalacturonases and expansin, PR proteins such as chitinase, proteins associated with secondary and xenobiotic metabolism such as amine oxidase, benzoquinone reductase, caffeic acid methyltransferase, phenylalanine ammonia lyase and squalene synthase, and proteins associated with signal transduction such as several serine/threonine kinases. Temporal and spatial expression of these genes and others will be presented. Use of this information to target potential points of abscission control will be discussed.

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Matthew W. Fidelibus, Karen E. Koch, and Frederick S. Davies

enhancement with 5-chloro-3-methyl-4-nitro-1H-pyrazole) ( Alferez et al., 2006 ). Gibberellic acid maintained sucrose gradients in the peel. An intriguing aspect of the GA 3 response observed here was that the consistent reduction in sucrose depletion

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Biwek Gairhe, Peter Dittmar, Davie Kadyampakeni, Ozgur Batuman, Fernando Alferez, and Ramdas Kanissery

Alferez, F. Pozo, L. Burns, J.K. 2006 Physiological changes associated with senescence and abscission in mature citrus fruit induced by 5-chloro-3-methyl-4-nitro-1H-pyrazole and ethephon application Physiol. Plant. 127 66 73

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Muhammad Farooq, Masoud Salyani, and Jodie D. Whitney

Field experiments were conducted to investigate the effect of sprayer type, airflow rate, and nozzle output on deposition of active ingredient and mechanical harvesting of `Valencia' orange (Citrus sinensis). Fruit detachment force (FDF) and percentage of fruit removal (PFR) by trunk shaker were used as mechanical harvesting parameters. A PowerBlast sprayer discharging radially and a Titan sprayer discharging over the entire canopy were used. The spray mixture contained an abscission chemical (CMN-pyrazole), a surfactant (Kinetic) and a fluorescent tracer (Pyranine-10G). Deposition was determined at three different heights outside and inside of the canopy. With the PowerBlast, higher airflow and lower nozzle output reduced deposition of the active ingredient. The mean FDF of sprayed treatments was less than that of the non-sprayed control but the difference among the four spray treatments was not significant. The lower airflow rate with lower nozzle output had higher PFR than that of the control. With the Titan sprayer, the mean deposition at lower airflow was similar to or higher than the higher airflow. At higher airflow, the lower nozzle output gave higher mean deposition. The Titan sprayer treatments resulted in less FDF than the control. At both airflow rates, the FDF was less at lower nozzle output than at higher nozzle output. The PFR of these treatments were not different from that of control.

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Kelly T. Morgan, Smita Barkataky, Davie Kadyampakeni, Robert Ebel, and Fritz Roka

-methyl-4-nitro-1H-pyrazole (CMNP)] and harvested with a trunk shaker at low speed and hand-harvesting did not reduce yield the next year. However, the use of CMNP and trunk shakers at high speed was found to reduce following-year yields in the same study

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Eliezer E. Goldschmidt

-loosening chemicals. Recent research by Burns and coworkers has focused on CMNP (5-chloro-3-methyl-4-nitro-1H-pyrazole) as a promising fruit-loosening agent ( Alferez et al., 2007 ). High concentrations of 2,4-D are applied in the packing house, often together with GA

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Karthik-Joseph John-Karuppiah and Jacqueline K. Burns

J. Expt. Bot. 53 2039 2055 Alferez, F. Singh, S. Umbach, A.L. Hockema, B. Burns, J.K. 2005 Citrus abscission and arabidopsis plant decline in response to 5-chloro-3-methyl-4-nitro-1 H