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Santosh Shiwakoti, Henry Y. Sintim, Shital Poudyal, Jennifer Bufalo, Charles L. Cantrell, Tess Astatkie, Ekaterina Jeliazkova, Lyn Ciampa, and Valtcho D. Zheljazkov

a dryer at 65 °C until constant weight was achieved. Distillation. The 72 dried Japanese cornmint biomass samples were steam-distilled for 60 min in 2-L steam distillation units to extract the essential oil as described previously ( Gawde et al

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Shahrokh Khanizadeh and Andre Bédanger

Leaves of three strawberry cultivars (Bounty', `Honeoye', and `Kent') were collected at random from plants growing in an experimental trial at the Agriculture Canada, Research Station farm at Lavaltrie, Quebec. Steam-distillation was carried out on 300g of leaves in 3L of distilled water in a 5L flask. The essential oils were analyscd with a Varian 6000 gas chromatogmph. Thirty-seven compounds were detected of which sixteen were identified. The major components were linalool and nonanal. Many of the other constituents were aliphatic in nature. Differences in oil composition among the three cultivars were observed. Essential oil composition might therefore be used as a selection criteria for insect or disease resistance. Their effect upon mites will be assayed in future studies by testing them as sex, food, or oviposition lures.

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W. Letchamo, C. Mengle, and A. Gosselin

The content of essential oil, thymol, and carvacrol in a thymol-type of clonally selected thyme plants during different developmental stages were investigated under greenhouse and field conditions. Plants in the greenhouse were grown from July to November, under natural light and natural light supplemented by a PPF of 200 μmol·m–2·s–1, provided by HPS lamps, while plants in the field were studied from June to November. Shoot yield and the accumulation of the active principles from greenhouse-grown plants were determined by harvesting the plants at 40-, 60-, and 120-day intervals, while field-grown plants were harvested in August, September, October, and November. Essential oil content, qualitative and quantitative changes in the oil were determined by subjecting the samples to steam distillation and subsequent gas chromatographic analysis. There were important changes in shoot yield, essential oil, thymol, and carvacrol content in the course of plant development. After 120 days of growth under greenhouse conditions, the essential oil content increased by >150%, while thymol content increased by ≈200% compared with the 40-day-old plants. We found some differences in oil content, thymol, and carvacrol accumulation between field- and greenhouse-grown plants. The pattern of crop yield and the accumulation of the major active substances under field and greenhouse conditions are presented and discussed.

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Valtcho D. Zheljazkov, Tess Astatkie, Thomas Horgan, Vicki Schlegel, and Xavier Simonnet

cultivated in large areas of eastern Europe (Bulgaria, Hungary, and Romania), and the essential oil is extracted through steam distillation of the biomass ( Nedkov and Attanassova, 2004 ). The essential oil of A. annua has applications in perfumery and

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Valtcho D. Zheljazkov and Tess Astatkie

Japanese cornmint and develop a regression model that describes their relationship. Materials and Methods Steam distillation and distillation times. Japanese cornmint cultivar Arvensis 2 was used in this study. A Japanese cornmint plantation with ‘Arvensis

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Valtcho D. Zheljazkov, Tess Astatkie, and Ekaterina Jeliazkova

essential oil composition of Rocky Mountain juniper within one male and one female trees. Materials and Methods Plant material and essential oil steam distillation. All samples used in this study were generated from two adjacent large Rocky Mountain junipers

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Valtcho D. Zheljazkov, Santosh Shiwakoti, Tess Astatkie, Ivan Salamon, Daniela Grul'ová, Silvia Mudrencekova, and Vicki Schlegel

the EO is synthesized and stored inside the seed in special canals called vitae, steam distillation of the whole seeds may take several hours and may require substantial energy input ( Atanassova and Nedkov, 2004 ). None of the literature reports had

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Valtcho D. Zheljazkov, Tess Astatkie, and Vicki Schlegel

this study was to evaluate the effect of DT (5, 10, 20, 40, 80, 160, 240, and 360 min) on yield, composition, and the antioxidant activity of ponderosa pine essential oil. Material and Methods Steam distillation and distillation times. Fresh pine

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Valtcho D. Zheljazkov, Tess Astatkie, and Ekaterina Jeliazkova

derived by steam distillation as described previously ( Zheljazkov et al., 2012a , 2012b ). The EO solutions were prepared using 5 mL of either sagebrush or juniper oil plus 5 g of Tween20 dissolved in 1 L water. The MJ (Sigma-Aldrich, St. Louis MO

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Valtcho D. Zheljazkov, Tess Astatkie, and Ekaterina Jeliazkova

July (2 DAH). Samples that were drying under direct sun were not protected from the rain to simulate the drying process out in the field as is done by the growers in the mint production industry. Distillation. All 42 samples were extracted using steam