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Pablo Bolaños-Villegas, Shih-Wen Chin, and Fure-Chyi Chen

standard for comparison of pollen division patterns. Table 1. Genome constitution and cytological characteristics of nine Doritaenopsis hybrids. Pollen staining with fluorescein diacetate (FDA). Pollen viability analyzed by staining

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Hazel Y. Wetzstein, Nadav Ravid, Erik Wilkins, and Adriana Pinheiro Martinelli

continue down the length of the style ( Fig. 6E ). Aniline blue staining of tissues observed under ultraviolet light shows the path of pollen tubes throughout much of the stigma ( Fig. 6D ) but which become directed to grow only within the central stylar

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Joshua H. Kardos, Carol D. Robacker, Michael A. Dirr, and Timothy A. Rinehart

. angustipetala . Pollen viability. Pollen viability was assessed using a fluorescein diacetate (FDA) staining procedure developed by Heslop-Harrison and Heslop-Harrison (1970) . Flowers were collected on the day of anthesis from five randomly chosen hybrids per

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Michele A. Stanton, Joseph C. Scheerens, Richard C. Funt, and John R. Clark

, fully stained) or nonviable (uninfused, lightly stained, or abnormally shaped). From among the 140 flowers, a subset of 48 randomly selected flowers (six per cultivar per chamber) was simultaneously examined for pollen germinability. Pollen from 10

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Hongli Wei, Chao Gao, Jie Qiu, Li Long, Biao Wang, Lu Yang, and Yang Hu

floral organs are prone to frostbite, which seriously hinders pollen germination, pollination, and fertilization. Plant flowering biology is the basis of plant reproduction. Conducting studies on flowering biological characteristics is of great

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Carlee Steppe, Sandra B. Wilson, Zhanao Deng, Keri Druffel, and Gary W. Knox

lantana in Australia also differed dramatically in pollen viability and ploidy level/chromosome number. The weedy form had ≈65% pollen stainability and was a tetraploid with 2 n = 4 x = 48 chromosomes, whereas the garden form had extremely low pollen

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Ryan N. Contreras, Thomas G. Ranney, and Shyamalrau P. Tallury

assessed using staining and germination tests ( Sharma and Sharma, 1980 ). Pollen was collected from diploid and allotetraploid plants, dried at 25 °C for 24 h, and frozen at –25 °C. Staining was performed by adding 1% acetocarmine (w/v) solution and

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Haiyan Xu, Folian Li, Yuezhi Pan, and Xun Gong

at 60 °C for 4 h after being rinsed with distilled water five times. After maceration, the materials were rinsed again and stained with 0.01% aniline blue for 24 h. Slides were mounted with glycerol and kept in darkness. Pollen grain germination and

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Todd J. Rounsaville, Darren H. Touchell, and Thomas G. Ranney

cultivated (mounded) rows with 1.2 m between rows and irrigated as needed throughout the summer using drip irrigation. Evaluating male fertility. Male fertility was assessed by estimating the viability of fresh pollen with acetocarmine staining. Pollen was

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Md. Mizanur Rahim Khan, Mst. Hasnunnahar, and S. Isshiki

width were measured in units of an ocular micrometer that was calibrated with a calibration slide. Pollen stainability in acetocarmine, pollen diameter of stained pollen, and in vitro germination ability of pollen were investigated for assessing the